Article
Chemistry, Medicinal
Xiaohong Chen, Biswarup Sen, Sai Zhang, Mohan Bai, Yaodong He, Guangyi Wang
Summary: Thraustochytrid strain PKU#SW8 demonstrated optimal growth and DHA accumulation under specific culture conditions, with monosaccharides and glycerol proving to be more effective for enhancing cell mass and DHA concentration. Organic nitrogen sources also played a beneficial role in improving cell mass and DHA concentration.
Article
Environmental Sciences
Shuangfei Li, Boyu Wang, Liangxu Liu, Yingjie Song, Chuhan Lv, Xingyu Zhu, Yanqing Luo, Christopher H. K. Cheng, Huapu Chen, Xuewei Yang, Tao Li
Summary: The study demonstrates that adding Aurantiochytrium sp. to diets can significantly improve the growth performance and immune response of Trachinotus ovatus. With increased microalgae content, the growth rate and feed utilization efficiency of fish were enhanced, along with increased fatty acid content in blood and tissues, resulting in improved immunity and disease resistance in fish.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Environmental Sciences
Shitong Liang, Xuewei Yang, Xingyu Zhu, Muhammad Ibrar, Liangxu Liu, Siting Li, Xia Li, Tian Tian, Shuangfei Li
Summary: Disrupting the fatty acid beta-oxidation pathway through metabolic engineering in Aurantiochytrium sp. enables the construction of a high-yielding DHA production strain. Knockout of the 2,4-dienyl-CoA reductase gene resulted in increased neutral lipids and DHA production.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Chemistry, Medicinal
Xiaohong Chen, Yaodong He, Lu Liu, Xingyu Zhu, Biswarup Sen, Guangyi Wang
Summary: This study investigated the effects of nitrogen starvation on fatty acid production in the thraustochytrid strain Aurantiochytrium sp. PKU#SW8. The results showed that nitrogen starvation increased the yields of both unsaturated and saturated fatty acids, but the increase in saturated fatty acids was disproportionately higher. The upregulation of fabD, enoyl-CoA hydratase, and pfaB genes under nitrogen starvation likely contributed to the increased fatty acid production. This study improves our understanding of the molecular mechanisms underlying the response of thraustochytrids to nitrogen deprivation.
Article
Biotechnology & Applied Microbiology
E-Ming Rau, Inga Marie Aasen, Helga Ertesvag
Summary: Thraustochytrids are marine eukaryotic microbes that produce the essential fatty acid DHA, with different genera and species producing various unsaturated fatty acids. A study found that a protein annotated as T66Des9 in a transgenic strain can synthesize specific fatty acids, suggesting a potential evolutionary link between Delta 9-desaturase and Delta 12-desaturase. This discovery provides new insights into fatty acid biosynthesis in thraustochytrids.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Vidyah Manikan, Yusuf Nazir, Aidil Abdul Hamid
Summary: Fructose has a significant positive effect on DHA content in Thraustochytrids, with a less profound impact on DHA biosynthetic capacity. When cultivated in fructose medium, Thraustochytrid species exhibit a slower growth rate but higher biomass and lipid accumulation compared to glucose medium after 48 hours.
Article
Agricultural Engineering
Nikhil L. Kadalag, Pratik R. Pawar, Gunjan Prakash
Summary: In this study, co-cultivation of Phaeodactylum tricornutum with Aurantiochytrium limacinum was used as a unique strategy to achieve the desired fatty acid composition. The initial cell density ratio was modulated to overcome the dominance of A. limacinum and regulate the proliferation of both species, resulting in the production of polyunsaturated fatty acids (PUFA) such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), as well as monounsaturated fatty acids (MUFA) and pigments.
BIORESOURCE TECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Guoxiang Chi, Yiyuan Xu, Xingyu Cao, Zhipeng Li, Mingfeng Cao, Yusuf Chisti, Ning He
Summary: Dietary consumption of omega-3 long-chain polyunsaturated fatty acids (omega-3 LC-PUFA), especially docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), has various health benefits. Traditional sources of these fatty acids, such as fish oil, are limited and of variable quality. Therefore, alternative sources are being sought. DHA derived from certain marine protists (heterotrophic thraustochytrids) has been commercially produced for high-value dietary use, but it is too expensive for use in aquaculture feeds. Aquaculture is currently dependent on wild-caught fish oil as a source of omega-3 LC-PUFA, which restricts sustainable expansion. Schizochytrium spp. (now Aurantiochytrium spp.) is of particular interest as it has shown to be a good producer of DHA and EPA. Various strategies have been developed to improve the production and economics of omega-3 PUFA in Schizochytrium spp.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Genetics & Heredity
Pranesha Prabhakaran, Nachon Raethong, Yusuf Nazir, Hafiy Halim, Wu Yang, Wanwipa Vongsangnak, Aidil Abdul Hamid, Yuanda Song
Summary: In this study, genomic characterization of Aurantiochytrium sp. SW1, a potential producer of docosahexaenoic acid (DHA), was carried out. The results reveal the genome information and metabolic pathways related to DHA synthesis in this strain. It is found that Aurantiochytrium sp. SW1 has the potential for high DHA production, and several key genes and enzymes involved in lipid metabolism and fatty acid biosynthesis pathways are identified.
