Article
Plant Sciences
Joseph D. Crawford, Asaph B. Cousins
Summary: The CO2-concentrating mechanism in C4 plants is initiated by the interaction between CA and PEPC, with high temperatures potentially limiting HCO3- production by CA activity but not affecting mesophyll conductance.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Agricultural Engineering
Jia-Yi Lin, Sefli Sri Wahyu Effendi, I-Son Ng
Summary: This study explores the use of genetic modification to enhance CO2 capture and utilization in microalgae for increased biomass and product production. The results show that genetically modified C. reinhardtii can achieve maximum biomass, lutein, and lipid production under autotrophic conditions in a photo-bioreactor with 5% CO2, with improved CO2 assimilation rates.
BIORESOURCE TECHNOLOGY
(2022)
Article
Chemistry, Physical
Shanshan Luo, Paul P. Lin, Liang-Yu Nieh, Guan-Bo Liao, Po-Wen Tang, Chi Chen, James C. Liao
Summary: Biological CO2 fixation is the most effective method for large-scale CO2 reduction, accounting for the majority of CO2 fixed on Earth. Cell-free CO2 fixation systems offer an alternative approach, allowing independent control of the fixation rate. By designing an oxygen-insensitive, self-replenishing CO2 fixation system with opto-sensing, the researchers achieved a sustained CO2-fixing rate comparable to or greater than photosynthetic or lithoautotrophic organisms.
Article
Agriculture, Multidisciplinary
Seung Kyou You, Hyeon Min Park, Myeong-Eun Lee, Young Jin Ko, Dong-Hyeuk Hwang, Jun Won Oh, Sung Ok Han
Summary: Metabolic engineering of non-photosynthetic microorganisms to increase the utilization of CO2 has been shown to significantly increase the production of fatty acids in the oleaginous yeast Yarrowia lipolytica. The introduction of a CO2 utilization pathway resulted in a 1.5-fold increase in fatty acid production and a 11.6% increase in linoleic acid composition compared to the wild-type strain, demonstrating the potential for developing efficient and eco-friendly processes utilizing CO2.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Plant Sciences
Jesus Perez-Lopez, Jacinto Gandullo, Clara de la Osa, Ana B. Feria, Cristina Echevarria, Jose A. Monreal, Sofia Garcia-Maurino
Summary: PPC3, a main root PEPC, plays a crucial role in responding to salinity and ammonium stress. However, Ppc3 lines showed greater susceptibility to aluminum and cadmium stress, leading to decreased growth rate and biomass production. Analysis of root exudates revealed that PPC3 is essential for the synthesis and accumulation of citrate, a key component in plant tolerance to heavy metal stress.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Review
Chemistry, Medicinal
Claudiu T. T. Supuran
Summary: Carbonic anhydrases (CAs) are widely present in organisms and play important roles in various physiological and pathological processes. Marine natural products have been valuable resources for discovering CA inhibitors, such as sulfonamides, phenols, and polyamines. Psammaplin C and bromophenols have been extensively studied as potential antitumor agents by inhibiting the activities of human CA XII and P-glycoprotein. Marine natural products offer great potential for the development of novel pharmacological agents.
Article
Chemistry, Medicinal
Gustavo Provensi, Alessia Costa, Barbara Rani, Maria Vittoria Becagli, Fabio Vaiano, Maria Beatrice Passani, Damiano Tanini, Antonella Capperucci, Simone Carradori, Jacobus P. Petzer, Anel Petzer, Daniela Vullo, Gabriele Costantino, Patrizio Blandina, Andrea Angeli, Claudiu T. Supuran
Summary: This study reported the synthesis of β-arylchalcogeno amines with structural similarity to amphetamine, which showed good activation properties for certain enzymes in the brain. In vivo evaluation demonstrated the procognitive effects of these compounds without causing unwanted side effects. These findings suggest the potential utility of these compounds for improving cognitive decline associated with neurodegenerative and psychiatric diseases.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Plant Sciences
Jesus Perez-Lopez, Ana B. Feria, Jacinto Gandullo, Clara de la Osa, Irene Jimenez-Guerrero, Cristina Echevarria, Jose A. Monreal, Sofia Garcia-Maurino
Summary: Phosphoenolpyruvate carboxylase (PEPC) has crucial functions in various processes including photosynthesis, respiration, amino acid synthesis, and seed development. Its phosphorylation by PEPC-kinase (PEPCk) is well-documented. In this study, the silencing of three sorghum genes encoding PEPCk led to decreased phosphorylation of PEPC, altered metabolic profiles and amino acid content in leaves and seeds, delayed flowering, and reduced seed quantity and quality. These findings highlight the importance of PEPC phosphorylation in sorghum development, stress responses, yield, and seed quality.
