Article
Chemistry, Multidisciplinary
Shuang Chen, Yongzhuo Liu, Ri Liang, Gaobo Hong, Jing An, Xiaojun Peng, Wen-Heng Zheng, Fengling Song
Summary: Novel acid-activatable self-assembled nanophotosensitizers based on an amphiphilic peptide derivative have been developed, showing specific tumor cell entry and disassembly inside lysosomes, with fluorescence turn-on and photodynamic therapy effect. These smart nanophotosensitizers offer new opportunities for cancer theranostics.
CHINESE CHEMICAL LETTERS
(2021)
Article
Chemistry, Organic
Shakti K. Maurya, Sushil Swaroop Pathak, Leela S. Panchakarla, Harkesh B. Singh
Summary: In this study, Fc conjugated selenopeptides were synthesized using a modified SPSS protocol, and their self-assembled nanostructures were investigated. The importance of the Fc unit, aromatic moieties, and weak intermolecular interactions on the self-assembled process was revealed through systematic morphological studies.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Polymer Science
James P. Warren, Matthew P. Culbert, Danielle E. Miles, Steven Maude, Ruth K. Wilcox, Paul A. Beales
Summary: Self-assembling peptides are a versatile biomaterial that can form self-supporting hydrogels. Balancing attractive and repulsive forces through peptide charge and hydrogen bonding is crucial for successful hydrogel formation. A net peptide charge of +/-2 and modulating hydrogen bonding can optimize the assembly and viscoelastic properties of the hydrogel. These findings demonstrate the potential of controlling self-assembly mechanisms to create structures with desired properties.
Article
Engineering, Biomedical
Yichen Yuan, Yejiao Shi, Jayati Banerjee, Amin Sadeghpour, Helena S. Azevedo
Summary: The use of synthetic extracellular matrices (ECMs) has been important in investigating the interaction between cells and matrix components in cell culture studies. To provide cells with a more natural environment, designing matrices that mimic the composition and structure of natural ECMs is desirable. This study presents the supramolecular fabrication of peptide-hyaluronan (HA) hydrogels as potential ECM substitutes, which combine native HA and rationally designed cationic amphipatic peptides.
MATERIALS TODAY BIO
(2023)
Review
Pharmacology & Pharmacy
Xiaosong Le, Tianwen Gao, Li Wang, Feng Wei, Cuixia Chen, Yurong Zhao
Summary: A variety of functional biomaterials with different sizes and morphologies can be created through self-assembly, with a particular focus on amphiphilic peptide-based materials. These peptides can self-assemble into diverse nanostructures, and their formation is governed by a delicate balance of non-covalent interactions. This review highlights recent advances in the self-assembly of two types of peptides, surfactant-like peptides (SLPs) and peptide amphiphiles (PAs), and discusses their applications in various biomedical fields.
CURRENT PHARMACEUTICAL DESIGN
(2022)
Article
Biochemistry & Molecular Biology
Alessandra Scelsi, Brigida Bochicchio, Andrew M. Smith, Antonio Laezza, Alberto Saiani, Antonietta Pepe
Summary: The assembly of gelating peptides with elastin-inspired peptide or dipeptide was investigated, revealing different nanotopologies and mechanical properties that could be utilized for fabricating materials with desired properties.
Article
Food Science & Technology
J. Mildenberger, M. Remm, M. Atanassova
Summary: Hydrogels based on marine natural biopolymers, such as peptide fractions from Parastichopus tremulus, have been characterized for their antioxidant activity and self-assembly capacity. The study identified potential peptide sequences with auto-assembly capacity, providing new materials for tissue engineering in regenerative medicine.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marina Kurbasic, Ana M. Garcia, Simone Viada, Silvia Marchesan
Summary: The study focused on the catalytic effects of bioactive hydrogels based on tripeptide self-assembly, specifically exploring the impact of modifications on the hydrogel and esterase mimicry activity.
The synthesis and characterization of three analogues with terminal modifications were analyzed, and their self-assembly, hydrogelation, and esterase-like biocatalysis were tested through various techniques.
These findings offer valuable insights into how chemical modifications affect the self-assembly of biocatalytic hydrogels and can potentially aid in the design of supramolecular catalysts for enhanced performance in the future.
Article
Biochemistry & Molecular Biology
Naoki Tsutsumi, Akitaka Ito, Azumi Ishigamori, Masato Ikeda, Masayuki Izumi, Rika Ochi
Summary: In this study, a novel bola-amphiphilic glycosylated lipopeptide-type supramolecular hydrogelator is presented, exhibiting reversible thermochromism and gel-sol transition. The investigation focused on the effects of different numbers of F residues on gelation ability and morphology of self-assembled nanostructures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Giacomo Fabrini, Aisling Minard, Ryan A. Brady, Marco Di Antonio, Lorenzo Di Michele
Summary: In this study, responsive hydrogel particles were prepared by combining noncanonical DNA G-quadruplex structures with amphiphilic DNA constructs. These particles are capable of responding to physiological stimuli in vivo and can trigger and control assembly/disassembly by adding or removing ions. Additionally, the aggregates can be photo-disassembled in the presence of a photosensitizer.
