Article
Chemistry, Applied
Daniela Couto, Tania Melo, Tiago A. Conde, Ana S. P. Moreira, Paula Ferreira, Margarida Costa, Joana Silva, Rosario Domingues, Pedro Domingues
Summary: This study evaluated the efficiency of lipid extraction from Chlorella vulgaris and compared the effects of food-grade ethanol, ultrasound-assisted ethanol, and dichloromethane/methanol. The results showed that ultrasound-assisted ethanol and dichloromethane/methanol had the highest extraction yields, and the fatty acid profiles were identical among all extracts. However, the polar lipidome of the ethanol extracts was significantly different from the other two extracts. These findings are important for obtaining high yields of omega-3 and omega-6-rich extracts from C. vulgaris for food applications.
Article
Engineering, Chemical
Wenlu Song, Rui Huang, Hao Guo, Chunguang Yin, Chuanling Wang, Jun Cheng, Weijuan Yang
Summary: Steam pretreatment was used to disrupt microalgal cells for lipid extraction, resulting in distorted cell walls due to hydrolysis of organic macromolecules. Increasing pretreatment temperature and time enhanced the hydrolysis and eventually destroyed microalgal cell walls. Lipid extracted from wet microalgal significantly increased after pretreatment.
CHINESE JOURNAL OF CHEMICAL ENGINEERING
(2021)
Article
Chemistry, Applied
Pantea Moradi, Majid Saidi
Summary: This study utilized Chlorella Vulgaris microalgal as a renewable oil feedstock for biodiesel production through electrochemical and thermal processes. The results showed that the electrochemical process had higher biodiesel production yield compared to the thermal process, and was able to produce biodiesel in the presence of water. By adding H2SO4 in the electrochemical process, the biodiesel production yield could be increased to 95% under the optimal conditions.
FUEL PROCESSING TECHNOLOGY
(2022)
Article
Energy & Fuels
Yuxiang Mao, Jun Cheng, Hao Guo, Yang Shao, Lei Qian, Weijuan Yang
Summary: A bifunctional catalyst with synergetic Lewis and Bronsted acid sites was synthesized by modifying zeolitic imidazolate framework (ZIF-90) with sulfamic acid (SA), which enhanced the conversion efficiency of microalgal lipids into biodiesel.
Article
Energy & Fuels
Mysoon M. Al-Ansari, Latifah Al-Humaid, Nora Dahmash Al-Dahmash, Majdoleen Aldawsari
Summary: Biodiesel is an effective replacement for fossil fuels and research is ongoing to improve its quality. Adding nanoparticles to biodiesel is one method for improving its quality and various tests are conducted to evaluate the efficiency and emissions of the fuel blends produced. The energy values and exergy of these fuel blends are more important than the performance, emissions, and combustion levels tested.
Article
Chemistry, Medicinal
Savvas Giannis Mastropetros, Eleni Koutra, Mohammed Amouri, Majda Aziza, Sameh Samir Ali, Michael Kornaros
Summary: This study investigated the performance of two Chlorella strains under different nitrogen levels for ammonia stripping. The local isolate showed better growth than the industrial strain, resulting in higher biomass production. High initial ammonia concentration led to increased protein content, while low nitrogen levels favored fatty acid accumulation.
Article
Agricultural Engineering
Chithirai Arutselvan, Ganesan Narchonai, Arivalagan Pugazhendhi, Felix LewisOscar, Nooruddin Thajuddin
Summary: The study screened four microalgal strains and a consortium for biodiesel application, with Coelastrella sp. showing higher optical density and Chlorella vulgaris and Scenedesmus dimorphus consortium yielding higher dry cell weight. In outdoor pond raceway pond, different strains showed varying biomass yield and lipid content, indicating potential for biodiesel production.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Joran Verspreet, Christina M. Kuchendorf, Baerbel Ackermann, Leen Bastiaens
Summary: This study aimed to determine the impact of nutrient limitation and harvest methods on the preservation of Chlorella vulgaris biomass. Results showed that nutrient limitation led to lower pH values, higher levels of lactic acid and acetic acid, and slightly higher lipid hydrolysis. Well-fed algae concentrates had a higher pH and different fermentation product patterns. The impact of harvest methods was smaller, with continuous centrifugation resulting in higher levels of lactic acid and acetic acid.