Article
Chemistry, Medicinal
Ozlem Akgul, Srishti Singh, Jacob T. Andring, Robert McKenna, Silvia Selleri, Fabrizio Carta, Andrea Angeli, Claudiu T. Supuran
Summary: A series of taurine substituted sulfonamide derivatives with ureido moiety at the tail section were synthesized as selective inhibitors of tumor associated human Carbonic Anhydrase (CA) IX and XII. These derivatives showed a strong dependence on the presence of ureido moiety and demonstrated highly stabilized ligand-protein complex with specific amino acid residues in both hCA II and hCA IX-mimic.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Environmental Sciences
David Daniel, Joao C. Campos, Paulo C. Costa, Bruno Nunes
Summary: The study evaluated the chronic ecotoxicological effects of exposure to salicylic acid and acetazolamide (alone and in combination) on marine mussel individuals, showing that ACZ inhibited CA enzyme activity at certain concentrations and significantly inhibited COX activity in the mixture, without adverse effects on shell growth and feeding behavior. Further research is needed to fully assess the effects of these pharmaceutical drugs on alternative biochemical and physiological pathways.
ENVIRONMENTAL POLLUTION
(2022)
Article
Microbiology
Guang Gao, Wei Liu, Xin Zhao, Kunshan Gao
Summary: The diatom Skeletonema costatum responds to UVR, especially UVA, by upregulating its CO2 concentrating mechanisms (CCMs) and enhancing carbon fixation activities. Additionally, UVA promotes active HCO3- uptake and photosynthetic carbon fixation, particularly at lower PAR levels.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Bingcong Ye, Zhifeng Gu, Xingzhi Zhang, Yi Yang, Aimin Wang, Chunsheng Liu
Summary: This study compared the growth, feeding, and metabolism differences between two pearl oyster species. The results showed that P. f. martensii performed better in feeding and growth, while P. maxima had higher metabolic activity.
FRONTIERS IN MARINE SCIENCE
(2022)
Review
Chemistry, Medicinal
Shubham Kumar, Sandeep Rulhania, Shalini Jaswal, Vikramdeep Monga
Summary: Carbonic anhydrase is an important enzyme involved in various physiological and pathological processes, with 16 different isoforms in humans. Inhibitors targeting different isoforms of carbonic anhydrase have clinical applications in treating various diseases, but current drugs lack selectivity leading to undesired side effects. Efforts are being made to develop novel isoform-selective inhibitors of carbonic anhydrase for therapeutic implications.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biology
Yoshihisa Hirakawa, Miki Senda, Kodai Fukuda, Hong Yang Yu, Masaki Ishida, Masafumi Taira, Kazushi Kinbara, Toshiya Senda
Summary: COG4337 proteins are a novel metal-independent variant of iota-class carbonic anhydrases, which accelerate CO2 hydration and are localized in organelles for CO2 metabolism. They share structural features with iota-class CAs but exhibit activity without reliance on metals.
