Journal
BIORESOURCE TECHNOLOGY
Volume 149, Issue -, Pages 551-555Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.09.019
Keywords
Cellobiohydrolase requirement; Ball milling pretreatment; Ionic liquid pretreatment; Cellulose crystallinity; Sugarcane bagasse
Funding
- FINEP
- JICA
- JST
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This study investigated the requirement of cellobiohydrolases (CBH) for saccharification of microcrystalline cellulose and sugarcane bagasse pretreated either by ball milling (BM) or by ionic liquid (IL) [Emim][Ac]. Hydrolysis was done using CBH-free blends of Pyrococcus horikoshii endoglucanase (EG) plus Pyrococcus furiosus beta-glucosidase (EGPh/BGPf) or Optimash (TM) BG while Acremonium Cellulase was used as control. IL-pretreated substrates were hydrolyzed more effectively by CBH-free enzymes than were the BM-pretreated substrates. IL-treatment decreased the crystallinity and increased the specific surface area (SSA), whereas BM-treatment decreased the crystallinity without increasing the SSA. The hydrolysis of IL-treated cellulose by EGPh/BGPf showed a saccharification rate of 3.92 g/L h and a glucose yield of 81% within 9 h. These results indicate the efficiency of CBH-free enzymes for the hydrolysis of IL-treated substrates. (C) 2013 Elsevier Ltd. All rights reserved.
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