BIOENGINEERING-BASEL
(2023)
Article
Environmental Sciences
Oh Kyung Choi, Jae Woo Lee
Summary: This study proposes an energy-efficient method using switchable polarity solvents for lipid recovery from microalgae. The results show that dipropylamine (DPA) is a potential solvent capable of achieving high lipid extraction yield from microalgae and efficient solvent recovery.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Rajendran Varunraj, Uthayakumar Priyadharshini, Kannusamy Vijay, Srinivasan Balamurugan
Summary: This study demonstrated the improvement of Chlorella vulgaris strain through adaptive laboratory evolution (ALE) under hypersaline conditions, resulting in increased lipid production without compromising cellular physiological parameters. The evolved strains showed higher lipid accumulation and antioxidant potential, while experiencing decreased carbohydrate and protein content.
ENVIRONMENTAL RESEARCH
(2023)
Review
Agricultural Engineering
You-Kwan Oh, Sangui Kim, Dea Prianka Ayu Ilhamsyah, Sun-Gu Lee, Jung Rae Kim
Summary: This review discusses the current achievements in cell disruption and lipid extraction methods for Chlorella species in the past five years, covering various chemical, physical, and biological approaches and comparing their advantages, limitations, and applicability. Despite future challenges in developing environmentally friendly and efficient extraction technologies, there are promising prospects for practical applications in sustainable Chlorella biorefineries.
BIORESOURCE TECHNOLOGY
(2022)
Review
Thermodynamics
Soumaya Grira, Hadil Abu Khalifeh, Mohammad Alkhedher, Mohamad Ramadan
Summary: Microalgal biofuel has the potential to replace nonrenewable fuel sources and address energy, economic, and environmental challenges. However, the low economic viability of its production process calls for interdisciplinary research to commercialize this green fuel alternative. This paper evaluates the current status of microalgal biofuel production processes, compares various cultivation modes, harvesting methods, lipid extraction methods, and conversion methods, and highlights the non-destructive lipid extraction method (milking) as superior to the conventional process. It also recommends integrating the biorefinery concept, implementing genetic engineering, and utilizing artificial intelligence and process automation for efficient technology development.
Article
Biotechnology & Applied Microbiology
Daniela Couto, Tania Melo, Tiago A. Conde, Margarida Costa, Joana Silva, M. Rosario M. Domingues, Pedro Domingues
Summary: This study characterized the polar lipidome of Chlorella vulgaris grown under autotrophic and heterotrophic conditions, revealing differences in lipid species and abundance distribution. Heterotrophic cultures had a higher abundance of low unsaturated species, while autotrophic cultures were rich in highly unsaturated glycolipids and lyso species. The fatty acids profile showed a high content of omega-3 polyunsaturated fatty acids in autotrophic cultures and omega-6 fatty acids in heterotrophic cultures. The two lipid extracts exhibited high antioxidant activity and inhibitory capacity for cyclooxygenase-2, providing evidence of polar lipid plasticity in microalgae for biotechnology applications.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Agricultural Engineering
Mikyoung Jung, Young-Eun Kim, Nakyeong Lee, Hyoji Yu, Jiye Lee, Soo Youn Lee, Young-Chul Lee, You-Kwan Oh
Summary: Magnesium aminoclay nanoparticles (MgANs) have both positive and negative effects on photosynthetic microalgae. This study investigated the use of MgANs in producing algal lipids under high CO2 concentrations. Among three oleaginous Chlorella strains, only KR-1 showed significant improvement in lipid content and extraction efficiency in the presence of MgANs. This improvement was due to increased biosynthesis of triacylglycerols and a thinner cell wall.