Article
Biotechnology & Applied Microbiology
Jannick Kappelmann, Bianca Klein, Mathias Papenfuss, Julian Lange, Bastian Blombach, Ralf Takors, Wolfgang Wiechert, Tino Polen, Stephan Noack
Summary: The study revealed the importance of PCx and PEPCk activity for balanced carbon and energy flux in the wild-type C. glutamicum. Unexpected genotype-phenotype relationships were unraveled in the mutants through comprehensive analysis. Interesting findings included the evolution of a mutant to grow on d-glucose as the sole carbon and energy source, and compensation of prevented anaplerotic carboxylation activity by up-regulation of the glyoxylate shunt in the evolved strain.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jongrae Kim, Kwang Suk Chang, Sangmuk Lee, EonSeon Jin
Summary: The study has demonstrated the possibility of genome editing in C. vulgaris UTEX395 using CRISPR-Cas9, resulting in the generation of edited mutants nr and apt. These findings provide a foundation for further advancement in the genetics of C. vulgaris.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Julianne M. Troiano, Federico Perozeni, Raymundo Moya, Luca Zuliani, Kwangyrul Baek, EonSeon Jin, Stefano Cazzaniga, Matteo Ballottari, Gabriela S. Schlau-Cohen
Summary: The study found two distinct quenching processes, pH-dependent and zeaxanthin-dependent, within the light-harvesting complex stress-related protein LHCSR3 in green algae. These quenching processes prevent the formation of damaging reactive oxygen species and may provide different induction and recovery kinetics for photoprotection in a changing environment.
Editorial Material
Plant Sciences
Dimitris Petroutsos, Lutz Wobbe, EonSeon Jin, Matteo Ballottari
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Seo-Ree Choi, Jaewang Lee, Yeo-Jin Seo, Hyun Sun Kong, Minjae Kim, EonSeon Jin, Jung Ryeol Lee, Joon-Hwa Lee
Summary: By studying different isoforms of III antifreeze proteins, it was found that the QAE1 isoform exhibited the highest activity, while mutant constructs of the QAE2 and SP isoforms displayed similar thermal hysteresis and ice recrystallization inhibition activities compared to the QAE1 isoform. In terms of cryopreservation, the QAE1 isoform and active mutants showed better preservation of follicle morphology and more efficient prevention of DNA double-strand breaks. Additionally, active AFPs formed unique hydrogen bonds with the first 310 helix, playing a crucial role in the efficient binding to ice crystals, which was disrupted in inactive isoforms.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Hexin Lv, Minjae Kim, Seunghye Park, Kwangryul Baek, Hyeonjun Oh, Juergen E. W. Polle, EonSeon Jin
Summary: Gene expression and functional analysis of Dunaliella salina cells exposed to short-term hyperosmotic stress revealed shared and unique metabolic pathways between salt and glycerol stress conditions, providing insights for further physiological responses to osmotic shock.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Plant Sciences
Tae-Ki Park, In-A Kang, Chan-Ho Park, Jeehee Roh, Se-Hwa Lee, Minjae Kim, EonSeon Jin, Seong-Ki Kim, Tae-Wuk Kim
Summary: This study reveals that plant steroid hormone brassinosteroid regulates carotenoid content in Arabidopsis seedlings by suppressing the expression of 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE. The research also shows that this regulation differs between dark and light conditions, suggesting that BR plays a role in controlling different physiological responses in different light environments.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Biotechnology & Applied Microbiology
Inhwa Song, Sunbin Kim, Jongrae Kim, Hyeonjun Oh, Junhwan Jang, Su Jin Jeong, Kwangryul Baek, Weon-Sun Shin, Sang Jun Sim, EonSeon Jin
Summary: This study successfully generated Chlamydomonas reinhardtii mutants that are rich in both carotenoids and fatty acids by editing the AGP gene and zep mutant. These mutants can be used to produce microalgal oil enriched with macular pigments.
