Article
Multidisciplinary Sciences
Minoo Giyahchi, Hamid Moghimi
Summary: Polyester-urethanes, widely used polyurethanes, are very difficult to degrade in natural conditions. Biodegradation has become a promising approach to reduce plastic waste pollution, and in this study, two new strains of Exophilia sp. NS-7 and Rhodotorula sp. NS-12 were identified as capable of degrading polyester-polyether urethane. These strains showed positive esterase and urease activities, and could degrade -Impranil (R) within 4-6 and 8-12 days, respectively. They also exhibited PU degradation ability through the formation of pits and holes on treated films. The Sturm test and FT-IR spectrum confirmed the mineralization of PU to CO2 and changes in molecular structure, respectively, while the NMR spectrum showed destructive effects on PU films.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Chaochao Huang, Lihui Shan, Zeyu Chen, Ziliang He, Jun Li, Yang Yang, Ming Shu, Fanda Pan, Yang Jiao, Fuming Zhang, Robert J. Linhardt, Weihong Zhong
Summary: This study evaluated the differential effects of homologous NicR2A and NicR2Bs and endogenous ectopic strong promoters on nicotine metabolism in Pseudomonas sp. strain JY-Q. A feasible strategy is presented to modify wild-type (WT) strain JY-Q by removing repressing regulatory proteins NicR2A and NicR2Bs and replacing the target promoter with strong endogenous ectopic promoters. The resulting mutants exhibited high tolerance and degradation of nicotine, showing potential for enhancing the pollutant-degrading capacity of natural strains through genomic modification.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
J. Ye, S. Tian, L. Lv, Y. Ding, J. Xu, J. Zhang, L. Li
Summary: The study found that tobacco waste extract can serve as an alternative carbon source for 2-phenylethanol (2-PE) production, showing potential to increase the production yield of 2-PE with proper treatment.
LETTERS IN APPLIED MICROBIOLOGY
(2021)
Article
Environmental Sciences
Ya Wang, Zhihui Shen, Fayun Feng, Xiaolong Chen, Lixiao Song, Qun Wan, Liya Ma, Jing Ge, Jinjin Cheng, Liyun Ren, Xiangyang Yu
Summary: In this study, a Pseudomonas sp. strain F1 capable of efficiently degrading EPX was isolated from pesticide-contaminated soil. The strain showed a high capacity to degrade EPX both in soil and in vegetables, leading to a significant decrease in EPX accumulation and translocation in plants. This study demonstrates the potential of strain F1 for simultaneous bioremediation of EPX-contaminated soil and safe food production.
Article
Multidisciplinary Sciences
Francesca Mensitieri, Andrea Bosso, Fabrizio Dal Piaz, Bruno Charlier, Eugenio Notomista, Viviana Izzo, Valeria Cafaro
Summary: Livestock breeding activities and pharmaceutical wastes contribute to the accumulation of steroid hormones and estrogens in wastewaters, which have harmful effects on aquatic animals and humans. Environmental bacteria have enzymes called ring cleavage dioxygenases (RCDs) that play an important role in degrading estrogens. In this study, four extra-diol ring cleavage dioxygenases (ERCDs) were isolated and characterized. PP00124 was found to be able to efficiently degrade 4-hydroxyestradiol (4-OHE2), a carcinogenic derivative of E2, via meta cleavage. This enzyme has potential applications in bioremediation.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Kantaporn Kheawfu, Adchareeya Kaewpinta, Wisinee Chanmahasathien, Pornchai Rachtanapun, Pensak Jantrawut
Summary: The study found that nicotine extracted from the upper part of tobacco leaves using acid-base extraction method had the highest content, and the fast dissolving film formulation showed rapid and effective nicotine release.
Article
Engineering, Environmental
Yunli Liu, Haiyang Hu, Giulio Zanaroli, Ping Xu, Hongzhi Tang
Summary: The newly isolated Pseudomonas brassicacearum strain MPDS effectively degrades PAHs and heterocyclic derivatives through a unique lateral dioxygenation pathway. Genomic and transcriptomic analyses allowed for the identification of relevant gene clusters and provided new insights into the degradation mechanisms of these pollutants.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Mathematics, Applied
Lucia M. Wagner, Sara M. Clifton
Summary: Since the introduction of electronic cigarettes to the U.S. market in 2007, vaping prevalence has increased significantly in adult and adolescent populations. Concerns have been raised by the U.S. government and health professionals about the attraction of e-cigarettes to young non-smokers. A dynamical systems model was developed to analyze the competition between traditional and electronic cigarettes, predicting changes in smoking prevalence and determining the conditions under which e-cigarettes may benefit or harm society's public health.
