Journal
BIORESOURCE TECHNOLOGY
Volume 154, Issue -, Pages 282-289Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.12.059
Keywords
Cellulase production; T. reesei RUT-C30; A. saccharolyticus; beta-Glucosidase; Wet explosion
Funding
- National Advanced Biofuels Consortium (NABC)
- Department of Energy, USA [ZFT04064401]
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Cellulase production by two filamentous fungi Trichoderma reesei RUT-C30 and novel fungal strain, Aspergillus saccharolyticus on pretreated corn stover was investigated. Cellulase production was followed by the hydrolysis of two feedstocks, wet-exploded corn stover (WECS) and wet-exploded loblolly pine (WELP) by on-site produced enzyme cocktails containing cellulase from T. reesei RUT-C30 and beta-glucosidase from A. saccharolyticus. The sugar yields using the on-site enzyme cocktails were compared with commercial enzymes preparations, Celluclast 1.5L and Novozym 188 at two substrate concentrations, 5% and 10% (w/w) and enzyme loading at 5 and 15 FPU/g glucan for WECS and WELP. The highest sugar yields were obtained at 5% (w/w) substrate concentration and 15 FPU/g glucan for both feedstocks. Glucose yields of 81% and 88% were obtained from on-site and commercial enzymes, respectively using WECS as feed stock. The sugar yields were 55% and 58% for WELP samples hydrolyzed with on-site and commercial enzymes, respectively. (C) 2014 Published by Elsevier Ltd.
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