Review
Biotechnology & Applied Microbiology
Harmanpreet Kaur, Arashdeep Kaur, Sanjeev Kumar Soni, Praveen Rishi
Summary: Enzymes, known as biocatalysts, have properties like high substrate specificity and eco-friendliness, and are increasingly investigated for their anti-biofouling action in healthcare, food manufacturing, and environmental applications. Carbohydrase consortiums offer an effective alternative to conventional chemical anti-biofouling methods by targeting heterogeneous polysaccharides in the EPS matrix for complete biofilm eradication.
Article
Biochemical Research Methods
Jialiang Du, Gang Wu, Quanyao Chen, Chuanfei Yu, Gangling Xu, Anhui Liu, Lan Wang
Summary: This study used capillary isoelectric focusing to investigate the charge heterogeneity of the SARS-CoV-2 RBD. The results showed that this method can be used for the identification, evaluation of batch-to-batch consistency, and stability study of the trimeric SARS-CoV-2 RBD as part of a quality control strategy during potential vaccine production.
ANALYTICAL BIOCHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Davide Campoccia, Lucio Montanaro, Carla Renata Arciola
Summary: This article introduces the research progress on extracellular DNA (eDNA) in biofilms, highlighting the significant impact of eDNA on the pathogenesis of chronic clinical infections. The macromolecule plays a crucial role in the structural organization of bacterial biofilms through interactions with other molecular components.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Environmental Sciences
Yiying Jiao, Yongjie Zhu, Mo Chen, Liang Wan, Yijun Zhao, Jian Gao, Mingjun Liao, Xiaofang Tian
Summary: This study investigated the effects of polystyrene microplastics on the extracellular polymeric substance (EPS) produced by Microcystis aeruginosa. The results showed that high concentrations of 0.1 pm and 1.0 pm polystyrene microplastics significantly increased algal growth. All tested microplastics had the greatest inhibitory effect on photosynthesis on day 5, but the inhibition of photosynthetic activity by 0.1 pm microplastics alleviated after 13 days of exposure. The release of EPS was triggered by both sizes of microplastics at 100 mg/L concentration in the later stage of the experiment.
Article
Immunology
Richard T. Piszczatowski, Emily Schwenger, Sriram Sundaravel, Catarina M. Stein, Yang Liu, Pamela Stanley, Amit Verma, Deyou Zheng, Ronald D. Seidel, Steven C. Almo, Robert A. Townley, Hannes E. Buelow, Ulrich Steidl
Summary: Heparan sulfate (HS) glycotyping plays an important role in hematopoietic lineage differentiation and can be used to identify and stratify different cell types. By utilizing a panel of anti-HS single-chain variable fragment antibodies (scFvs), functionally distinct glycotypes within heterogeneous cell populations can be identified and further purified.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Menghe Liu, Katja Hummitzsch, Nicole A. Bastian, Monica D. Hartanti, Qianhui Wan, Helen F. Irving-Rodgers, Richard A. Anderson, Raymond J. Rodgers
Summary: During ovarian development, GREL cells and ovarian fibroblasts exhibit distinct gene expression profiles, which also differ from the cells they ultimately differentiate into.
Article
Multidisciplinary Sciences
Menghe Liu, Katja Hummitzsch, Nicole A. Bastian, Monica D. Hartanti, Qianhui Wan, Helen F. Irving-Rodgers, Richard A. Anderson, Raymond J. Rodgers
Summary: During ovarian development, GREL cells and fetal fibroblasts exhibit distinct morphological and gene expression profiles. Compared to fetal fibroblasts, GREL cells show higher expression of some genes and lower expression of others.