Article
Biochemistry & Molecular Biology
Shaolin Ge, Qian Chen, Zhao Zhang, Shike She, Bingxia Xu, Fei Liu, Noor Ul Afsar
Summary: Tobacco industry generates wastewater containing inorganic mineral ions and nicotine salts, which are challenging to be treated by traditional methods. Proper techniques can regenerate tobacco stalks and use them as cigarette filler.
Article
Engineering, Environmental
Zhaoyong Xu, Tingting Zhang, Haiyang Hu, Wenzhao Liu, Ping Xu, Hongzhi Tang
Summary: This study isolated a new strain, Pseudomonas sp. NBB, capable of efficiently degrading nicotine and displaying remarkable resistance to heavy metals. The strain degraded nicotine through the pyrrolidine pathway and showed minimum inhibitory concentrations of 2 mM for barium, copper, and lead, and 5 mM for manganese. Under metal stress, the strain exhibited various strategies for tolerance, including morphological changes, up-regulation of transporters, cellular response to DNA damage, and down-regulation of ABC transporters. Notably, a manganese exporter gene (MneA) was identified among the up-regulated genes. The findings of this research provide valuable insights into the potential application of nicotine bioremediation in heavy metal-contaminated areas.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biochemical Research Methods
Jun Li, Mingjie Shen, Zeyu Chen, Fanda Pan, Yang Yang, Ming Shu, Guoqing Chen, Yang Jiao, Fuming Zhang, Robert J. Linhardt, Weihong Zhong
Summary: In this study, it was found that Nox plays a more significant role than NicA2 in nicotine degradation in TWE, showing higher thermal stability, acid stability, substrate affinity, and optimal conditions for enzyme reaction. Genetic engineering is needed to enhance the applicability of NicA2 in order to increase the synergistic effect with Nox in strain JY-Q.
PROTEIN EXPRESSION AND PURIFICATION
(2021)
Article
Biotechnology & Applied Microbiology
Shayantan Mukherji, Utpal Bakshi, Abhrajyoti Ghosh
Summary: This study reports the draft genomes of three hydrocarbon-degrading haloarchaeal strains isolated from the estuarine sediments of Sundarban mangrove forests, India. These genomes had a high GC content and encoded genes related to survival in the presence of hydrocarbons. Functional annotation revealed different pathways utilized by the strains for degrading aromatic compounds.
Article
Microbiology
Ritu Rani Archana Kujur, Subrata K. Das
Summary: This study describes a bacterium strain RBPA9 capable of degrading phenol, proposed as a novel species of Pseudomonas. The genome size is 3.92 Mb with a G+C content of 64.64%. Phylogenetic analysis revealed that strain RBPA9 clustered with Pseudomonas stutzeri ATCC 17588(T). It was found that RBPA9 expresses all the genes required in the phenol degradation pathway.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yue Yang, Ruyan Xu, Mengmeng Yang, Qiang Xu, Chenlin Miao, Jianhua Guo, Wenjun Mou, Hang Du, Gang Wei, Liwei Hu, Zongyu Hu
Summary: This study analyzed the changes in bacterial community and structural composition of tobacco leaves from flue-curing to redrying, and found that flue-curing had a greater effect on the microbial community than redrying. The relative abundance of pathway related to metabolism increased after flue-curing, while the relative abundance of pathway related to environmental information processing increased after redrying.
SCIENTIFIC REPORTS
(2023)
Article
Engineering, Environmental
Bingjun Dang, Wei Jia, Shuanglong Ma, Xiaoping Zhang, Yao Huang, Wuxing Huang, Dan Han, Kai Zhang, Fanchong Zhao, Yuwei Zhang, Zicheng Xu
Summary: In this study, a nornicotine-degrading consortium was enriched from a river sediment sample and characterized using metagenomic sequencing. Mycolicibacterium sp. strain SMGY-1XX, which has not been previously reported to degrade nornicotine, was found to be capable of degrading nornicotine, nicotine, and myosmine. The study identified candidate genes responsible for nornicotine degradation in Mycolicibacterium sp. strain SMGY-1XX through genomic, transcriptomic, and proteomic analysis. These findings provide new insights into the microbial catabolism of nornicotine and nicotine and offer potential applications for the removal, biotransformation, or detoxification of nornicotine.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)