Article
Chemistry, Applied
Ruifeng Ying, Ting Zhou, Hui Xie, Meigui Huang
Summary: The variability in nutrient content in wheat grains differs by region due to environmental factors. Arabinoxylan (AX), the main component of wheat grain cell wall, is essential for determining dough and flour quality. In this study, AX was extracted from 10 wheat genotypes originating from different regions, with the largest molecular weight difference observed between KaifengAX and Yangzhou-AX. The properties of AX and/or (1,3)(1,4)beta-glucan (BG) flour products were analyzed, including molecular, functional, and nutritional properties, and it was found that higher AX molecular weight in bread resulted in a lower starch hydrolysis rate. Additionally, bread samples containing AX and BG exhibited the lowest hydrolysis rate, indicating a synergistic effect of these polysaccharides on starch hydrolysis rate.
FOOD HYDROCOLLOIDS
(2023)
Article
Biotechnology & Applied Microbiology
Hamed I. Hamouda, Yi-Xuan Fan, Mohnad Abdalla, Hang Su, Ming Lu, Fu-Li Li
Summary: Lam16A is a GH16 beta-1,3-1,4-lichenase with potential in promoting the availability of beta-1,3-glucan oligosaccharides for health benefits. The six carbohydrate-binding modules (CBMs) in its catalytic module, particularly Lam16A-1CBM, play a crucial role in substrate specificity, thermal stability, and binding affinity. The full-length Lam16A with six CBMs exhibits excellent thermostability and high activity against certain polysaccharides, such as barley beta-glucan and laminarin.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Environmental Sciences
Binita Dev, Arindam Bakshi, Balasubramanian Paramasivan
Summary: The transition towards a bio-based economy has led to an increasing demand for fresh water, making biofuel a high water footprint product. The depletion of fresh water resources has worsened the situation and requires the exploration of non-potable water for biorefinery purposes. In this study, seawater is used as an alternative reaction medium for pretreatment and saccharification of rice straw. The optimized pretreatment conditions were determined using Response Surface Methodology based on Box-Behnken Design, resulting in high cellulose release and reducing sugar yield. Physico-chemical studies confirmed the efficacy of the pretreatment by showing delignification and changes in surface morphology of the biomass. This finding suggests the potential of utilizing abundantly available seawater as a reaction medium for sustainable biofuel production.
Article
Food Science & Technology
Julia Steiner, Michael Kupetz, Thomas Becker
Summary: This study focuses on extracting beta-glucan and arabinoxylan from brewer's spent grain using hydrothermal treatment. The results show that high yields of fiber can be obtained with the right process settings, and a larger proportion of desirable fiber fractions can be achieved at lower temperatures.
Article
Biochemistry & Molecular Biology
C. E. Klostermann, P. H. van der Zaal, H. A. Schols, P. L. Buwalda, J. H. Bitter
Summary: In this study, the reaction dynamics of the GTFB enzyme were investigated using isoamylase debranched starches as simplified linear substrates. The research demonstrated that the presence of alpha-(1 -> 4) linked malto-oligomers DP < 6 led to higher GTFB transferase activity, while their absence resulted in higher GTFB hydrolytic activity. This information will be useful for the targeted synthesis of IMMPs in the future.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Agricultural Engineering
Yuanhang Ai, Shengqiu Feng, Youmei Wang, Jun Lu, Mengdan Sun, Huizhen Hu, Zhen Hu, Ran Zhang, Peng Liu, Hao Peng, Yanting Wang, Limin Cao, Tao Xia, Liangcai Peng
Summary: This study successfully improved lignocellulosic substrates and increased bioethanol yields in transgenic rice through the selection of specific genes and green-style pretreatments. The results show a novel strategy for high bioethanol production by integrating genetically-improved lignocellulosic substrates with optimal one-pot-process technology in bioenergy crops.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Agricultural Engineering
Premeshworii Devi Maibam, Arun Goyal
Summary: This study successfully formulated an enzyme cocktail with optimal enzyme proportions, leading to improved degradation efficiency and total reducing sugar yield during enzymatic saccharification of biomass. This is of great significance for lignocellulosic biorefineries for bioethanol production.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Agricultural Engineering
Gaurav Sharma, Baljit Kaur, Yashika Raheja, Dhruv Agrawal, Neha Basotra, Marcos Di Falco, Adrian Tsang, Bhupinder Singh Chadha
Summary: This study developed a lignocellulolytic strain capable of efficiently hydrolyzing mild alkali deacetylated rice straw. The study also evaluated the conversion of lignin-rich black liquor obtained during rice straw pretreatment into biogas.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Morteza Maleki, Shohreh Ariaeenejad, Ghasem Hosseini Salekdeh
Summary: This study identified two cellulase enzymes with ionic liquid tolerance, which exhibited higher activity and improved stability in the presence of ionic liquids. These enzymes could enhance the saccharification rate of biomass in a one-pot wash-free method, making them attractive candidates for industrial applications.
