Article
Environmental Sciences
Carla Fernandez-Blanco, Maria C. Veiga, Christian Kennes
Summary: This study investigates the combination of syngas fermentation and chain elongation technologies for the production of medium chain fatty acids (MCFAs) and bioalcohols. The co-culture of C. aceticum and C. kluyveri was found to metabolize syngas at near neutral pH, yielding high concentrations of n-butyrate and n-caproate. This novel system shows promise for bioproduction of MCFAs at near neutral pH, unlike previous studies which utilized acidic conditions.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Biotechnology & Applied Microbiology
Jonathan K. Otten, Yin Zou, Eleftherios T. Papoutsakis
Summary: The research demonstrates that Clostridium kluyveri can perform chain elongation to produce caproate from short chain alcohols and carboxylic acids, with significantly improved production efficiency and yield in coculture. Furthermore, exploring novel coculture partners can lead to higher caproate titers, showcasing the potential of coculture biotechnology for sustainable production of caproate and other platform chemicals.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Nebyat Seid, Katrin Ochsenreither, Anke Neumann
Summary: A novel microbial cell factory was developed to convert untreated lignocellulosic Enset fiber into caproate and H-2 by co-cultivating anaerobic fungi and bacteria. This technology provides a new value chain for Enset farmers, using locally available raw materials and low-price fermenters.
MICROBIAL CELL FACTORIES
(2023)
Article
Agricultural Engineering
Stef Ghysels, Sara Buffel, Korneel Rabaey, Frederik Ronsse, Ramon Ganigue
Summary: The study found that the addition of biochar and activated carbon in the secondary fermentation of syngas fermentation effluent can increase caproic acid production and selectivity. Biochar performs well in reducing the lag phase and increasing yield, with good recyclability. Activated carbon, especially at high doses, can enhance the conversion rate and selectivity towards caproic acid.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agricultural Engineering
He Liu, Chao Zhang, Ping Wu, Jing Li, Jie Zhang
Summary: This study compared the performances of Clostridium kluyveri, a chain elongation (CE) model strain, with or without bioaugmentation and in conventional or electro-fermentation systems. The results showed that bioaugmentation treatments, especially in electro-fermentation system, had better CE performance, with a highest caproate concentration of 4.68 g.L-1. Mechanism analysis revealed that C. kluyveri responded to the electric field and emerged synergy with the acetogens, which was supported by the increases of C. kluyveri colonization and the acetogens abundance in biofilm.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agronomy
Shota Shirane, Noriaki Momma, Toshiyuki Usami, Chiharu Suzuki, Tomoyuki Hori, Tomo Aoyagi, Seigo Amachi
Summary: Anaerobic soil disinfestation (ASD) is an ecofriendly alternative to chemical fumigation for eliminating soil-borne plant pathogens. This study found that caproate, produced by a specific strain of bacteria, is a key disinfestation factor in ethanol-based ASD, and the direct application of caproate to soil could be a promising strategy for rapid and stable soil disinfection.
Article
Agricultural Engineering
Camila A. B. Silva Rabelo, Alfonso M. Vidal, Rocio Casanova-Gonzalez, Miguel Romero-Garcia, Maria Bernadete A. Varesche, Inmaculada Romero, Eulogio Castro
Summary: This study investigates the use of vine shoots as a renewable and widely available feedstock for hydrogen production. The results showed that physical, chemical, and biological pre-treatments can enhance the hydrolysis and fermentation of vine shoots into hydrogen. Laccase pre-treatment was found to be the most effective, leading to increased production of soluble sugars and hydrogen. Additionally, the addition of cellulase during hydrolysis further improved hydrogen production. These findings have practical implications in the field of biomass energy.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biotechnology & Applied Microbiology
Maximilienne Toetie Allaart, Gerben Roelandt Stouten, Diana Z. Sousa, Robbert Kleerebezem
Summary: This study found that microbial communities enriched in Clostridium kluyveri related species were developed at pH 7.0 and 5.5 during chain elongation process using acetate and ethanol. The stoichiometry and kinetics of the process were influenced by the pH values, with different electron conversion rates and product yields observed. The results highlight the delicate balance between substrate uptake and product inhibition kinetics in chain elongating conversions.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Joongsuk Kim, Ki-Yeon Kim, Ja Kyong Ko, Sun-Mi Lee, Gyeongtaek Gong, Kyoung Heon Kim, Youngsoon Um
Summary: This study reports a newly isolated strain, Clostridium sp. JS66, which has the ability to produce C2, C4, and C6 acids/alcohols from syngas and glucose. The results demonstrate that lower agitation speed and pressure can enhance the production of C6 acids.
