Review
Medicine, Research & Experimental
John M. Macharia, Zsolt Kaposztas, Timea Varjas, Ferenc Budan, Afshin Zand, Imre Bodnar, Raposa L. Bence
Summary: Although the pathophysiology of colorectal cancer (CRC) is complex and not well understood, interactions between risk factors play a crucial role in the development and progression of the disease. The use of lactic acid bacteria (LAB) prebiotics and probiotics to modulate the tumor microenvironment (TME) has become increasingly popular in CRC management. This study aims to investigate the negative effects of LAB-derived lactic acid in the colonic mucosa in colorectal cancer management. More research is needed to ensure that LAB and their lactic acid by-products do not have unintended adverse effects in CRC management.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Materials Science, Multidisciplinary
Theeraphol Phromsopha, Yodthong Baimark
Summary: This study demonstrates that coating TPS foams with PLLA-PEG-PLLA can improve water resistance, flexibility, and phase compatibility between foam surfaces and coated films. This indicates the potential of using PLLA-PEG-PLLA coated TPS foams as starch-based foams with enhanced properties.
MATERIALS TODAY COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Vanessa Schmitt, Laura Derenbach, Katrin Ochsenreither
Summary: This study investigates the production potential of l-malic acid by the wild-type strain Aspergillus oryzae DSM 1863. Through a comparison of different process strategies, it identifies repeated-batch cultivation with product removal as the most effective method for sustained malic acid production. Additionally, the study highlights the positive effects of regular calcium carbonate additions and nitrogen supplementation on pH stability and malic acid synthesis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Polymer Science
Prasong Srihanam, Yaowalak Srisuwan, Theeraphol Phromsopha, Apirada Manphae, Yodthong Baimark
Summary: In this study, the phase compatibility and mechanical properties of flexible poly(L-lactide)-b-poly(ethylene glycol)-b-poly(L-lactide) (PLLA-PEG-PLLA)/thermoplastic starch (TPS) blends were improved by adding citric acid (CA) as a compatibilizer. The crystallinity of PLLA-PEG-PLLA matrix was suppressed and the thermal stability was enhanced by CA modification of TPS. The compatibility between TPS and PLLA-PEG-PLLA phases was improved, and the blends exhibited increased tensile strength, elongation at break, and Young's modulus with higher CA content.
Article
Polymer Science
Yaowalak Srisuwan, Yodthong Baimark
Summary: In this study, high-molecular-weight poly(L-lactide)-b-poly(ethylene glycol)-b-poly(L-lactide) triblock copolymer (PLLA-PEG-PLLA) was used as a biodegradable bioplastic. However, its high cost and poor thermal stability limited its applications. Native cassava starch was blended to reduce the production cost and improve the thermal stability. The interaction between starch and PEG increased the thermal stability and phase adhesion, resulting in flexible and hydrophilic films with potential applications in biomedical, packaging, and agricultural fields.
Article
Electrochemistry
Oleh Smutok, Taras Kavetskyy, Mykhailo Gonchar, Evgeny Katz
Summary: Lactic acid is a common metabolite in all living organisms, existing in L- and D-forms, which have various applications in biotechnology, food, pharmaceuticals, cosmetics, and the production of poly-L-lactate. Both L- and D-lactate are important biomarkers for diseases, indicators of food quality, and markers for assessing athlete training level. Enzymes such as FC b(2) and DLDH have shown high selectivity towards L- and D-stereoisomers of lactate, making them suitable for use in electrochemical biosensors.
Article
Biotechnology & Applied Microbiology
Daisuke Fukuda, Yuji Aso, Cirilo Nolasco-Hipolito
Summary: Enterococcus faecalis strain DB-5 is a newly isolated lactic acid bacterium that can produce organic acid from various carbohydrate sources. It has excellent potential for lactic acid fermentation (LAF) and can efficiently convert sucrose to lactic acid at high temperatures. These findings provide valuable information for understanding the functional properties of future high-temperature LAFs from biomass resources.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2023)
Article
Chemistry, Applied
Yaowalak Srisuwan, Yodthong Baimark
Summary: This paper reports a study on flexible polymer blends made from PLLA-PEG-PLLA and TPS. The addition of TPS suppressed crystallizability of PLLA and PLLA-PEG-PLLA matrices, while greatly increasing the thermal stability of PLLA-PEG-PLLA. The PEG middle-blocks enhanced phase compatibility between PLLA-PEG-PLLA and TPS, resulting in ductile and flexible blend films.