BIORESOURCE TECHNOLOGY
(2022)
Article
Chemistry, Organic
Yuxing Cai, Jiean Chen, Yong Huang
Summary: This report describes a successful organocatalytic 1,4-alkylacylation of 1,3-enynes using a ketyl radical generated from single-electron oxidation of the Breslow intermediate as a coupling partner of allenyl radicals. The transformation exhibited remarkable tolerance to both aromatic and radical precursors.
Article
Agricultural Engineering
Antony Catherine Flora Louis, Sivakumar Venkatachalam, Sumit Gupta
Summary: Success in developing a novel natural based biodegradable starch film with nanocellulose and nanohemicellulose reinforcements using a two-step approach of microwave-assisted delignification and ultrasonic extraction. The resulting films exhibited enhanced mechanical properties and reduced water vapor permeability.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Agricultural Engineering
Premeshworii Devi Maibam, Arun Goyal
Summary: In this study, a deep eutectic solvent comprising choline chloride and acetic acid was used for the pretreatment of rice straw. The optimized conditions for pretreatment were determined using a central composite design approach. The pretreated rice straw showed higher cellulose content and crystallinity index, and exhibited a porous surface. Saccharification of the pretreated rice straw using commercial cellulase resulted in high saccharification efficiency.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Song Tang, Yan-ling Yu, Rukuan Liu, Shenghua Wei, Qin Zhang, Jie Zhao, Song Li, Qian Dong, Yan-bin Li, Yuanli Wang
Summary: Ethylene glycol and ferric chloride pretreatment assisted by low-pressure carbon dioxide achieved targeted deconstruction of lignocelluloses, with 98% cellulose recovery, 92% lignin removal and 90% hemicellulose removal. The pretreatment led to the formation of stable mono phenol components, destruction of lignin-carbohydrate complexes structure, roughening of rice straw surface, and increased enzyme adsorption rate by 8.1 times. Furthermore, glucose yield of pretreated straw was significantly increased by 5.6 times and reached 91% after hydrolysis for 48 hours. The addition of Tween 80 in concentrated solid hydrolysis reduced half of the hydrolysis time and increased glucose yield by 45%.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Bhanu Pratap Prajapati, Naveen Kango
Summary: This study evaluated the recyclability of alkali black liquor (ABL) in the pretreatment of rice straw, and found that it can be reused for at least up to five consecutive cycles without adverse effects on delignification and saccharification. The results suggest that ABL has the potential to significantly reduce water and land pollution, highlighting its importance for environmental sustainability.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Agricultural Engineering
Helena Poy, Estela Lladosa, Adrian Arcis, Carmen Gabaldon, Sonia Loras
Summary: This study investigates a new green and cost-effective biomass pretreatment method using microwave irradiation and a ternary deep eutectic solvent (DES) for the efficient conversion of rice straw. The results show that under mild pretreatment conditions, the DES can achieve high delignification and nearly complete conversion of polysaccharides in the rice straw, indicating its potential for improved valorization of biomass polysaccharides.
INDUSTRIAL CROPS AND PRODUCTS
(2023)