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2022)
Article
Environmental Sciences
Filip Brodowski, Mateusz Lezyk, Natalia Gutowska, Piotr Oleskowicz-Popiel
Summary: The study demonstrated the positive impact of external acetate on lactate-based chain elongation, leading to stable and efficient caproate production with concurrent hydrogen production. The addition of external acetate also helped maintain stable caproate production and induced previously unobserved hydrogen production even under continuous lactate overloading.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Multidisciplinary Sciences
Maximilienne Toetie Allaart, Bartholomeus B. Fox, Ingo H. M. S. Nettersheim, Martin Pabst, Diana Z. Sousa, Robbert Kleerebezem
Summary: This study demonstrates the biological feasibility of converting ethanol into hexanoate through chain elongation, bypassing the need for acetate addition and simplifying product separation. The experiments revealed the metabolic behavior of microbial communities and the role of butyrate in stabilizing the conversion process.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Jan Herzog, Alexander Mook, Tyll Utesch, Frank R. Bengelsdorf, An-Ping Zeng
Summary: Syngas fermentation processes with acetogens have the potential to reduce CO2 emissions and produce bulk chemicals. This study investigates the thermodynamic limits of acetogens in designing a fermentation process, using an anaerobic reactor and an All-in-One electrode for in-situ H-2 generation. The results show that controlling lactate production and adjusting H-2 formation using automated process control can achieve steady lactate concentrations and improve the production of medium chain fatty acids.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Agricultural Engineering
Cunsheng Zhang, Yan Liu, Wenhui Zhang, Ling Sun, Jan Baeyens
Summary: This study demonstrated that sodium hydroxide was the optimal reagent for modifying wheat straw, resulting in a 34.4% increase in specific surface area and significantly impacting caproate production. Modified wheat straw proved to be a stable matrix for cell immobilization and promoted biofilm formation, leading to efficient caproate production.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jialing Tang, Yunhui Pu, Jin Huang, Shengwang Pan, Xiaochang C. Wang, Yisong Hu, Huu Hao Ngo, Yuyou Li, Abdelfatah Abomohra
Summary: Substrate properties have a significant impact on caproate yield, and this study investigates the effect of lactate-to-acetate (LA/AA) carbon ratio and substrate loading on caproate fermentation. The optimal LA/AA carbon ratio and substrate-to-inoculums (S/I) ratio result in the highest caproate yield and selectivity.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2022)
Article
Environmental Sciences
Jingwei Ma, Yingxin Feng, Lu Li, Liang Zhu, Qiulai He, Zhou Shi, Shuizhou Ke, Qiang Ke, Quanbao Zhao
Summary: This study utilized redox mediators to improve interspecies electron transfer (IET) and enhance the production of medium chain fatty acids (MCFAs) in electro-fermentation systems. Methyl viologen (MV) promoted the conversion of acetate to ethanol, leading to increased MCFAs production. Neutral red (NR) improved chain elongation by encouraging H2 production.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Biotechnology & Applied Microbiology
Chaozhi Pan, Giin-Yu Amy Tan, Liya Ge, Chia-Lung Chen, Jing-Yuan Wang
BIOCHEMICAL ENGINEERING JOURNAL
(2016)
Article
Biotechnology & Applied Microbiology
Peixian Yang, Ling Leng, Giin-Yu Amy Tan, Chengyu Dong, Shao-Yuan Leu, Wen-Hsing Chen, Po-Heng Lee
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2018)
Article
Engineering, Environmental
Ling Leng, Masaru K. Nobu, Takashi Narihiro, Peixian Yang, Giin-Yu Amy Tan, Po-Heng Lee
Article
Environmental Sciences
Chaozhi Pan, Giin-Yu Amy Tan, Liya Ge, Chia-Lung Chen, Jing-Yuan Wang
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2019)
Article
Green & Sustainable Science & Technology
Shicong Mo, Yuhong Wang, Feng Xiong, Chunjin Ai, Dawei Wang, Giin Yu Amy Tan
JOURNAL OF CLEANER PRODUCTION
(2020)
Article
Engineering, Chemical
Minseok Kim, Theo Y. C. Lam, Giin-Yu Amy Tan, Po-Heng Lee, Jeonghwan Kim
JOURNAL OF MEMBRANE SCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Daeeun Kwon, Theo Y. C. Lam, Minseok Kim, Giin-Yu Amy Tan, Po-Heng Lee, Jeonghwan Kim
Summary: The study investigated the combined effect of ABS beads and GAC particles as fluidized media on the performance of anaerobic fluidized bed membrane bioreactor (AFMBR). The results showed that the combination of ABS and GAC improved membrane fouling efficiency and did not have adverse effects on methane production.