CARBOHYDRATE POLYMERS
(2022)
Article
Biochemical Research Methods
Kenji Okano, Yu Sato, Shnji Hama, Tsutomu Tanaka, Hideo Noda, Akihiko Kondo, Kohsuke Honda
Summary: The study demonstrates the removal of l-lactic acid from corn steep liquor and the production of optically pure d-lactic acid using an engineered Lactiplantibacillus plantarum strain expressing l-lactate oxidase. The findings have implications for the production of high-quality polylactic acid.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Agriculture, Multidisciplinary
Annalisa Ricci, Ana Belen Diaz, Camilla Lazzi, Ana Maria Blandino Garrido
Summary: This study explores the use of orange peels as a low-cost raw material for lactic acid production, eliminating the need for commercial enzymes. The enzymes produced during the fermentation of Aspergillus awamori were used for hydrolysis, and the resulting reducing sugars were fermented for lactic acid production. The preliminary results showed promising concentrations of reducing sugars and lactic acid, indicating the potential for further optimization of this approach.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Polymer Science
Alexis Theodorou, Vasilios Raptis, Chrissie Isabella Maria Baltzaki, Thrassyvoulos Manios, Vagelis Harmandaris, Kelly Velonia
Summary: In this study, we performed synthetic experiments and kinetic modeling calculations to investigate the polycondensation process of lactic acid and the formation of poly(lactic acid) (PLA). The results showed that under optimized conditions, PLLA with a weight-average molecular weight of 161 kDa could be obtained. The kinetic parameters obtained from the optimization accurately reproduced the experimental data.
Article
Food Science & Technology
Xiaolong Zhang, Zeping Liu, Bo Kang, Yao Huang, Caixia Fu, Wei Li, Qian Wu, Zhijie Liu, Dongsheng Li, Chao Wang, Ning Xu
Summary: The co-inoculation of Aspergillus oryzae with Lactobacillus plantarum or Enterococcus faecalis effectively promotes the production of active substances in soy sauce.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Ling Liu, Danlu Yang, Zhiyu Zhang, Tao Liu, Guoquan Hu, Mingxiong He, Shumiao Zhao, Nan Peng
Summary: This study developed a high-efficiency endogenous CRISPR-Cas system-based genome editing tool for Pediococcus acidilactici, allowing for various genetic manipulations. The engineered strain edited by this tool showed significant advantages in cell growth and lactic acid fermentation, making it a sophisticated chassis species for synthetic biology and bioindustry.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Materials Science, Ceramics
Guangzhi Zhang, Xiaoxia Li, Na Liu, Lina Xiong, Zhong Chen, Jiaxin Wen, Bin Zeng, Meiling Wang, Shuwang Duo
Summary: The study demonstrated that LZS QDs acted as activators to stimulate the growth of A. oryzae, significantly increasing biomass and extracellular protein content, thus potentially enhancing the production of A. oryzae.
CERAMICS INTERNATIONAL
(2022)
Article
Biotechnology & Applied Microbiology
Lihao Ji, Ju Wang, Qiuling Luo, Qiang Ding, Wenxiu Tang, Xiulai Chen, Liming Liu
Summary: The study focuses on increasing L-malate production in Aspergillus oryzae through regulating morphology engineering and fermentation processes. By screening a mutant strain (FMME-S-38) using a novel colony morphology-based system, the production of L-malate was significantly improved. The optimization of nitrogen supply strategy enhanced the productivity of L-malate, suggesting potential for industrial-scale production of similar metabolites.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Green & Sustainable Science & Technology
Aniruddha Nag, Mohammad Asif Ali, Hideo Kawaguchi, Shun Saito, Yukie Kawasaki, Shoko Miyazaki, Hirotoshi Kawamoto, Deddy Triyono Nugroho Adi, Kumiko Yoshihara, Shunsuke Masuo, Yohei Katsuyama, Akihiko Kondo, Chiaki Ogino, Naoki Takaya, Tatsuo Kaneko, Yasuo Ohnishi
Summary: The article discusses a method for producing high-temperature resistant plastics using metabolically engineered bacteria from kraft pulp, achieving significant improvement in the heat resistance of the plastic through a new organic molecular design, making an important contribution to the production of lightweight materials.
