Article
Energy & Fuels
Maria Augusta de Carvalho Silvello, Julian Martinez, Rosana Goldbeck
Summary: This study assessed the effect of supercritical carbon dioxide (SC-CO2) on the enzymatic hydrolysis of sugarcane bagasse to produce fermentable sugars. The optimal conditions for SC-CO2 treatment were determined to be 50°C, 200 bar, and 120 min, resulting in the highest concentration of reducing sugars and hydrolysis yield.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Green & Sustainable Science & Technology
Fernando Roberto Paz-Cedeno, Lucas Ragnini Henares, Eddyn Gabriel Solorzano-Chavez, Mateus Scontri, Flavio Pereira Picheli, Ismael Ulises Miranda Roldan, Rubens Monti, Samuel Conceicao de Oliveira, Fernando Masarin
Summary: The study showed that pretreatment with sulfite-NaOH was the most efficient in removing lignin while keeping cellulose intact, and had the best response to enzymatic hydrolysis with high conversion rates.
Article
Biochemistry & Molecular Biology
Eva Balaguer Moya, Berta Syhler, Julen Ordenana Manso, Giuliano Dragone, Solange I. Mussatto
Summary: This study evaluated the optimization of enzymatic hydrolysis cocktail to maximize sugar extraction from pretreated sugarcane bagasse. It was found that adding hydrogen peroxide at the beginning of hydrolysis increased glucose and xylose concentrations, while the addition of hemicellulase increased glucose and xylose production. These findings suggest that using an appropriate enzymatic cocktail supplemented with additives can enhance sugar extraction from lignocellulosic biomass and contribute to the development of a more sustainable and competitive process.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Materials Science, Paper & Wood
Marlon Gualberto Ganter de Moura, Thiago Alessandre da Silva, Arion Zandona Filho, Marcos Lucio Corazza, Luiz Pereira Ramos
Summary: In this study, statistical design and mathematical modeling were used to investigate the fed-batch enzymatic hydrolysis process of steam-exploded sugarcane bagasse. A Box-Behnken experimental design was used to evaluate the effects of enzyme loading, stirring speed, and substrate total solids on glucose release after 48 hours of hydrolysis. A simplified kinetic model was used to fit the experimental data and predict the effects of substrate and enzyme intermittent feedings in fed-batch hydrolysis experiments. The proposed fed-batch procedure was found to significantly increase GlcEq productivity compared to batch experiments at 20 wt%.
Article
Agricultural Engineering
Rongxin Ling, Weiqi Wei, Yongcan Jin
Summary: This study explored the effect of HCl/EG-H2O pretreatment on the enzymatic conversion of cellulose in sugarcane bagasse. The results showed that the selective extraction of xylan and lignin can be achieved while maintaining high cellulose retention. The addition of enzymatic additives improved hydrolysis efficiency and reduced enzyme dosage. Furthermore, a linear relationship between substrate characteristic parameters and cellulose conversion was observed.
BIORESOURCE TECHNOLOGY
(2022)
Article
Chemistry, Physical
Wilson G. Morais Junior, Thalyta F. Pacheco, Shipeng Gao, Pedro A. Martins, Jose M. Guisan, Nidia S. Caetano
Summary: The study focused on immobilization and stabilization of two commercial enzymes for saccharification of pretreated sugarcane bagasse, showing higher thermal stability and successful immobilization of the enzymes which led to efficient cellulose hydrolysis with a solid-liquid ratio of 1:20 for 48 hours.
