Article
Plant Sciences
Yumin Shen, Mingliang Chen, Jun Hong, Wentao Xiong, Huanjin Xiong, Xiaoyan Wu, Lanxiang Hu, Yeqing Xiao
Summary: In this study, a yellow-green leaf mutant named tsyl1 was isolated and characterized in rice. The TSYL1 gene, which encodes an SRP54 protein, was found to play a key role in chlorophyll biosynthesis and its expression was affected by temperature.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Amir Akhgari, Bikash Baral, Arina Koroleva, Vilja Siitonen, David P. Fewer, Charles E. Melancon, Jani Rahkila, Mikko Metsa-Ketela
Summary: This study introduces a reporter-guided metabolic engineering tool based on random mutagenesis, selective pressure, and single-cell sorting for actinomycetes. It enables the identification of high-performing individual cells from mutant populations and has been successfully applied to strain development and drug discovery. The method allows for the activation of silent metabolic pathways and increased yields of proteins and natural products.
METABOLIC ENGINEERING
(2022)
Article
Chemistry, Analytical
Xiaohui Lin, Ping Ping Liu, Juan Yan, Donglei Luan, Tao Sun, Xiaojun Bian
Summary: Sensitive and rapid detection of pathogenic bacteria is essential. A sandwich sensor based on bacteria-imprinted polymer film (BIF) and aptamer was developed for single-cell level detection. The sensor showed high selectivity and ultra-sensitivity in detecting Staphylococcus aureus, even in complex samples. With its sensitivity, rapidity, selectivity, and cost-effectiveness, the sensor can be used as a universal platform for monitoring pathogenic bacteria in food/public safety.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Zhonglei Wang, Liyan Yang, Yake Li, Shaohua Song, Juan Qu, Rui He, Shanshan Ren, Peiwei Gong
Summary: A novel nano-system has been developed for the treatment of ALK/EGFR dual-mutant NSCLC. This nano-system addresses the concerns of low drug loading and inefficient drug permeability, providing a foundation for the development of GSH-sensitive nanoparticles for improved drug delivery.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Microbiology
Yina Wang, Keyi Wang, Amariliz Rivera, Chaoyang Xue
Summary: Invasive fungal infections, such as cryptococcosis, are difficult to treat and lack effective vaccines. However, heat-killed fbp1 Delta cells (HK-fbp1) have shown potential as a vaccine candidate, providing immunity against lethal fungal pathogens. This study revealed that T cells, specifically CD4(+) or CD8(+) T cells, are critical for the vaccine-induced protection. Additionally, HK-fbp1 administration can inhibit disease dissemination when used as a therapeutic agent at the early stage of infection.
MICROBIOLOGY SPECTRUM
(2023)
Article
Engineering, Chemical
Victor Berned-Samatan, Alejandro Galan-Gonzalez, Edgar Munoz, Ana M. M. Benito, Wolfgang K. K. Maser, Joaquin Coronas, Carlos Tellez, Cesar Rubio
Summary: The development of membranes for nanofiltration applications requires selective layers and suitable supports. This study explores the use of carbon nanotube films as supports for polyamide layers and the preparation of a double layer polyamide/zeolitic imidazolate framework. The results show promising membrane performance for selective separation processes.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Engineering, Environmental
Nadeem Baig, Asif. Matin, M. Faizan, Deepak Anand, Irshad Ahmad, Safyan A. Khan
Summary: This study reports a surface modification of ultrafiltration membranes with a loose layer of nanosilver immobilized polydopamine, which can effectively be used for fractionating dyes and divalent salts in textile wastewater.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Chemistry, Medicinal
Emily J. Hanan, Marie-Gabrielle Braun, Robert A. Heald, Calum MacLeod, Connie Chan, Saundra Clausen, Kyle A. Edgar, Charles Eigenbrot, Richard Elliott, Nicholas Endres, Lori S. Friedman, Emily Gogol, Xiao-Hui Gu, Rebecca Hong Thibodeau, Philip S. Jackson, James R. Kiefer, Jamie D. Knight, Michelle Nannini, Raman Narukulla, Amanda Pace, Jodie Pang, Hans E. Purkey, Laurent Salphati, Deepak Sampath, Stephen Schmidt, Steve Sideris, Kyung Song, Swathi Sujatha-Bhaskar, Mark Ultsch, Heidi Wallweber, Jianfeng Xin, SiewKuen Yeap, Amy Young, Yu Zhong, Steven T. Staben
Summary: This study optimized a series of benzoxazepin-oxazolidinone ATP-competitive inhibitors of PI3K alpha that also induce selective degradation of the mutant p110 alpha protein. Structure-based design led to potent inhibitors with over 300-fold selectivity and further optimization of pharmacokinetic properties resulted in excellent in vivo efficacy. Clinical candidate GDC-0077 (inavolisib) is now being evaluated in a Phase III clinical trial for treating patients with PIK3CA-mutant breast cancer.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Shashi Sharma, Paban Kumar Dash, Sushil Kumar Sharma, Ambuj Srivastava, Jyoti S. Kumar, B. S. Karothia, K. T. Chelvam, Sandeep Singh, Abhaydeep Gupta, Ram Govind Yadav, Ruchi Yadav, T. S. Greeshma, Pramod Kumar Kushwaha, Ravi Bhushan Kumar, D. P. Nagar, Manvendra Nandan, Subodh Kumar, Duraipandian Thavaselvam, Devendra Kumar Dubey
Summary: Genome sequence analysis sheds light on the transmission patterns and impact of COVID-19 in the central Indian state of Madhya Pradesh. Scientists identified multiple virus variants and traced the introduction and evolution processes within the region during the pandemic response.