ADVANCED SUSTAINABLE SYSTEMS
(2021)
Article
Biotechnology & Applied Microbiology
Asuka Mizobata, Ryosuke Mitsui, Ryosuke Yamada, Takuya Matsumoto, Shizue Yoshihara, Hayato Tokumoto, Hiroyasu Ogino
Summary: This study developed a novel mutagenesis strategy to construct a yeast variant with high tolerance to 2,3-butanediol (2,3-BDO) by introducing simultaneous mutations into specific genes. Transcriptome analysis revealed that the yeast acquired high 2,3-BDO tolerance through amino acid function. This research provides a new approach for efficient genome modification to improve tolerance to various stressors.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Ryosuke Yamada, Yuki Kumata, Ryosuke Mitsui, Takuya Matsumoto, Hiroyasu Ogino
Summary: This study optimized the expression levels of various transcription factors to improve yeast lactic acid tolerance, leading to increased d-lactic acid production and yield under non-neutralizing conditions. Overexpression of PDR3, a transcription factor involved in multi-drug resistance, significantly enhanced lactic acid tolerance in yeast. This research highlights the potential of PDR3 as a target gene for yeast stress tolerance studies.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ryosuke Yamada, Yorichika Ando, Ryosuke Mitsui, Asuka Mizobata, Shizue Yoshihara, Hayato Tokumoto, Takuya Matsumoto, Hiroyasu Ogino
Summary: This study successfully improved the carotenoid production efficiency of recombinant S. cerevisiae using an ultrasonic-irradiation based two-phase extractive fermentation process. The use of ultrasonic waves led to an increase in the number of living cells, resulting in higher carotenoid production.
ENGINEERING IN LIFE SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Ryosuke Mitsui, Ryosuke Yamada, Takuya Matsumoto, Hiroyasu Ogino
Summary: Due to issues like the depletion of petroleum resources and price instability, the development of biorefinery technologies that utilize renewable resources to produce fuels, electric power, and chemical substances has been actively promoted. 2,3-Butanediol (2,3-BDO) is an important compound for the production of various chemicals, and recent research has focused on using biological processes, particularly involving the yeast Saccharomyces cerevisiae, for the sustainable production of 2,3-BDO from renewable resources. This review provides an overview of 2,3-BDO and its derivatives, discusses recent progress in 2,3-BDO production by microorganisms with a focus on S. cerevisiae, describes a method for separating and recovering 2,3-BDO from microbial culture medium, and outlines future prospects for industrial-scale production of 2,3-BDO by microorganisms.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Microbiology
Shin-ichi Hirano, Sota Ihara, Satoshi Wakai, Yuma Dotsuta, Kyohei Otani, Toru Kitagaki, Fumiyoshi Ueno, Akihiro Okamoto
Summary: This study identified a methanogenic isolate, Methanobacterium sp. TO1, capable of inducing iron corrosion by accepting electrons from Fe-0. Electrochemical analysis revealed that TO1 can uptake electrons at a lower potential compared to previously reported methanogens with extracellular electron transfer ability. This study highlights the corrosion risk posed by methanogens in anoxic freshwater environments and the importance of understanding the corrosion mechanism.
