4.8 Article

Influence of carbon source and inoculum type on anaerobic biomass adhesion on polyurethane foam in reactors fed with acid mine drainage

Journal

BIORESOURCE TECHNOLOGY
Volume 102, Issue 8, Pages 5060-5065

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.01.084

Keywords

Acid mine drainage; Sulfate; Biomass adhesion; Biofilm; Polyurethane foam

Funding

  1. FAPESP - Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [2006/06122-7, 2008/00388-0]

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This paper analyzes the influence of carbon source and inoculum origin on the dynamics of biomass adhesion to an inert support in anaerobic reactors fed with acid mine drainage. Formic acid, lactic acid and ethanol were used as carbon sources. Two different inocula were evaluated: one taken from an UASB reactor and other from the sediment of a uranium mine. The values of average colonization rates and the maximum biomass concentration (C-max) were inversely proportional to the number of carbon atoms in each substrate. The highest C-max value (0.35 g TVS g(-1) foam) was observed with formic acid and anaerobic sludge as inoculum. Maximum colonization rates (v(max)) were strongly influenced by the type of inoculum when ethanol and lactic acid were used. For both carbon sources, the use of mine sediment as inoculum resulted in a v(max) of 0.013 g TVS g(-1) foam day(-1), whereas 0.024 g TVS g(-1) foam day(-1) was achieved with anaerobic sludge. (C) 2011 Elsevier Ltd. All rights reserved.

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