Review
Agricultural Engineering
Huichuan Zhuang, Po-Heng Lee, Zhuoying Wu, Houde Jing, Jianyu Guan, Xiaojing Tang, Giin-Yu Amy Tan, Shao-Yuan Leu
Summary: Anaerobic digestion (AD) is a promising technology for recovering bioenergy from biodegradable biomass, including cellulose waste. Cellulose has potential in AD but the process is substrate-specific, with cellulolysis bacteria sensitive to enzyme-substrate interactions. The cellulosome, a self-assembled enzyme complex in bacteria, is being gradually studied, but the genomic fingerprints of culture-specific cellulosome in AD are unclear, especially under processing conditions. More research is needed to clarify the factors affecting cellulosome-induced cellulolysis in AD.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Chemical
Ying Zhou, Yiang Fan, Jiliang Zhang, Changzhong Liao, Minhua Su, Ting-Shan Chan, Ying-Rui Lu, Yu-Chun Chuang, Giin-Yu Amy Tan, Kaimin Shih
Summary: The research utilized an anion intercalation strategy to functionalize polytriazine imides (PTIs) for improved heavy metal adsorption performance; The interplanar spacing was adjusted by controlling the salt matrix, temperature, and reaction time, enhancing the adsorption of lead cations (Pb2+); A new method based on the correlation between interplanar spacing and intercalated halide amounts was proposed, suggesting potential for efficient sorbent design for water purification.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Engineering, Environmental
Huichuan Zhuang, Giin-Yu Amy Tan, Houde Jing, Po-Heng Lee, Duu-Jong Lee, Shao-Yuan Leu
Summary: The pre-harvesting of organic particles through chemical enhanced primary treatment (CEPT) in sewage treatment can increase bioenergy potential and reduce energy consumption. This study provides biological proof of an acetate-methane pathway, demonstrating the potential for energy positive practice in sewage treatment.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Environmental
Ying Zhou, Changzhong Liao, Kaimin Shih, Giin-Yu Amy Tan, Minhua Su
Summary: This study evaluated the effect of anion replacement on the stability of lead in pyromorphite structure and found that isomorphous substitution decreased the durability of the products against acidic attack. The findings of this research provide important insights into the design and optimization of waste forms for heavy metal immobilization.
Article
Engineering, Environmental
Daeeun Kwon, Emma Beirns, Juhye Yoon, Theo Y. C. Lam, Giin-Yu Amy Tan, Po-Heng Lee, Jeonghwan Kim
Summary: The application of polyaniline as a coating material in anaerobic fluidized bed membrane bioreactor (AFMBR) can enhance the treatment of low-strength wastewater by reducing fouling rate and promoting methane production rate. It also activates various metabolic pathways and symbiotic relationships.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Environmental
Junseok Heo, Daeeun Kwon, Emma Beirns, Giin-Yu Amy Tan, Po-Heng Lee, Jeonghwan Kim
Summary: An anaerobic fluidized bed ceramic membrane bioreactor (AFCMBR) with polyvinylidene difluoride (PVDF) as the bio-carrier was used to treat synthetic low-strength wastewater containing high dosage of methylparaben. The study investigated the effect of hydraulic retention time (HRT) on the removal efficiency of methylparaben and the impact of methylparaben on methane content in the biogas produced. Results showed that fluidization of the PVDF biocarrier played a key role in cleaning the membrane and the fouling rate was effectively reduced with intermittent backwashing. Continuous feeding of methylparaben did not affect microbial activity, and biodegradation through biofilm formation was the dominant pathway for methylparaben removal.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Multidisciplinary Sciences
Peixian Yang, Giin-Yu Amy Tan, Muhammad Aslam, Jeonghwan Kim, Po-Heng Lee
SCIENTIFIC REPORTS
(2019)
Review
Environmental Sciences
Qian Wang, Giin-Yu Amy Tan, Mohammad Azari, Xiaowu Huang, Martin Denecke, Yujie Men, Jin-Young Jung, Satoshi Okabe, Muhammad Ali, Yu-Tzu Huang, Zhuoying Wu, Wai-hung Lo, Ji-Dong Gu, Jih-Gaw Lin, Po-Heng Lee
CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2018)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)