Article
Biotechnology & Applied Microbiology
Taiki Okamura, Yohei Nogami, Takuya Matsumoto, Ryosuke Yamada, Hiroyasu Ogino
Summary: This study aimed to improve the methanol stability of the lipase from Thermomyces lanuginosus. By using comparative biological and computational methods, the conformation of the lipase was deduced, leading to increased stability in the presence of methanol.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Microbiology
Satoshi Wakai, Nanami Eno, Hirotaka Mizukami, Toshiyuki Sunaba, Kazuhiko Miyanaga, Yasuyuki Miyano
Summary: The presence and activities of microorganisms can affect corrosion on metal surfaces. This study investigated the dynamics and differences in microbial communities on various types of stainless steels before and after corrosion incidents. The results showed that non-corroded stainless steels had slowly altered microbial communities with decreased diversity over time, while corroded stainless steels had low diversity microbial communities with enrichment of specific bacteria. The findings suggest that the composition of the stainless steel did not significantly affect the microbial communities on healthy metal surfaces.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Sota Ihara, Satoshi Wakai, Tomoko Maehara, Akihiro Okamoto
Summary: This study successfully cultured ultramicrobacteria that passed through a 0.22 μm filter from soil samples and isolated a novel species called Cellulomonas sp. strain NTE-D12. Electrochemical and genomic analyses showed that the strain NTE-D12 had a unique electron transfer system and generated the highest current density.
Article
Biotechnology & Applied Microbiology
Takuya Matsumoto, Tomoki Osawa, Hikaru Taniguchi, Akira Saito, Ryosuke Yamada, Hiroyasu Ogino
Summary: This study focused on utilizing accumulated intermediates, including acetyl-CoA, in mitochondria to enhance carotenoid production in Saccharomyces cerevisiae. By localizing carotenoid-producing enzymes in the mitochondria, the strain showed improved carotenoid production compared to the strain with enzymes in the cytosol. Furthermore, increasing glucose concentration improved cell growth and carotenoid production in mitochondria, but not in the cytosol.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Takuya Matsumoto, Kazuki Higuma, Ryosuke Yamada, Hiroyasu Ogino
Summary: In this study, the mevalonate pathway was introduced into synthetic electroactive Escherichia coli, which possesses an Mtr-based extracellular electron transfer pathway. By deleting the adhE gene and imposing a constant potential after 48 h of cultivation, mevalonate production was successfully improved. Our results provide a new perspective for the improvement of bioproduction.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemical Research Methods
Ryosuke Yamada, Moe Yokota, Takuya Matsumoto, Ben Hankamer, Hiroyasu Ogino
Summary: By co-culturing with Escherichia coli, the growth and starch content of Chlamydomonas reinhardtii were improved, likely due to the production of CO2 by E. coli promoting the growth of C. reinhardtii.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Yoshiki Yamamoto, Ryosuke Yamada, Takuya Matsumoto, Hiroyasu Ogino
Summary: Metabolic engineering of yeast Saccharomyces cerevisiae for the production of useful chemicals involves modifying gene expression. The relationship between gene expression ratio and chemical productivity is unclear, making it difficult to predict phenotypes from genotypes. This study successfully constructed a machine-learning model to clarify this relationship and predict the optimal gene expression level for efficient D-lactic acid production in yeast.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Engineering, Environmental
Chia-Lun Ho, Mohammed Y. Emran, Souta Ihara, Wenyuan Huang, Satoshi Wakai, Wei-Peng Li, Akihiro Okamoto
Summary: Labeling electrogenic bacteria from a microbiota was achieved by polymerizing 3,3'-diaminobenzidine (DAB) monomers to form DAB polymer and depositing it on the cell surface. The labeled electrogenic bacteria were then enriched through specific conjugation with magnetic nanoparticle surfaces. This rapid enrichment strategy has the potential to improve the efficiency of microbial electrochemical technologies and facilitate the identification of electrogenic bacteria.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Materials Science, Multidisciplinary
Satoshi Wakai, Nanami Eno, Kazuhiko Miyanaga, Hirotaka Mizukami, Toshiyuki Sunaba, Yasuyuki Miyano
Summary: Accelerated corrosion is frequently observed in carbon and stainless steels in freshwater environments. Immersion study revealed that microbial communities undergo significant changes during corrosion, and iron-dependent microbial energy metabolism contributes to the enrichment of other microorganisms.
NPJ MATERIALS DEGRADATION
(2022)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)