Article
Microbiology
Nguyen Quoc Khuong, Jakkapan Sakpirom, Truong Oanh Oanh, Le Vinh Thuc, Le Thi My Thu, Do Thi Xuan, Le Thanh Quang, Ly Ngoc Thanh Xuan
Summary: The purpose of this study was to isolate and select purple nonsulfur bacteria (PNSB) with potassium-solubilizing ability from acid paddy fields, and evaluate their ability to release plant growth-promoting substances (PGPS). A total of 70 PNSB strains were isolated from 35 acid sulfate (AS) soil samples collected in An Giang province, Vietnam. Three strains, TT07.4, AN05.1, and AC04.1, showed the highest potassium solubilization under microaerobic light and aerobic dark conditions, as well as resistance to Al3+, Fe2+, and Mn2+ toxicity. These selected strains, identified as Rhodopseudomonas pentothenatexigens, exhibited biofertilizer traits and the ability to fix nitrogen, solubilize phosphorus, produce 5-aminolevulinic acid, exopolymeric substances, indole-3-acetic acid, and siderophores. They have great potential for application in liquid or solid form to enhance rice growth and grain yield in acid sulfate soils.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Microbiology
Manoj Kamalanathan, Shawn M. Doyle, Chen Xu, Amanda M. Achberger, Terry L. Wade, Kathy Schwehr, Peter H. Santschi, Jason B. Sylvan, Antonietta Quigg
Article
Environmental Sciences
Hernando P. Bacosa, Jamie Steichen, Manoj Kamalanathan, Rachel Windham, Arnold Lubguban, Jessica M. Labonte, Karl Kaiser, David Hala, Peter H. Santschi, Antonietta Quigg
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2020)
Article
Chemistry, Multidisciplinary
Ruei-Feng Shiu, Meng-Hsuen Chiu, Carlos Vazquez, Yi-Yen Tsai, Andre Le, Agnes Kagiri, Chen Xu, Manoj Kamalanathan, Hernando P. Bacosa, Shawn M. Doyle, Jason B. Sylvan, Peter H. Santschi, Antonietta Quigg, Wei-Chun Chin
Article
Chemistry, Multidisciplinary
Luni Sun, Chen Xu, Peng Lin, Antonietta Quigg, Wei-Chun Chin, Peter H. Santschi
Summary: Photo-oxidation experiments revealed that model proteins undergo cross-linking and aggregation, becoming more resistant to proteolysis with increased carbonyl content and higher molecular weight products. The addition of reactive oxygen species accelerated these transformations.
Article
Environmental Sciences
Chi-Shuo Chen, Ruei-Feng Shiu, Yu-Ying Hsieh, Chen Xu, Carlos Vazquez, Yujia Cui, Ian C. Hsu, Antonietta Quigg, Peter H. Santschi, Wei-Chun Chin
Summary: The dynamics of organic particles in the surface ocean are crucial for the marine carbon cycle. The stickiness of extracellular polymeric substances (EPS) from different microbes plays a significant role in the aggregation and sedimentation process of particles. Factors such as salinity and surface matrices can influence the stickiness of EPS, which in turn affects the fate of organic carbon and microbial interactions in the ocean.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Microbiology
Manoj Kamalanathan, Kathleen A. Schwehr, Jessica M. Labonte, Christian Taylor, Charles Bergen, Nicole Patterson, Noah Claflin, Peter H. Santschi, Antonietta Quigg
Summary: The study found that oil exposure negatively impacted microbial substrate uptake, particularly affecting prokaryotes more rapidly and significantly. Photosynthetic activity was severely affected by oil exposure, while eukaryotic uptake of heterotrophic exopolymeric substances was less affected.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Environmental Sciences
Antonietta Quigg, Peter H. Santschi, Chen Xu, Kai Ziervogel, Manoj Kamalanathan, Wei-Chun Chin, Adrian B. Burd, Andrew Wozniak, Patrick G. Hatcher
Summary: Microbes in the ocean play a crucial role in the production of exopolymeric substances that contribute to the formation of marine snow, particularly after oil spill events. Recent experiments show rapid microbial assemblages forming on oil droplets and subsequent degradation of oil in the presence of dispersants. The protein to carbohydrate ratios of EPS serve as a chemical basis for stickiness in aggregation processes.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Environmental Sciences
Peng Lin, Chen Xu, Wei Xing, Peter H. Santschi
Summary: By utilizing molecular characterization techniques, it was revealed that there are differences in molecular structures of biopolymers derived from diatoms and coccolithophores, with diatom-derived biopolymers containing more heterogeneous elements, while coccolithophore-derived biopolymers are predominantly rich in CHO-type compounds. These distinctions in organic functional groups are crucial in understanding the behavior of 210Pb and 210Po in the oceanic ecosystem.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Environmental Sciences
