Review
Genetics & Heredity
Elena Palomo-Rios, Isabel Narvaez, Fernando Pliego-Alfaro, Jose A. Mercado
Summary: Olive is crucial oil crop in Mediterranean region, and genetic transformation technology has great potential to enhance olive cultivation, while optimization of protocols is key to success.
Review
Plant Sciences
Aparajita Choudhury, Manchikatla Rajam
Summary: Genetic transformation in legume plants is an important area of agricultural research. Despite some progress in improving crop yields, challenges such as recalcitrance and genotype-dependence still exist. Future research may focus on overcoming these obstacles and further increasing yields.
PLANT CELL REPORTS
(2021)
Review
Plant Sciences
Madhu Tiwari, Arun Kumar Mishra, Debasis Chakrabarty
Summary: Plants respond to Agrobacterium infection by mounting a three-layered immune response, which determines their susceptibility or resistance to the pathogen. Understanding the molecular dialogues between plants and Agrobacterium may provide important insights for the treatment of crown gall disease.
Article
Plant Sciences
Antonio Calabuig-Serna, Ricardo Mir, Rosa Porcel, Jose M. Segui-Simarro
Summary: Brassica napus is a species of high agronomic interest commonly used for studying various processes, including microspore embryogenesis. DH4079 and DH12075 lines, with high and low embryogenic response respectively, are ideal for investigating the mechanisms controlling embryogenesis induction. This study examined the genetic transformation ability of DH4079 plants and compared it with the already studied DH12075 plants. Results showed that DH12075 plants responded to genetic transformation, while DH4079 plants remained recalcitrant, failing to produce any regenerant. The study also tested transient transformation with Agrobacterium rhizogenes, where only DH12075 showed a response. These findings indicate that DH4079 is recalcitrant to Agrobacterium-mediated transformation.
Review
Horticulture
Iftekhar Alam, Md Salimullah
Summary: Eggplant is a significant vegetable in Asia and the Mediterranean, but faces limitations in productivity due to pests and environmental stresses. Genetic engineering offers new traits to enhance eggplant, but further research is needed to maximize benefits.
Review
Green & Sustainable Science & Technology
Bimal Kumar Ghimire, Chang Yeon Yu, Won-Ryeol Kim, Hee-Sung Moon, Joohyun Lee, Seung Hyun Kim, Ill Min Chung
Summary: Genetic transformation is an important tool for improving plants by incorporating new genes with desirable traits. It can increase crop yields, disease resistance, and tolerance to environmental stress, as well as improve biomass and medicinal values of plants. The introduction of genetically modified food brings potential risks, so proper assessment of their impact and biosafety is necessary. There are controversies regarding the use of GMOs, especially related to human health, nutrition, and environmental concerns. More research and detailed studies are needed for the advancement of genetic transformation.
Article
Plant Sciences
Kuang-Teng Wang, Ming-Chang Hong, Yu-Sheng Wu, Tsung-Meng Wu
Summary: Duckweed is one of the smallest flowering plants in the world and is used for livestock as biofactors and feedstuff additives due to its high reproduction rate and biomass. This study successfully established a micropropagation technique and Agrobacterium-mediated transformation in duckweed.
Article
Plant Sciences
Huimin Ren, Yan Xu, Xiaohong Zhao, Yan Zhang, Jamshaid Hussain, Fuqiang Cui, Guoning Qi, Shenkui Liu
Summary: Casuarina equisetifolia is widely used for agroforestry plantations for various purposes. This study established an efficient regeneration system using stem segment explants, optimizing callus induction and regeneration processes. Genetic transformation protocol using Agrobacterium tumefaciens was also optimized, leading to improved transformation efficiency to obtain transgenic plants in about 4 months. The findings will pave the way for further genetic manipulation and functional genomics of C. equisetifolia.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Michelle M. M. Dominguez, Carmen S. S. Padilla, Kranthi K. K. Mandadi
Summary: Developing an efficient transformation system is crucial for genetically engineering recalcitrant crops, especially trees. In this study, we present a stable plant transformation method for citrus genetic engineering using Agrobacterium tumefaciens. The methodology has been optimized for fourteen citrus varieties and includes infection, co-cultivation, selection, and culture media conditions. The transformation efficiency can reach 11%, and transgenic plants can be obtained in as early as six months, depending on the variety. The incorporation of helper virulence genes and antioxidants improves the transformation efficiency of specific recalcitrant cultivars.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Hu Xu, Yong Guo, Lijuan Qiu, Yidong Ran
Summary: Soybean, as an important crop for food, feed, and biofuel, has been genetically modified for trait improvement for more than 4 decades. However, compared to other major crops, soybean still faces challenges in genetic transformation. In recent years, progress has been made through modifications in tissue culture conditions and understanding of transformation mechanisms, achieving high efficiency and genotype flexibility.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Chemistry, Multidisciplinary
Jihong Tan, Lili Lin, Haiyan Luo, Shengjun Zhou, Yuqiang Zhu, Xin Wang, Li Miao, Huasen Wang, Peng Zhang
Summary: This article systematically reviews the key factors influencing the regeneration and genetic transformation of cucumber plants and focuses on recent advances in the study of important agronomic traits using genetic transformation technology.
APPLIED SCIENCES-BASEL
(2022)
Review
Biochemistry & Molecular Biology
Saleem Ur Rahman, Muhammad Omar Khan, Rahim Ullah, Fayaz Ahmad, Ghulam Raza
Summary: Plant transformation through Agrobacterium-mediated transformation is a technique that mimics the natural agrobacterium system to introduce gene(s) into crops. It has shown successful genetic transformation in various crop species for different traits. Although it has advantages like stable transgene integration and cost-effectiveness, limitations such as crop recalcitrance, low transformation efficiency, transgene integration, and off-target effects exist. Therefore, it is crucial to explore the limitations and possible solutions to enhance the efficiency of Agrobacterium-mediated transformation.
MOLECULAR BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Xiaonan Li, Haiyan Li, Yuzhu Zhao, Peixuan Zong, Zongxiang Zhan, Zhongyun Piao
Summary: This study developed a regeneration system for Chinese cabbage and established a simple and efficient genetic transformation method. The method can obtain transformants in a relatively short period of time, paving the way for further gene editing and functional studies.
HORTICULTURAL PLANT JOURNAL
(2021)
Article
Plant Sciences
Shengnan Liu, Yunlu Shi, Fang Liu, Yan Guo, Minhui Lu
Summary: This study presents an optimized transformation system that improves the infection and genetic transformation frequency of immature maize embryos mediated by Agrobacterium tumefaciens. The use of LaCl3 pretreatment enhances the infection frequency, increases the proportion of positive callus, yields more positive regenerated plantlets, and significantly increases the transformation frequency from 8.40% to 17.60%.
PLANT CELL REPORTS
(2022)
Article
Horticulture
Junxia Zhang, Jieping Wu, Xinyi Hao, Yaping Xie, Kai Lv, Weirong Xu
Summary: This study established a stable and reproducible transformation protocol for grapevine, using immature zygotic embryos. The optimized conditions led to a considerable reduction in the time required for transformation and the method was found to be easily accessible and straightforward to implement.
SCIENTIA HORTICULTURAE
(2023)