Article
Biotechnology & Applied Microbiology
Bo-Chun Hu, Meng-Ran Li, Ying-Ying Li, Xin-Shuang Yuan, Yu-Ye Hu, Fu-Gang Xiao
Summary: In this study, a Bacillus subtilis (2,3)-butanediol dehydrogenase (BsBDHA) was engineered to enhance its catalytic activity toward (R)-phenyl1,2-ethanediol (PED) by reshaping its substrate-binding pocket (SBP) entrance. Mutagenesis experiments were performed to identify key residues and a double-site mutant, BsBDHAI49L/V266L, was obtained with significantly improved specific activity and catalytic efficiency. Molecular dynamics simulation was used to analyze the molecular mechanism of enhanced catalytic efficiency. This study provides valuable reference data for further studies.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Harshada M. Salvi, Ganapati D. Yadav
Summary: Epoxide hydrolases are enzymes widely found in nature that catalyze the hydrolysis of epoxides to diols without the need for a cofactor. A method for immobilization of epoxide hydrolase based on biomimetic mineralization has been reported, resulting in organic-inorganic hybrid nanoflowers with higher catalytic activity and stability. The nanoflowers, consisting of enzyme epoxide hydrolase and Ca2+ ions, exhibit a unique hierarchical flower-like structure and have been applied for styrene oxide hydrolysis. Factors affecting morphology, catalytic activity, and stability were studied to understand the activity recovery of the synthesized hybrid nanoflowers.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Engineering, Chemical
Chunlei Ren, Tao Wang, Xiaoyan Zhang, Jiang Pan, Jianhe Xu, Yunpeng Bai
Summary: Two novel carbonyl reductases, OdCR1 and OdCR2, were discovered and overexpressed in Escherichia coli through genome-mining approach. OdCR2 showed higher stereoselectivity compared to OdCR1, efficiently synthesizing optically pure gamma- and delta-lactones.
CHINESE JOURNAL OF CHEMICAL ENGINEERING
(2021)
Article
Computer Science, Artificial Intelligence
Yaqing Dai, Pengfei Wang, Xiaofei Zhu
Summary: Target-oriented Opinion Word Extraction (TOWE) is a subtask of Aspect Based Sentiment Analysis (ABSA) that aims to extract fine-grained opinion terms for a given aspect term from a sentence. This paper proposes a Padding-Enhanced Reinforcement Learning model (PER) that uses a multiplex heterogeneous graph and a padding module to address the long distance issue and improve the accuracy of opinion term extraction.
KNOWLEDGE-BASED SYSTEMS
(2022)
Article
Engineering, Environmental
Minakshi Gohain, Maskura Hasin, Khalifa S. H. Eldiehy, Pritam Bardhan, Khairujjaman Laskar, Hridoyjit Phukon, Manabendra Mandal, Dipul Kalita, Dhanapati Deka
Summary: This study investigated the potential of using deoiled Scenedesmus obliquus for bio-ethanol production with different heterogeneous catalysts. Successful hydrolysis of the biomass was achieved using eco-friendly catalysts, leading to the production of bio-ethanol with a high yield and concentration through fermentation.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2021)
Review
Microbiology
Nicole Robbins, Leah E. Cowen
Summary: In the past few decades, there has been a significant increase in drug-resistant fungal infections, posing a serious threat to human health. However, scientific advancements have provided hope for the discovery of new antifungal drugs. The use of chemical-genomic assays in Saccharomyces cerevisiae and molecular biology techniques in fungal pathogens has allowed for the identification and study of target genes for drug candidates, as well as the understanding of virulence attributes in diverse fungal pathogens, thus strengthening the development of antifungal drugs.
ANNUAL REVIEW OF MICROBIOLOGY
(2022)
Article
Ecology
Anjali Mahilkar, Prachitha Nagendra, Phaniendra Alugoju, E. Rajeshkannan, Supreet Saini
Summary: This study investigates the adaptive diversification of yeast in an environment containing melibiose as the carbon source. The results show that mutations in the GAL3 gene, involved in metabolic co-operation, drive the adaptive diversification. The study demonstrates the importance of metabolic co-operation with public resources in generating biological diversity.