Article
Fisheries
Rosana Oliveira Batista, Renata Oselame Nobrega, Delano Dias Schleder, James Eugene Pettigrew, Debora Machado Fracalossi
Summary: The research found that the addition of Aurantiochytrium sp. meal can increase the fatty acid content, especially DHA, in Nile tilapia, promoting improvements in intestinal and hepatopancreatic morphophysiology.
Article
Biochemistry & Molecular Biology
Feng-Wei Yin, Ci-Tong Zhan, Jiao Huang, Xiao-Long Sun, Long-Fei Yin, Wei-Long Zheng, Xi Luo, Ying-Ying Zhang, Yong-Qian Fu
Summary: This study investigated the effect of light intensity on the synthesis of DHA oil and carotenoids and developed a light intensity gradient strategy to improve production. The results showed that high light intensity promoted synthesis but caused cell damage, while dark culture and low light intensity conditions did not affect normal growth but hindered accumulation of carotenoids. The developed strategy significantly increased DHA and carotenoid content without reducing oil yield.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Agricultural Engineering
Ajeet Singh Chauhan, Anil Kumar Patel, Chiu-Wen Chen, Jo-Shu Chang, Philippe Michaud, Cheng-Di Dong, Reeta Rani Singhania
Summary: Considering the increasing health concerns, urban population tends to adopt a healthy lifestyle and incorporate nutritional food supplements to mitigate common health risks. The consumption of omega-3 and omega-6 PUFAs is on the rise, thus alternative commercial production methods are being developed. Microbial sources are emerging platforms to meet the global demand for omega PUFAs. Marine oleaginous protist Aurantiochytrium sp. has been identified as a potential source for substantial production of DHA and SFA. The objective of this research was to enhance PUFA yield by optimizing glucose concentration and nitrogen ratio. The maximum lipid and DHA yield and content were achieved at 30 g/L glucose, while the relative PUFA content was highest at 10 g/L glucose, with remaining SFA potentially usable for biodiesel.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Chang-Yu Wu, Tomoyo Okuda, Akinori Ando, Ayami Hatano, Hiroshi Kikukawa, Jun Ogawa
Summary: This study isolated a microorganism strain T7 from brackish areas in Japan that can efficiently produce omega 3-Docosapentaenoic acid (omega 3-DPA), a rare omega 3-polyunsaturated fatty acid (omega 3-PUFA). Strain T7, identified as a related species of Aurantiochytrium, accumulated more than 20% of total fatty acids as omega 3-DPA. The findings highlight the importance of strain T7 in investigating the physiological functions of omega 3-DPA and the metabolic pathway of fatty acids.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Agricultural Engineering
Pernille Margrethe Olsen, Gergely Kosa, Marianne Kluver, Achim Kohler, Volha Shapaval, Svein Jarle Horn
Summary: Lignocellulosic sugars from Norway spruce were used to produce docosahexaenoic acid (DHA) by the marine thraustochytrid Aurantiochytrium limacinum SR21. Shake flask batch cultivations showed that no additional salts were needed for optimal growth. Fed-batch bioreactors produced up to 55 g/L cell dry mass and a total fatty acid content of 44% (w/w), with 1/3 of it being DHA. Fourier transform infrared spectroscopy was successfully used to monitor lipid accumulation in A. limacinum SR21. This study demonstrates that crude spruce hydrolysates can be used as a sustainable resource for DHA production.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Chuanzeng Lan, Sen Wang, Huidan Zhang, Zhuojun Wang, Weijian Wan, Huan Liu, Yang Hu, Qiu Cui, Xiaojin Song
Summary: The study analyzed the functions of four DGAT2s in industrial oleaginous thraustochytrid Aurantiochytrium, with results indicating that DGAT2C mainly connects PUFA to TAG molecules, while DGAT2A and DGAT2D target SFA and/or MUFA. This research demonstrates the specific TAG assembly routes in Aurantiochytrium, providing insights for designing TAG molecules with desired characteristics.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Chemistry, Multidisciplinary
Dong Sup Kim, Han Suk Choi, Xiaoguang Yang, Ji Hyun Yang, Ja Hyun Lee, Hah Young Yoo, Jinyoung Lee, Chulhwan Park, Seung Wook Kim
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2020)
Article
Chemistry, Physical
Ji Hyun Yang, Hyeong Ryeol Kim, Ja Hyun Lee, Joon-Hyung Jin, Hee Uk Lee, Seung Wook Kim
Summary: The study aimed to enhance power output by optimizing electrical communication for GOx on a composite surface and found that immobilized GOx at a concentration of 40:80 mM EDC:NHS exhibited the highest activity with a power density of 2.24 mWcm(-2) under optimal conditions.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Biotechnology & Applied Microbiology
