Article
Biotechnology & Applied Microbiology
Yifan Wang, Ruiyan Liu, Hong Liu, Xihai Li, Linjing Shen, Weican Zhang, Xin Song, Weifeng Liu, Xiangmei Liu, Yaohua Zhong
Summary: The synthetic hybrid promoter Pcc was constructed and applied to optimize the cellulase system of T. reesei for efficient saccharification of corncob residues. The Pcc promoter drove strong gene expression and significantly increased the activities of beta-glucosidase and endoglucanase. Moreover, the Pcc promoter allowed co-expression of two genes to enhance cellulase activity and improve saccharification efficiency towards acid-pretreated and delignified corncob residues.
MICROBIAL CELL FACTORIES
(2022)
Article
Agriculture, Multidisciplinary
Weixin Zhang, Junqi Guo, Xiaoxiao Wu, Yajing Ren, Chunyan Li, Xiangfeng Meng, Weifeng Liu
Summary: The engineered T. reesei strain showed significant differences in secreted protein composition and enzymatic activity compared to the wild-type strain, demonstrating great potential for improving saccharification efficiency of hemicellulosic constituents within corn fiber.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agricultural Engineering
Gen Zou, Dapeng Bao, Ying Wang, Sichi Zhou, Meili Xiao, Zhanshan Yang, Yinmei Wang, Zhihua Zhou
Summary: Introducing product-activated cellulase genes significantly increases enzyme activity and saccharification efficiency. Improving thermostability of cellulases was achieved through mutants and enhanced enzyme activity. Introducing product-activated cellulase genes is an effective approach to alleviate product inhibition of cellulases.
BIORESOURCE TECHNOLOGY
(2021)
Article
Microbiology
Ai-Ping Pang, Haiyan Wang, Yongsheng Luo, Zihuayuan Yang, Zhiyu Liu, Zhao Wang, Bingzhi Li, Song Yang, Zhihua Zhou, Xiaolin Lu, Fu-Gen Wu, Zuhong Lu, Fengming Lin
Summary: This study revealed that overexpression of cel3b or cel3g significantly increased extracellular beta-glucosidase production, while overexpression of cel1b inhibited cellulase production. Four cellular distribution patterns of beta-glucosidases in T. reesei were identified, providing insight into their role in cellulase production and cell function. Furthermore, the study elucidated the secretion pathways of different beta-glucosidases, advancing understanding of their properties in filamentous fungi.
Article
Biotechnology & Applied Microbiology
Tingting Long, Peng Zhang, Jingze Yu, Yushan Gao, Xiaoqin Ran, Yonghao Li
Summary: By adding miglitol to the fermentation medium of T. reesei, the cellulase titer increased more than two-fold. This method has potential in stimulating cellulase production and provides a promising system for T. reesei protein expression.
FERMENTATION-BASEL
(2022)
Article
Chemistry, Multidisciplinary
Peng Zhang, Qian Li, Yudian Chen, Nian Peng, Wenshu Liu, Xuemei Wang, Yonghao Li
Summary: This study developed a method using the byproduct of sophorose from stevioside acid hydrolysis as an inducer for cellulase production, and found that it induced cellulase production more effectively than other common soluble inducers. The crude enzyme using MGS as an inducer showed good performance in hydrolyzing corn stover. This research has the potential to reduce the production cost of inducer and contribute to improving cellulase production in industrial-scale.
Article
Biotechnology & Applied Microbiology
Su Yan, Yan Xu, Xiu-Mei Tao, Xiao-Wei Yu
Summary: It was found that knocking out vps10, vps13, and vps21 genes in T. reesei improved cellulase production by alleviating vacuolar transport and degradation. Transcriptome analysis indicated that increased cellulase gene expression might be attributed to the differential expression of sugar transporters. This study provides new clues for higher cellulase production in T. reesei through the alleviation of vacuolar transport via knockout of vps10, vps13, and vps21.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Jing Li, Yumeng Chen, Ao Gao, Liujing Wei, Dongzhi Wei, Wei Wang
Summary: A T. reesei cell factory was constructed for the first time to produce ig-carotene. By overexpressing key enzymes including CarRP, CarB, GGS1/CrtE, and HMG1, and optimizing the concentration of medium components, the modified strain achieved a titer of 218.8 mg/L of ig-carotene in flask culture. This study offers a novel and unique approach to simultaneously produce proteins and carotenoids using T. reesei industrial strains, addressing the costly waste mycelium management process.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Microbiology
Yanli Cao, Renfei Yang, Fanglin Zheng, Xiangfeng Meng, Weixin Zhang, Weifeng Liu
Summary: The ISW1 gene in Trichoderma reesei plays a crucial role in regulating cellulase and yellow pigment biosynthesis, with its deletion affecting growth and spore formation while enhancing pigment production.
Article
Microbiology
Yanli Cao, Renfei Yang, Fanglin Zheng, Xiangfeng Meng, Weixin Zhang, Weifeng Liu
Summary: This study identified a chromatin remodeling factor, TrISW1, with a dual role in coordinating the cellulolytic response and biosynthesis of the major secondary metabolite in T. reesei.
Review
Biotechnology & Applied Microbiology
Shailja Pant, Piyali Ritika, Piyali Nag, Amit Ghati, Dipjyoti Chakraborty, Mariana Rocha Maximiano, Octavio Luiz Franco, Amit Kumar Mandal, Arindam Kuila
Summary: This article mainly introduces the application of CRISPR/Cas9 technology in cellulase engineering in T. reesei. The emergence of new genetic tools over the past decade has strengthened the understanding of the transcription mechanism of cellulase genes in fungi and provided a method for editing them at the molecular level. However, the application of CRISPR technology in fungi still faces challenges and limitations.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Biotechnology & Applied Microbiology
Mukund G. Adsul, Pooja Dixit, Jitendra K. Saini, Ravi P. Gupta, Sankara Sri Venkata Ramakumar, Anshu S. Mathur
Summary: In this study, a mutant strain RC-23-1 of Trichoderma reesei RUT-C30 was obtained through mutagenesis. This mutant strain not only showed higher cellulase production but also lower viscosity during enzyme production. It has unique colony morphology and growth pattern, making it a promising candidate for large scale cellulase production.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Review
Biotechnology & Applied Microbiology
Su Yan, Yan Xu, Xiao-Wei Yu
Summary: The review summarizes recent progress in cellulase production of the filamentous fungus Trichoderma reesei, highlighting key factors such as carbon source-dependent induction, transcriptional regulation, and protein assembly. These findings shed light on potential directions for further engineering.
BIORESOURCES AND BIOPROCESSING
(2021)
Article
Agricultural Engineering
Dongmei Lv, Weixin Zhang, Xiangfeng Meng, Weifeng Liu
Summary: In order to reduce the high cost of cellulase production in lignocellulose biorefining, strategies to enhance enzyme productivity from economic and manipulatable carbon sources need to be developed. This study designed an artificial transcription factor XT by fusing the DNA binding domain of Xyr1 to the transactivation domain of Tmac1. The XT recombinant strain (OEXT) greatly improved cellulase production on repressing glucose compared with the control strain QM9414, with significant increases in pNPCase and xylanase activity.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Linjing Shen, Jia Gao, Yifan Wang, Xihai Li, Hong Liu, Yaohua Zhong
Summary: Through engineering the endoplasmic reticulum (ER) secretory pathway, the secretion of cellulolytic enzymes can be significantly increased, and the resistance to ER stress can also be regulated, thereby enhancing cellulase production.
ENZYME AND MICROBIAL TECHNOLOGY
(2022)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)