Daniel Kaplan, Ralph Nichols, Chen Xu, Peng Lin, Chris Yeager, Peter H. Santschi
Summary: Recent studies in a riparian wetland in South Carolina, USA, showed strong seasonal fluctuations in groundwater radioiodine (I-129) concentrations, with higher concentrations in summer than in winter. These fluctuations were observed only in the wetlands and only with I-129, not with other contaminants. The study proposed a hypothesis that the fluctuations were a result of coupled processes involving hydrology, water temperature, microbiology, and chemistry. The findings underscore the importance of understanding the seasonal variations of contaminants for effective long-term monitoring programs in wetlands.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Chen Xu, Peng Lin, Ravindranath Garimella, Dien Li, Wei Xing, Nicole E. Patterson, Daniel Kaplan, Chris M. Yeager, Patrick G. Hatcher, Peter H. Santschi
Summary: The complex behavior of iodine isotopes and their interaction with natural organic matter pose a challenge for transport models. This study investigates the iodination of humic and fulvic acid and shows how it chemically alters the behavior of these organic matter, potentially affecting their mobility in the environment.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
W. Sanjaya Weerakkody, Ka Hin Ling, Hsueh-Han Hsieh, Vicente G. Abedneko, Jeng-Feng Shyu, Tse-Min Lee, Yung -Yen Shih, R. R. M. K. P. Ranatunga, Peter H. Santschi, Chin -Chang Hung
Summary: Rapid growth in the aquaculture industry and nutrient and organic carbon increases can cause eutrophication and greenhouse gas emissions. A novel wastewater treatment system using red macroalga, Sarcordia suae, was developed to capture CO2 in subtropical regions. Through solar powered cooling, the system efficiently removes nitrogen and phosphorus from aquaculture wastewater year-round under temperature controlled environments.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Green & Sustainable Science & Technology
Tsang-Yuh Lin, Chung-Ling Chen, Yung-Yen Shih, Hsueh-Han Hsieh, Wei-Ji Huang, Peter H. Santschi, Chin-Chang Hung
Summary: The aquaculture industry in Taiwan has a annual revenue of over USD 1.1 billion, but it also causes environmental issues such as waste discharge and groundwater withdrawals. A study in Kaohsiung, Taiwan, tested small-scale mobile cage tanks for shrimp and grouper rearing nearshore, and the results showed that the shrimp and groupers reared in these tanks had higher levels of free amino acids and were more palatable. The proposed system not only reduces the risk of eutrophication, but also has the potential for recreational fishing activities to increase income for smallholders.
Article
Engineering, Marine
Gary E. Schultz, Peter H. Santschi
Summary: Free-living bacteria in marine environments are believed to be too sparse to use density-dependent quorum sensing systems. However, this study found that marine bacteria do respond to signal molecules, indicating the presence of quorum sensing in marine gels. This discovery is significant for marine and environmental sciences.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2023)
Review
Polymer Science
Antonietta Quigg, Peter H. Santschi, Adrian Burd, Wei-Chun Chin, Manoj Kamalanathan, Chen Xu, Kai Ziervogel
Summary: Marine gels and marine snow are important in regulating global and basin-scale ocean biogeochemical cycling. Exopolymeric substances, such as transparent exopolymer particles, are abundant in the ocean and support local microbial communities. Recent studies have enhanced our understanding of these materials, but the relationship between microbial polymers and marine snow remains unclear.
Review
Polymer Science
Peter H. Santschi, Wei-Chun Chin, Antonietta Quigg, Chen Xu, Manoj Kamalanathan, Peng Lin, Ruei-Feng Shiu
Summary: Microgels play critical roles in various ocean processes, with exopolymeric substances from marine microbes being a major source. Many types of pollutants, especially hydrophobic ones, have been found to preferentially associate with marine microgels, impacting their transport and fate in the ocean. This review focuses on the interactions between gel-forming EPS and pollutants in the ocean.
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)
