Journal
BIORESOURCE TECHNOLOGY
Volume 165, Issue -, Pages 205-213Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.03.132
Keywords
Poplar; AFEX; Acetylxylanesterase (Axe); GH43 hemicellulase; Bioreactor
Funding
- Indian Institute of Technology Guwahati through Ministry of Human Resource and Development (MHRD), Government of India, New Delhi, India
- Department of Biotechnology (DBT), Ministry of Science and Technology, New Delhi, India [BT/23/NE/TBP/2010]
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Bioethanol was produced from poplar leafy biomass rich in hemicelluloses content involving recombinant Clostridium thermocellum hemicellulases and pentose sugar utilizing Candida shehatae. FT-IR analysis revealed effective AFEX pretreatment of poplar leaves. Repetitive batch strategy yielded similar to 1.5-fold rise in cell biomass and specific activity of both, acetylxylanesterase (Axe) and GH43 hemicellulase. TLC and HPAEC exhibited xylose and arabinose release from hydrolyzed biomass. SSF trial with 1% (w v (1)) pretreated poplar and mixed enzymes showed similar to 1.5-fold higher ethanol titre as compared with SHF. The shake flask SSF with 5% (w v (1)) pretreated poplar furnished 4.56 and 5.43 g L (1) ethanol with Axe and mixed enzymes, respectively. Whereas, bioreactor scale-up exhibited similar to 1.25-fold increase in ethanol titres (5.68, 6.75 g L (1)) as compared with shake flask with an yield of 0.295 (g g (1)) and 0.351 (g g (1)), respectively with Axe and mixed enzymes. (C) 2014 Elsevier Ltd. All rights reserved.
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