SCIENTIFIC REPORTS
(2021)
Article
Biophysics
Tanmay Chatterjee, Alexander Johnson-Buck, Nils G. Walter
Summary: Sensitive assays of protein biomarkers play a critical role in clinical diagnostics and biomedical research. Nucleic acid aptamers generated by SELEX have shown promise as an alternative to immunoreagents for detection, offering advantages in synthesis and reproducibility. SiMREPS, a single molecule recognition platform, allows for high sensitivity detection of biomarkers without requiring high-affinity probes. This study demonstrates the applicability of aptamers as detection probes in SiMREPS for two clinically relevant biomarkers, VEGF165 and IL-8, with detection limits in the low femtomolar range.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Physical
Bixia Liang, Kaixin Chen, Haoling Chen, Wenkang Liu, Xingyu An, Jin Quan, Zongxia Mou, Ying Ma, Ping Zhou, Nan Li, Xin Cui
Summary: A sensitive and robust single-particle SERS sensing platform was developed for profiling Staphylococcus aureus (SA) in complex samples. The platform utilized hierarchical Au microparticles (HAMs) for capturing SA, followed by sandwiching the captured SA with aptamer-modified spherical Au nanoparticles (AuNPs) to enhance SERS signals. The method showed a broad linear range of SA detection and successfully detected SA in milk samples.
APPLIED SURFACE SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Aipeng Li, Ting Wang, Qing Tian, Xiaohong Yang, Dongming Yin, Yong Qin, Lianbing Zhang
Summary: The study focused on the control of enzyme stereoselectivity, successfully designing the variant Q139G which switched stereoselectivity without compromising activity. The mutant showed preference towards antiPrelog with beta-ketoesters, while not leading to reversed stereoselectivity with aromatic ketones.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Multidisciplinary Sciences
Guodong Yuan, Felipe Curtolo, Yibing Deng, Tao Wu, Fang Tian, Qun Ma, Yutong Liu, Jinglin Zuo, Guilherme Menegon Arantes, Peng Zheng
Summary: MT is a metal-bound protein that plays essential roles in metal homeostasis and heavy metal detoxification. It contains dynamic polynuclear metal clusters with multiple conformational states, contributing to its diverse cellular functions as a metal-carrier protein.
Article
Multidisciplinary Sciences
Fanjie Meng, Janghyun Yoo, Hoi Sung Chung
Summary: In this study, a method combining single-molecule fluorescence lifetime imaging and deep learning was developed to monitor individual fibril formation in real time and analyze them in a high-throughput manner. This method allows for quantitative characterization of highly heterogeneous fibril formation and the discovery of a common nucleation mechanism.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Virology
Kathleen K. M. Glover, Danica M. Sutherland, Terence S. Dermody, Kevin M. Coombs
Summary: Temperature-sensitive MRV mutants tsG453 and tsC447 were characterized to identify the dominant amino acid substitutions responsible for their respective phenotypes, with Asn(16)-Lys identified as solely responsible for the tsG453 phenotype. The study highlights the utility of reverse genetics in delineating structure-function relationships and provides a basis for further studies on MRV mutants.