Journal
BIORESOURCE TECHNOLOGY
Volume 110, Issue -, Pages 546-551Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.01.040
Keywords
2-Keto-gluconic acid (2KGA); Pseudomonas fluorescens; Rice starch hydrolysate; Semi-continuous fermentation
Funding
- Research Foundation for Advanced Talents of Jiangsu University [08JDG029]
- Leaders of Disciplines and Science Cultivation Program of Jiangxi Province [2008DD00600]
- Jiangxi Provincial Engineering & Technology Research Center for Food Additives Bio-Production
- National Natural Science Foundation of China [NSFC 31101269]
- Science & Technology Program of Jiangxi Province [2010DQB00800, [2008]147]
- Science & Technology Platform Construction Program of Jiangxi Province
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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2-Keto-gluconic acid (2KGA) was produced in a semi-continuous process using Pseudomonas fluorescens AR4 and rice starch hydrolysate (RSH). The bacterium was cultured in medium with an initial glucose concentration of 170 g/L supplied as RSH. Once the glucose level had dropped to 20 g/L, 60% of the culture volume was replaced with fresh medium containing 190 g/L of glucose in the form of RSH. After an additional two cycles of growth and media replacement, a total of 476.88 g/L of glucose was consumed and 444.96 g/L of 2KGA was produced. A total productivity of 6.74 g/L and a yield of 0.93 g/g were obtained. These findings suggest that P. fluorescens AR4 is suitable for the production of commercially acceptable levels of 2KGA in semi-continuous culture. (C) 2012 Elsevier Ltd. All rights reserved.
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