Article
Chemistry, Multidisciplinary
Priscila Cardoso, Samuel Appiah Danso, Andrew Hung, Chaitali Dekiwadia, Nimish Pradhan, Jamie Strachan, Brody McDonald, Kate Firipis, Jacinta F. White, Arturo Aburto-Medina, Charlotte E. Conn, Celine Valery
Summary: Microbial resistance to antibiotics is becoming a major crisis, and antimicrobial peptides (AMPs) are being explored as an alternative. This study reports the design of Fmoc-peptides with dual functionality, as antimicrobial agents and hydrogelators. Molecular dynamics simulations predicted the self-assembly of Priscilicidin into nanostructures in water, and experimental results confirmed its antimicrobial activity and hydrogel-forming capacity, including against multidrug resistant strains.
FRONTIERS IN CHEMISTRY
(2023)
Article
Materials Science, Multidisciplinary
Saikat Chakraborty, Christian M. Berac, Moritz Urschbach, Daniel Spitzer, Markus Mezger, Pol Besenius, Thomas Speck
Summary: This study investigates the morphologies and assembly kinetics of pH-responsive supramolecular polymers and develops a model to reproduce the experimental observations. The research finds that acidic and basic peptides in aqueous solution exhibit opposite aggregation states at extreme pH conditions and undergo homo-to-copolymer transition in equimolar mixtures.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Ottavia Bellotto, Slavko Kralj, Michele Melchionna, Paolo Pengo, Matic Kisovec, Marjetka Podobnik, Rita De Zorzi, Silvia Marchesan
Summary: This study investigated the self-assembling behavior of distinct stereoisomers of Ile-Phe and Phe-Ile in PBS to form hydrogels, confirming peptide purity and identity through NMR, mass spectrometry, and crystalline XRD data. One stereoisomer was found to exhibit fast kinetics and the highest elastic modulus, making it the only gelator in the study to self-organize into stable hydrogels with distinctive features.
Article
Chemistry, Multidisciplinary
Tomoyuki Koga, Aika Ikejiri, Nobuyuki Higashi
Summary: In this study, the hierarchical self-assembly and self-sorting of collagen-inspired peptides (CPs) were described. The CPs formed triple helix structures when cooled, which further self-assembled into highly ordered vesicles. Interestingly, the CPs exhibited narcissistic self-sorting behavior during the formation of both triple helix and matured vesicles. Due to the difference in triple helix formation at different temperatures, the CPs discriminated each other and formed distinct foldamers. These findings provide important insights for the design of complex and ordered peptide self-assembly systems with potential applications in nanobiotechnology.
Article
Multidisciplinary Sciences
Ashley K. Nguyen, Thomas G. Molley, Egi Kardia, Sylvia Ganda, Sudip Chakraborty, Sharon L. Wong, Juanfang Ruan, Bethany E. Yee, Jitendra Mata, Abhishek Vijayan, Naresh Kumar, Richard D. Tilley, Shafagh A. Waters, Kristopher A. Kilian
Summary: This article reports the discovery of a short peptide based on the tryptophan zipper motif, which shows multiscale hierarchical ordering and displays antimicrobial and self-healing properties. It can be used for cell delivery and bioprinting, and has important implications for supramolecular assembly of soft materials in biotechnology and medicine.
NATURE COMMUNICATIONS
(2023)
Article
Biophysics
Ifat Cohen-Erez, Hanna Rapaport
COLLOIDS AND SURFACES B-BIOINTERFACES
(2018)
Article
Chemistry, Physical
Avigail Baruch Leshem, Sivan Isaacs, Sachin K. Srivastava, Ibrahim Abdulhalim, Ariel Kushmaro, Hanna Rapaport
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2018)
Article
Chemistry, Multidisciplinary
Hodaya Green, Guy Ochbaum, Anna Gitelman-Povimonsky, Ronit Bitton, Hanna Rapaport
Article
Polymer Science
Ifat Cohen-Erez, Noa Harduf, Hanna Rapaport
POLYMERS FOR ADVANCED TECHNOLOGIES
(2019)
Article
Nanoscience & Nanotechnology
Ifat Cohen-Erez, Carol Issacson, Yael Lavi, Ruthy Shaco-Levy, Jammi Milam, Brenda Laster, Levi A. Gheber, Hanna Rapaport
ACS APPLIED MATERIALS & INTERFACES
(2019)
Article
Chemistry, Physical
Elad Arad, Hodaya Green, Raz Jelinek, Hanna Rapaport
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2020)
Article
Engineering, Biomedical
Ziv Azoulay, Polina Aibinder, Ayala Gancz, Jacob Moran-Gilad, Shiri Navon-Venezia, Hanna Rapaport
Summary: FKF, a tripeptide with potential antimicrobial activity, forms a pure peptide hydrogel phase made of natural amino acids. The hydrogel exhibits bactericidal activity against E. coli and P. aeruginosa, and reduces P. aeruginosa contamination in a rat skin lesion model.
ACTA BIOMATERIALIA
(2021)
Article
Surgery
Fadi Heno, Ziv Azoulay, Boris Khalfin, Hillary A. Craddock, