Journal
BIORESOURCE TECHNOLOGY
Volume 135, Issue -, Pages 372-378Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.08.085
Keywords
Biofuel; Butanol; Clostridium; Solventogenic; Butanol dehydrogenase
Funding
- Singapore Agency for Science, Technology and Research (A*STAR) of the Science and Engineering Research Council [092 139 0034]
- Singapore National Research Foundation [NRF-CRP5-2009-05]
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A unique Clostridium species strain G117 was obtained in this study to be capable of producing dominant butanol from glucose. Butanol of 13.50 g/L was produced when culture G117 was fed with 60 g/L glucose, which is similar to 20% higher than previously reported butanol production by wild-type Clostridium acetobutylicum ATCC 824 under similar conditions. Strain G117 also distinguishes itself by generating negligible amount of ethanol, but producing butanol and acetone as biosolvent end-products. A butanol dehydrogenase gene (bdh gene) was identified in strain G117, which demonstrated a similar to 200-fold increase in transcription level measured by quantitative real-time PCR after 10 h of culture growth. The high transcription suggests that this bdh gene could be a putative gene involved in butanol production. In all, Clostridium sp. strain G117 serves as a potential candidate for industrial biobutanol production while the absence of ethanol ensures an economic-efficient separation and purification of butanol. (C) 2012 Elsevier Ltd. All rights reserved.
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