Journal
BIORESOURCE TECHNOLOGY
Volume 137, Issue -, Pages 286-293Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.02.050
Keywords
Co-contamination; Activated sludge; Enzyme activity; Microbial community diversity
Funding
- National Natural Science Foundation of China [41003031, 41273109]
- Shanghai Rising-Star Program [12QA1400800]
- Specialized Research Fund for the Doctoral Program of Higher Education [20110074130002]
- National Environmental Protection Public Welfare Science and Technology Research Program of China [200909089, 2010467032, 201109013]
- Key Science Foundation of Shanghai Environmental Protection Bureau [Huhuanke2012-07]
- Fundamental Research Funds for the Central Universities [WB1014004, WB1314002]
- National Science Foundation for Postdoctoral Scientists of China [2012M510811]
Ask authors/readers for more resources
This study investigated the effects of PCE and 2-CP co-contamination on growth of microbial community in terms of enzymatic activity and microbial diversity in activated sludge. Results showed that the activities of three key enzymes (dehydrogenase, phosphatase and urease) decreased significantly when PCE (in the range of 5-150 mg/L) was mixed with 2-CP (in the range of 25-150 mg/L). Especially, activity of dehydrogenase decreased by more than 93% as the concentration of PCE and 2-CP both reached 150 mg/L. PCR-DGGE revealed that short-term exposure with PCE and 2-CP did not lead to shift in the microbial community structure, while clone library demonstrated a significant change in the microbial diversity after long-term exposure. As the population of Alphaproteobacteria and Gamaproteobacteria decreased, with Actinobacteria eventually disappeared, species including Firmicutes, Bacteroidetes and Synergistetes became dominating groups. This study demonstrated that co-contamination with PCE and 2-CP affected the performance of activated sludge in a significant way. (C) 2013 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available