Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 407, Issue 23, Pages 7157-7163Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-015-8879-8
Keywords
Electrochemical immunosensor; Ara h 6; Voltammetry; Screen-printed carbon electrodes; Allergen; Peanut
Funding
- Fundacao para a Ciencia e a Tecnologia (FCT) [SFRH/BPD/68883/2010]
- POPH-QREN-Tipologia 4.1-Formacao Avancada
- FSE
- MCTES
- European Union (FEDER fund through COMPETE)
- National Fund (FCT) [UID/QUI/50006/2013]
- FEDER fund [NORTE-07-0124-FEDER-000069]
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A voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using two-monoclonal antibodies. The antibody-antigen interaction was detected through the electrochemical detection of enzymatically deposited silver. The immunosensor presented a linear range between 1 and 100 ng/ml, as well as high precision (inter-day RSD a parts per thousand currency sign9.8 %) and accuracy (recoveries a parts per thousand yen96.7 %). The detection and quantification limits were 0.27 and 0.88 ng/ml, respectively. It was possible to detect small levels of Ara h 6 in complex food matrices.
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