BIORESOURCE TECHNOLOGY
(2023)
Article
Energy & Fuels
Jessica Martin, George P. P. Philippidis
Summary: The study found that using agricultural residues as cheap and sustainable sources of fermentable carbohydrates can increase biomass and product yields of microalgae. By cultivating Chlorella vulgaris in the hydrolysate of Brassica carinata residue, the algae showed higher lipid and lutein productivity compared to expensive commercial media. Time course studies revealed the effect of culture medium on lipid and lutein biosynthesis, and the fatty acid profile looked promising for biodiesel production. Inexpensive hydrolysate may be a viable alternative for algae cultivation.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Chemistry, Multidisciplinary
Jeong-Cheol Seo, Hyunjoung Kim, Yeol-Lim Lee, Sungchan Nam, Hyun-Seog Roh, Kyubock Lee, Seung Bin Park
Summary: This study successfully prepared mesoporous supported nickel catalysts with high activity and stability in DRM reaction through a one-pot synthesis method. By using spray pyrolysis-assisted evaporation-induced self-assembly, the catalysts with highly dispersed nickel active sites were obtained in one step. Furthermore, the performance of nickel catalysts was further optimized by employing phase segregation and exsolution strategies.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Osama M. Darwesh, Ibrahim A. Matter, Hesham S. Almoallim, Sulaiman A. Alharbi, You-Kwan Oh
APPLIED SCIENCES-BASEL
(2020)
Article
Nanoscience & Nanotechnology
Bon-Jun Ku, Byoung-Min Lee, Dong Hyun Kim, Anush Mnoyan, Sung-Kwon Hong, Kang Seok Go, Eun Hee Kwon, Shin-Hyun Kim, Jae-Hak Choi, Kyubock Lee
Summary: The new concept of SEOA combining solar-driven evaporation and adsorption is proposed for efficient oil-spill remediation. The photothermal fabrics significantly enhance oil removal, removing 95% of the crude oil in experiments. This approach shows potential for minimizing environmental disaster through fast and efficient oil-spill remediation.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Hana-Nur Fitriana, Soo-Youn Lee, Sun-A Choi, Ji-Ye Lee, Bo-Lam Kim, Jin-Suk Lee, You-Kwan Oh
Summary: This study investigated the technical feasibility of inducing astaxanthin biosynthesis in H. pluvialis using electric stimulation in a two-chamber bioelectrochemical system. An optimal voltage of 4 V (anodic current: 30 mA) effectively stimulated astaxanthin biosynthesis, resulting in a 36.9% increase in astaxanthin content in treated cells compared to untreated cells. Proper regulation of bioelectrochemical parameters can improve astaxanthin production in H. pluvialis culture using electric treatment.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Multidisciplinary
Hana Nur Fitriana, Jiye Lee, Sangmin Lee, Myounghoon Moon, Yu Rim Lee, You-Kwan Oh, Myeonghwa Park, Jin-Suk Lee, Jinju Song, Soo Youn Lee
Summary: This study utilized an electroactive bacterium Rhodobacter sphaeroides for microbial electrosynthesis, enhancing the efficiency of CO2 reduction reaction and achieving higher yields by modifying the cathode material.
APPLIED SCIENCES-BASEL
(2021)
Article
Electrochemistry
Van-Toan Nguyen, Gyoung-Ja Lee, Quang-Tung Ngo, Oleksii Omelianovych, Ngoc-Anh Nguyen, Van-Huy Trinh, Ho-Suk Choi, Anush Mnoyan, Kyubock Lee, Liudmila L. Larina, Guangliang Chen
Summary: A rational top-down method was proposed to construct a hybrid structure of nickel encased by a carbon shell (Ni@C) as highly active and durable non-noble metal electrocatalysts for the hydrogen evolution reaction (HER) in an acidic environment. The optimized Ni@C (15%) core-shell catalyst demonstrated the highest catalytic activity with an overpotential of 85 mV and a low Tafel slope of 44 mV dec(-1). Ni@C (15%) also showed superior stability during continuous HER chronoamperometry for over 24 hours and remained active after being submerged in 2 M H2SO4 solution for 168 hours.
ELECTROCHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Ahmed S. Yasin, Ahmed Yousef Mohamed, Donghyun Kim, Sungmin Yoon, Howon Ra, Kyubock Lee
Summary: The study designed AC/Fe2O3 nanoparticles as electrodes in CDI, showing promising performance in the process.
Article
Chemistry, Multidisciplinary
Nakyeong Lee, Aditya Lakshmi Narasimhan, Gyuseop Moon, Young-Eun Kim, Myeonghwa Park, Bolam Kim, Rendi Mahadi, Sungwook Chung, You-Kwan Oh
Summary: This study explores the technical feasibility of room-temperature extraction of astaxanthin using a combination of ultrathin alpha-quartz nanoplates (NPLs) and ionic liquids (ILs). The results show that NPLs can effectively disrupt the cell wall of cysts, thereby improving the extraction efficiency of astaxanthin.
