Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 37, Issue 6, Pages 1201-1209Publisher
SPRINGER
DOI: 10.1007/s00449-013-1092-2
Keywords
Chitinase; Shrimp heads; Bacillus cereus; Affinity adsorption; PCR-DGGE
Funding
- National Science Council, Taiwan [NSC99-2313-B-032-001-MY3]
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A thermostable chitinase was purified by chitin affinity from the culture supernatant of Bacillus cereus TKU028 with shrimp head powder (SHP) as the sole carbon/nitrogen source. TKU028 chitinase was purified using a one-step affinity adsorbent system, and the molecular mass of TKU028 chitinase (approximately 40 kDa) was then determined using SDS-PAGE. The enzyme was stable for 60 min at temperatures below 60 A degrees C and stable over a broad pH range of 4-9 for 60 min. In addition, the temporal changes of a bacterial community in mangrove river sediment of the Tamsui River with added SHP were also analysed by PCR-denaturing gradient gel electrophoresis to investigate the effects of B. cereus TKU028 on the degradation of SHP. The 6-week incubation sample of SHP and B. cereus TKU028-amended mangrove river sediment displayed the highest amount of biomass, reducing sugar and total sugar, and some variance of bacterial community composition existed in the soils.
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