Journal
BIORESOURCE TECHNOLOGY
Volume 146, Issue -, Pages 101-108Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.07.051
Keywords
Biochemical methane potential (BMP); Inoculum to substrate ratio (ISR); Co-digestion; Pig manure; Grass
Funding
- National Research University Project of Thailand's Office of the Higher Education Commission
- Annual Research Budget of Prince of Songkla University [ENG560080S]
- National Center of Excellence for Environmental and Hazardous Waste Management, Chulalongkorn University, Bangkok, Thailand
- Pig Business, Chareon Pokaphand Foods Public Company Limited Group, Bangkok, Thailand
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Biochemical methane potential (BMP) assay was conducted at 35 degrees C to evaluate the effects of inoculum to substrate ratio (ISR) and substrate mix ratio between para-grass and pig manure co-digesting using different inocula. Rubber latex digester (RLD) inoculum showed higher methanogenic activity (41.4 mL CH4/gVS) than pig farm digester (PFD) inoculum (37.3 mL CH4/gVS). However, the maximum methane yields, occurred at the highest para-grass mix ratio (G), were 369.6, 437.6, 465.9 and 442.6 mL CH4/gTS(added) for RLD inoculum, versus 332.4, 475.0, 519.5 and 521.9 mL/gTS(added) for PFD inoculum at ISR 1, 2, 3, and 4, respectively. HPr, HBu and HVa appeared at higher G, corresponding to substrate's higher biodegradability. Response surface indicated that higher ISR and G had a significantly positive impact on methane yield. It suggested the use of higher ISR, i.e. 3 or 4, for BMP assay of these co-substrates. Dominant species of fermentative bacteria in each inoculum was tested by DGGE. (c) 2013 Elsevier Ltd. All rights reserved.
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