Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 33, Issue 2, Pages 179-185Publisher
SPRINGER
DOI: 10.1007/s00449-009-0302-4
Keywords
Amperometric immunosensor; Carcinoembryonic antigen; Ions derivation; Prussian blue; Nanogold
Funding
- National Natural Science Foundation of China [20675064]
- Chinese Education Ministry Foundation for Excellent Young Teachers [2002-40]
- National Science Foundation of Chongqing City [CSTC-2004BB4149, 2005BB4100]
- High Technology Project Foundation of Southwest University [XSGX 02]
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[AuCl4](-) was initially deposited by electrochemical reduction on a glassy carbon electrode (GCE) to form porous nanogold layer, then prussian blue (PB) was electrodeposited onto the as-prepared nanogold layer, and then secondary nanogold particles were fabricated again on the PB surface by electrochemical reduction for the immobilization of anti-CEA antibodies. The presence of double-layer porous gold nanoparticles enhanced the immobilized amount of biomolecules, and improved the sensitivity of the immunoassay. PB, as a good redox probe, was facile to electrochemical analysis and measurement. Under optimal conditions, the developed immunoassay exhibited dynamic range from 3.0 to 80.0 ng/mL with a detection limit of 0.9 ng/mL CEA (S/N = 3). Moreover, the selectivity, reproducibility and stability of the immunosensor were acceptable.
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