Article
Chemistry, Multidisciplinary
Arijit Hazra, Asit Ghosh, Neeraj Yadav, Prabal Banerjee
Summary: The first asymmetric (3+3)-cycloaddition of ortho-substituted phenyl nitrones with aryl cyclopropane carbaldehydes has been achieved using secondary amine catalysts. While other ortho-substituents gave 1,2-oxazinanes, ortho-hydroxy compounds provided a novel class of tetrahydrochromeno-1,2-oxazine cores through rare 1,3-aryl migration followed by cyclization. An unusual type of asymmetric approach was also discovered.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Arijit Hazra, Asit Ghosh, Neeraj Yadav, Prabal Banerjee
Summary: The first asymmetric (3+3)-cycloaddition of ortho-substituted phenyl nitrones with aryl cyclopropane carbaldehydes has been successfully demonstrated using secondary amine catalysts. While other ortho-substituents led to 1,2-oxazinanes, ortho-hydroxy groups provided a novel class of tetrahydrochromeno-1,2-oxazine cores via rare 1,3-aryl migration followed by cyclization. An unusual type of asymmetric approach was also recognized.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Peng-sen Wang, Bo-lun Yang, Emayavaramban Perumal, Zhi-qiang Wu
Summary: In this study, a copper-supported off-Al H-Beta zeolite catalyst was prepared and used to investigate the oxidative cleavage of 1-phenyl-1,2-ethanediol. The reaction mechanism, reaction path, and kinetic parameters were studied, and the results showed that the Bronsted acid and Lewis acid had a synergistic role in the cleavage reaction. The addition of Cu species improved the acidity and acid amount of the catalyst, enhancing its CC bond breaking ability.
INTERNATIONAL JOURNAL OF CHEMICAL KINETICS
(2022)
Article
Chemistry, Inorganic & Nuclear
Mikhail A. Emelyanov, Nadezhda Stoletova, Alexander F. Smol'yakov, Mikhail M. Il'in, Victor Maleev, Vladimir A. Larionov
Summary: The study presents the synthesis of two diastereomeric cationic octahedral Co(III) complexes based on commercially available (R,R)-1,2-diphenylethylenediamine and salicylaldehyde. These complexes exhibited high selectivity and catalytic activity in challenging kinetic resolution reactions.
INORGANIC CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Ira Buntenbroich, Vincent Anton, Daniel Perez-Hernandez, Tania Simoes, Felix Gaedke, Astrid Schauss, Gunnar Dittmar, Jan Riemer, Mafalda Escobar-Henriques
Summary: Defects in mitochondrial fusion play a role in many diseases. Mitofusins use self-interaction and GTP hydrolysis to drive membrane remodeling events. The mechanisms by which mitofusins mediate outer membrane fusion are still not fully understood, but structural studies provide insights and possible therapeutic interventions for mitofusin-associated diseases.
Article
Biochemistry & Molecular Biology
Yuan Lu, Jinmei Wang, Pengpeng Cheng, Chuyue Zhang, Jinghua Li, Zhimin Ou
Summary: In this study, self-assembled dual-enzyme clusters (DENCs) were successfully prepared and their assembly was verified. DENCs showed improved storage stability and pH stability compared to free enzymes. High-yield (99.9%) and high-enantiomeric excess (99.9%) synthesis of R-BPA was achieved using DENCs, and the DENCs retained high activity after five cycles.
PROCESS BIOCHEMISTRY
(2023)
Article
Microbiology
Yasmeen N. N. Ruma, Mikhail V. V. Keniya, Brian C. C. Monk
Summary: This study characterized the functional expression of Cryptococcus CYP51 and CPR using a heterologous expression system. The expression of CYP51 increased the susceptibility to azole drugs, while co-expression of CPR decreased susceptibility to certain azole drugs.
Article
Chemistry, Multidisciplinary
Achille Antenucci, Francesco Marra, Stefano Dughera
Summary: A chiral heterogeneous catalyst proved to be efficient in a three-component asymmetric Passerini protocol conducted in a deep eutectic solvent. The reaction conditions were mild and environmentally-friendly, with excellent enantioselectivity. The catalyst was easily recovered and reused without any loss in catalytic activity.
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)