Journal
BIORESOURCE TECHNOLOGY
Volume 130, Issue -, Pages 603-612Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.12.104
Keywords
Corn stover; Dilute phosphoric acid pretreatment; Response surface methodology; Recombinant Escherichia coli FBR5; Ethanol fermentation
Funding
- Turkish Council of Higher Education
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Dilute H3PO4 (0.0-2.0%, v/v) was used to pretreat corn stover (10%, w/w) for conversion to ethanol. Pretreatment conditions were optimized for temperature, acid loading, and time using central composite design. Optimal pretreatment conditions were chosen to promote sugar yields following enzymatic digestion while minimizing formation of furans, which are potent inhibitors of fermentation. The maximum glucose yield (85%) was obtained after enzymatic hydrolysis of corn stover pretreated with 0.5% (v/v) acid at 180 degrees C for 15 min while highest yield for xylose (91.4%) was observed from corn stover pretreated with 1% (v/v) acid at 160 degrees C for 10 min. About 26.4 +/- 0.1 g ethanol was produced per L by recombinant Escherichia coli strain FBR5 from 55.1 +/- 1.0 g sugars generated from enzymatically hydrolyzed corn stover (10%, w/w) pretreated under a balanced optimized condition (161.81 degrees C, 0.78% acid, 9.78 min) where only 0.4 +/- 0.0 g furfural and 0.1 +/- 0.0 hydroxylmethyl furfural were produced. (C) 2012 Elsevier Ltd. All rights reserved.
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