Journal
BIORESOURCE TECHNOLOGY
Volume 100, Issue 19, Pages 4475-4480Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2009.04.021
Keywords
Multi-functional cellulase; Endo-beta-1,4-glucanase; Endo-beta-1,4-xylanase; Coprinus cinereus; Basidiomycete promoters
Funding
- National Natural Science Foundation of China [30371000]
- High Technology Research and Development Program of China (863 Program) [2006AA10Z301]
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Multi-functional cellulase gene infc was expressed in Coprinus cinereus under naturally non-inductive conditions using three heterologous promoters. Endo-beta-1,4-glucanase expression was achieved in solid and liquid media with promoter sequences from the Lentinula edodes gpd gene, the Flammulina velutipes gpd gene and the Volvariella volvacea gpd gene. As measured by enzyme activity in liquid cultures, a 613-bp gpd promoter fragment from L. edodes was most efficient. followed by a 752-bp gpd fragment from F. velutipes. The V. volvacea gpd promoter sequence was less active, in comparison. Irrespective of the promoter used, enzymatic activities increase 34-fold for highly active transformants and 29-fold for less active one by using cellulase-inducing medium. The highest activities of endo-beta-1,4-glucanase (34.234 U/ml) and endo-beta-1,4-xylanase (263.695 U/ml) were reached by using the L. edodes gpd promoter. (C) 2009 Elsevier Ltd. All rights reserved.
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