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Multidisciplinary
Rendi Mahadi, Laxmi Priya Sathiya Vahisan, Dea Prianka Ayu Ilhamsyah, Sangui Kim, Bolam Kim, Nakyeong Lee, You-Kwan Oh
Summary: The effects of a phytohormone rac-GR24 on the growth and biosynthesis of astaxanthin and fatty acids in Haematococcus pluvialis were investigated. The results showed that the addition of rac-GR24 at an appropriate concentration improved biomass production and the production of astaxanthin and fatty acids.
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Physical
Seung Bo Kim, Ahmed Al-Shahat Eissa, Min-Jae Kim, Emad S. Goda, Jae-Rang Youn, Kyubock Lee
Summary: A facile and green synthetic strategy was developed to construct an efficient Ni@CeO2 catalyst for the dry reforming of methane. The catalyst showed superb syngas production efficiency and stability, with resistance to carbon deposits and sintering. It exhibited high catalytic activity and durability during the DRM reaction.
Article
Chemistry, Physical
Mamoona Waris, Howon Ra, Sungmin Yoon, Min-Jae Kim, Kyubock Lee
Summary: This study investigates the effect of citric acid concentration on the preparation of highly dispersed Ni catalysts on mesoporous silica for the dry reforming of methane. The addition of citric acid during catalyst synthesis results in highly dispersed Ni particles and improves catalytic performance. However, too high of a citric acid concentration leads to the formation of larger Ni particles.
Article
Agricultural Engineering
Mikyoung Jung, Young-Eun Kim, Nakyeong Lee, Hyoji Yu, Jiye Lee, Soo Youn Lee, Young-Chul Lee, You-Kwan Oh
Summary: Magnesium aminoclay nanoparticles (MgANs) have both positive and negative effects on photosynthetic microalgae. This study investigated the use of MgANs in producing algal lipids under high CO2 concentrations. Among three oleaginous Chlorella strains, only KR-1 showed significant improvement in lipid content and extraction efficiency in the presence of MgANs. This improvement was due to increased biosynthesis of triacylglycerols and a thinner cell wall.
BIORESOURCE TECHNOLOGY
(2023)
Article
Engineering, Environmental
Siranuysh Badalyan, Anush Mnoyan, Alexey Sobolev, Iana S. Soboleva, Igor A. Presniakov, Jae-Rang Youn, Taeyoul Han, Dongyeon Kim, Kyubock Lee
Summary: Iron saccharide complexes based on sucrose and fructose ligands are reported as efficient electrocatalysts for electrochemical nitric oxide reduction reaction (e-NORR) at ambient conditions, showing high efficiency and stability.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Applied
Dong Hyun Kim, Jeong-Cheol Seo, Yong Jun Kim, Jeongmin Kim, Sungmin Yoon, Howon Ra, Min-Jae Kim, Kyubock Lee
Summary: This study developed a series of Ni-Co alloy catalysts using the exsolution method and explored the optimal Ni/Co ratio for dry reforming methane (DRM) reaction. The catalysts exhibited improved activation balance in methane and carbon dioxide, excellent coking resistance, and reaction pathway efficiency by incorporating Co as a secondary metal and precisely controlling its proportion. The Ni4Co1 catalyst showed excellent coke resistance with the lowest coking rate of only 0.34% compared to the monometallic Ni catalyst. The findings of this study provide insights into the fabrication of high-performance DRM catalysts utilizing the exsolution-driven alloying technique.
Article
Chemistry, Physical
Hyunjoung Kim, Ahmed Al-Shahat Eissa, Seung Bo Kim, Hongjin Lee, Woohyun Kim, Dong Joo Seo, Kyubock Lee, Wang Lai Yoon
Summary: The newly synthesized mesoporous Ni/MgAl2O4 spinel catalyst exhibits high efficiency and durability in steam methane reforming reaction, with superb resistance to carbon deposition. The catalyst's structure and catalytic activity can be tuned by controlling annealing conditions.
CATALYSIS SCIENCE & TECHNOLOGY
(2021)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)