Hoyoung Ryu, Kyochan Kim, Hoon Cho, Eunhye Park, Yong Keun Chang, Jong-In Han
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2020)
Article
Agricultural Engineering
Joo-Young Jung, Jun Wook Hur, Kyochan Kim, Hyon-Sob Han
BIORESOURCE TECHNOLOGY
(2020)
Article
Green & Sustainable Science & Technology
Hyeong Ryeol Kim, Ju Hun Lee, Soo Kweon Lee, Youngsang Chun, Chulhwan Park, Joon-Hyung Jin, Hee Uk Lee, Seung Wook Kim
Summary: The study demonstrates the fabrication of a flexible MnO2/HNO3 pretreated biochar (NBC)/Poly(3,4-ethylenedioxythiophene) (PEDOT) composite microsupercapacitor (MSC) using pen lithography. The HNO3 pretreatment improves the dispersion of biochar ink and increases its specific surface area and graphitic carbon structure, resulting in high capacitance.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Environmental Sciences
Kang Hyun Lee, Soo Kweon Lee, Jeongho Lee, Seunghee Kim, Chulhwan Park, Seung Wook Kim, Hah Young Yoo
Summary: By pretreating chestnut shells with KOH, this study successfully increased their enzymatic digestibility and optimized reaction conditions, resulting in a significant increase in glucan content and enzymatic digestibility of untreated chestnut shells.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Article
Engineering, Chemical
Ju Hun Lee, Do Yoon Lee, Soo Kweon Lee, Hyeong Ryeol Kim, Youngsang Chun, Hah Young Yoo, Ho Seok Kwak, Chulhwan Park, Ja Hyun Lee, Seung Wook Kim
Summary: This study successfully produced 2,3-butanediol by simultaneously utilizing sugars extracted from microalgae and crude glycerol. The production yield was increased and the medium composition was optimized, contributing to the development of zero-waste processes.
Article
Engineering, Environmental
Youngsang Chun, Kyung Rae Kim, Hyeong Ryeol Kim, Soo Kweon Lee, Ju Hun Lee, Ja Hyun Lee, Chulhwan Park, Hah Young Yoo, Seung Wook Kim
Summary: The study enhanced the mechanical strength of biosorbents by using a biochar-alginate composite with a melamine sponge, analyzed the Cu(II) adsorption capacity using suitable adsorption models, and determined the maximum adsorption capacity to be 126.58 mg/g. The biosorbent was found to be effective for removing Cu(II) from wastewater.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2022)
Article
Microbiology
Kyochan Kim, Joo-Young Jung, Jong-Hee Kwon
Summary: This study evaluated the wastewater treatment capacity and feed-additive fish-growth effect of four strains of bacteria. Results showed that these strains can be effectively utilized for biological wastewater treatment processes and as aqua-feed.
Article
Environmental Sciences
Kang Hyun Lee, Soo Kweon Lee, Jeongho Lee, Seunghee Kim, Seung Wook Kim, Chulhwan Park, Hah Young Yoo
Summary: This study improved the biomass-to-glucose conversion process by optimizing the utilization of chestnut shell for bioethanol production. The energy consumption was reduced by designing an efficient pretreatment process. The ethanol yield reached 98%, suggesting the potential of chestnut shell as a feedstock for biorefinery.
ENVIRONMENTAL RESEARCH
(2022)
Article
Chemistry, Analytical
Dong Sup Kim, Xiaoguang Yang, Ja Hyun Lee, Hah Young Yoo, Chulhwan Park, Seung Wook Kim, Jinyoung Lee
Summary: Electrochemical nano-biosensors are widely used in various industries for qualitative and quantitative analyses due to their high sensitivity. This study focused on finding stable and efficient materials for real-time detection. The composite of graphite oxide/cobalt/chitosan was found to have excellent stability and electron transfer capability, and was applied in a bio-electrochemical nano-biosensor. The developed sensor successfully detected D-glucose in real-time over a wide range of concentrations, and maintained stable detection response even after 14 days.
Article
Biotechnology & Applied Microbiology
Hyeong Ryeol Kim, Kang Hyun Lee, Youngsang Chun, Soo Kweon Lee, Ju Hun Lee, Seung Wook Kim, Hah Young Yoo
Summary: Food waste-based biorefineries are important for achieving a sustainable circular economy. This study utilized cheese whey powder to produce cyclosporin A and showed the potential of using cheese whey powder as a sustainable feedstock for biorefineries.
FERMENTATION-BASEL
(2022)
Article
Polymer Science
Hye-Jin Lee, Il-Ho Choi, Seung-Wook Kim, Kyung-Ran Hwang
Summary: Al2O3 prepared by solvothermal synthesis was used as a catalyst for the catalytic dehydration of a bio-based fatty alcohol, selectively producing long-chain linear-alpha-olefins (LAO). By adjusting the synthesis temperature, amorphous alumina with the highest surface area and total pore volume was obtained, achieving efficient dehydration results.
Article
Fisheries
Joo-Young Jung, Soohwan Kim, Kyochan Kim, Bong-Joo Lee, Kang-Woong Kim, Hyon-Sob Han
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)
