Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 73, Issue 9, Pages 2131-2135Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.90299
Keywords
yeast; stress tolerance; proline; trehalose; PRO1
Categories
Funding
- Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN)
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Proline is considered to be a stress protectant in the yeast Saccharomyces cerevisiae, as is trehalose. In this study, we constructed yeast strains that accumulate proline but not trehalose and that increase proline levels in response to stress. Our results suggest that proline does not complement the function of trehalose, and that moderate expression of the PRO1 gene encoding gamma-glutamyl kinase is important to stress tolerance of yeast cells.
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