Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 72, Issue 4, Pages 1130-1133Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.70813
Keywords
plasmid; electrporation; Sphingobium; Novosphingobium
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We isolated and characterized two small cryptic indigenous plasmids, pYAN-1 (4,896bp) and pYAN-2 (4,687bp), from Sphingobium yanoikuyae, and developed a versatile system that permitted genetic manipulation of the genus Sphingomonas. Nucleotide sequencing of both plasmids revealed that they contained mobA, mobs, and repA genes, which are predicted to encode proteins associated with mobilization and replication, in common. Transformation with each plasmid harboring the antibiotic resistance gene by electroporation was fully successful, using Novosphingobium capsulatum as a host.
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