Journal
BIORESOURCE TECHNOLOGY
Volume 102, Issue 5, Pages 3993-3998Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.12.062
Keywords
Non-conventional yeasts; alpha,beta-Unsaturated ketones; alpha,beta-Unsaturated aldehydes; Bioreduction; Enoate reductase
Funding
- PRIN
- Ph.D. School of Biologia Vegetale e Biotecnologie Agroambientali (University of Perugia, Italy)
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The bioreduction of alpha,beta-unsaturated ketones (ketoisophorone, 2-methyl- and 3-methyl-cyclopentenone) and aldehydes [(S)-(-)-perillaldehyde and alpha-methyl-cinnamaldehyde] by 23 non-conventional yeasts (NCYs) belonging to 21 species of the genera Candida, Cryptococcus, Debaryomyces, Hanseniaspora, Kazachstania, Kluyveromyces, Lindnera, Nakaseomyces, Vanderwaltozyma, and Wickerhamomyces was reported. The results highlight the potential of NCYs as whole-cell biocatalysts for selective biotransformation of electron-poor alkenes. A few NCYs exhibited extremely high (>90%) or even total ketoisophorone and 2-methyl-cyclopentenone bioconversion yields via asymmetric reduction of the conjugated C=C bond catalyzed by enoate reductases. Catalytic efficiency declined after switching from ketones to aldehydes. High chemoselectivity due to low competing carbonyl reductases was also sometimes observed. (C) 2010 Elsevier Ltd. All rights reserved.
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