Article
Agricultural Engineering
Si Lu, Qiong Wang, Zheng Liang, Wen Wang, Cuiyi Liang, Zhongming Wang, Zhenhong Yuan, Ping Lan, Wei Qi
Summary: A sustainable hydrolysis method combined with catalytic pretreatment and enzymatic hydrolysis was applied to enhance reducing sugar production from sugarcane bagasse. The optimized conditions resulted in high xylose and glucose yields, as well as significantly improved enzymatic digestibility compared to natural sugarcane bagasse.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Leila Khaleghipour, Javier A. Linares-Pasten, Hamid Rashedi, Seyed Omid Ranaei Siadat, Andrius Jasilionis, Said Al-Hamimi, Roya R. R. Sardari, Eva Nordberg Karlsson
Summary: An integrated scheme was developed for successful separation of sugarcane bagasse xylan, lignin, and cellulose. Higher concentration of alkali resulted in xylan with lower degree of substitution, while high pressure conditions released more lignin than xylan. Enzymatic hydrolysis was more efficient using xylan extracted at lower alkaline strength.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Environmental Sciences
Jaspreet Kaur, Monica Sachdeva Taggar, Anu Kalia, Gulzar Singh Sanghera, Gurvinder Singh Kocher, Mohammed Javed
Summary: The study identified an efficient cellulase producing fungus and evaluated its potential for saccharification of sugarcane bagasse.
WASTE AND BIOMASS VALORIZATION
(2023)
Article
Agricultural Engineering
Xinyuan Zhang, Yiqin Wang, Jie Lu, Miaomiao Liu, Wanting Tan, Yi Cheng, Yehan Tao, Jian Du, Haisong Wang
Summary: This paper used five different types of biosurfactants to enhance the saccharification efficiency in the enzymatic hydrolysis process. The addition of sophorolipid increased the enzymatic hydrolysis efficiency by 30.65%, while the combination of sophorolipid and tea saponin further increased the efficiency to 99.56%. These biosurfactants acted synergistically to inhibit enzymatic inactivation and non-productive adsorption of lignin. This study proposes an effective method to improve enzymatic hydrolysis efficiency and reduce enzyme dosage by utilizing biosurfactants.
BIORESOURCE TECHNOLOGY
(2023)
Article
Plant Sciences
Christoph Kornpointner, Jakob Scheibelreiter, Heidi Halbwirth
Summary: Plants contain a wide range of flavonoids with varying concentrations. Hydrolysis can simplify the quantification process and improve the analysis of features unaffected by glycosylation patterns. Effective removal of glucose moieties is also crucial for the technological application of flavonoids.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agricultural Engineering
Hongdan Zhang, Wei Chen, Xueyan Han, Yibing Zeng, Jiajie Zhang, Zhennan Gao, Jun Xie
Summary: This study demonstrated that pretreatment with different metal salts, combined with Tween 80, can effectively improve the enzymatic saccharification of sugarcane bagasse, leading to higher glucose yields. The addition of Tween 80 during both pretreatment and enzymolysis processes showed superior performance in enhancing glucose yield.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Juliana P. Sandri, Julen Ordenana, Thais S. Milessi, Teresa C. Zangirolami, Solange I. Mussatto
Summary: This study focuses on a feeding strategy to enhance high solid load enzymatic hydrolysis of sugarcane bagasse and sugar co-fermentation using a non-recombinant yeast co-culture. The findings provide significant contribution to improving the feasibility of second-generation biomass energy production.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2023)
Article
Agricultural Engineering
Jun Zhang, Kuntai Li, Shucheng Liu, Shushi Huang, Chao Xu
Summary: The study optimized the AHP pretreatment conditions for sugarcane bagasse, followed by enzymatic hydrolysis at high-solids substrate loading and fed-batch fermentation to convert fermentable sugars into succinic acid (SA), achieving a high SA conversion rate of 0.29 g/g SCB raw material.
BIORESOURCE TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Olusola A. Ogunyewo, Omoaruemike E. Okereke, Sandeep Kumar, Syed Shams Yazdani
Summary: In this study, the unknown GH5 xylanase from P. funiculosum (PfXyn5) was recombinantly expressed and characterized, and its function in sugarcane bagasse (SCB) saccharification was investigated. PfXyn5 exhibited broad activity against various substrates and significantly improved the saccharification of SCB when combined with the crude cellulase cocktail of P. funiculosum. This study provides insights into the role and potential applications of PfXyn5 in the production of second-generation fuels.
SCIENTIFIC REPORTS
(2022)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)