MICROBIAL CELL FACTORIES
(2022)
Article
Biochemistry & Molecular Biology
Bae Young Choi, Hanul Kim, Donghwan Shim, Sunghoon Jang, Yasuyo Yamaoka, Seungjun Shin, Takashi Yamano, Masataka Kajikawa, EonSeon Jin, Hideya Fukuzawa, Youngsook Lee
Summary: This study reveals how microalgae protect themselves from oxidative stress through the carbon-concentrating mechanism. The transcription factor BLZ8 was found to increase oxidative stress tolerance in microalgae and regulate the expression of carbonic anhydrase genes involved in CO2 affinity during photosynthesis. BLZ8 expression also enhanced the photosynthetic electron transfer rate and reduced reactive oxygen species production under oxidative stress conditions.
Article
Plant Sciences
Moyan Jia, Jacob Munz, Jenny Lee, Nolan Shelley, Yuan Xiong, Sunjoo Joo, EonSeon Jin, Jae-Hyeok Lee
Summary: Our study identified and characterized the bHLH factor NRI1 in Chlamydomonas, which is required to repress the use of alternative nitrogen sources. NRI1 functions as a critical repressor and coordinates diverse nitrogen starvation-induced responses, providing a crucial first step in understanding how plants and algae cope with fluctuating nitrogen availability.
Article
Biochemistry & Molecular Biology
Cassy Gerard, Regine Lebrun, Erwan Lemesle, Luisana Avilan, Kwang Suk Chang, EonSeon Jin, Frederic Carriere, Brigitte Gontero, Helene Launay
Summary: The CP12 protein is involved in the regulation of the Calvin-Benson-Bassham cycle, particularly in the dark inhibition of GAPDH and PRK enzymes. Knocking out the CP12 gene reduces PRK activity and increases the abundance of proteins involved in redox homeostasis and stress responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Sunjoo Joo, Thamali Kariyawasam, Minjae Kim, EonSeon Jin, Ursula Goodenough, Jae-Hyeok Lee
Summary: OTU2p in Chlamydomonas is a protein that prevents the degradation of organelles in plus mating-type gametes, driving uniparental organelle inheritance without gametic dimorphism.
NATURE COMMUNICATIONS
(2022)
Article
Agricultural Engineering
Byeong-ryool Jeong, Junhwan Jang, EonSeon Jin
Summary: CRISPR-Cas has revolutionized genetic modification with its simplicity and accuracy, and can be applied at the genomic level. Microalgae are excellent feedstocks for biofuels and nutraceuticals due to their high levels of fatty acids, carotenoids, and other metabolites. However, microalgal engineering is not as advanced as other model organisms. This review proposes further genome engineering schemes, including the construction of sgRNA libraries, pan-genomic and epigenomic resources, and mini-genomes, to develop synthetic biology for carbon-based engineering in microalgae.
BIORESOURCE TECHNOLOGY
(2023)
Article
Plant Sciences
Minjae Kim, Hyeon Jun Oh, Khanh Nguyen, EonSeon Jin
Summary: This study reports a newly isolated Dunaliella strain, named OH214, which can survive and grow in different concentrations of NaCl and produce high levels of carotenoids and lutein under appropriate light and nitrogen-deficient conditions. Under optimized culture conditions, this strain simultaneously produced high amounts of 6-carotene and lutein.
Article
Plant Sciences
Stefano Cazzaniga, Minjae Kim, Matteo Pivato, Federico Perozeni, Samim Sardar, Cosimo D'Andrea, EonSeon Jin, Matteo Ballottari
Summary: Targeted mutagenesis and complementation in Chlamydomonas reveal the functions of CP26, a monomeric antenna of photosystem II, demonstrating its key role in regulating photoprotection.
Article
Biotechnology & Applied Microbiology
Jun-Woo Lee, Min-Woo Lee, Chun-Zhi Jin, Hee-Mock Oh, EonSeon Jin, Hyung-Gwan Lee
Summary: This study generated a Chlamydomonas mutant with down-regulated MGD1 gene expression, which resulted in lipid remodeling and high triacylglycerol accumulation. Under high light and nitrogen starvation conditions, the mutant showed the highest productivity of neutral lipids.
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
(2022)
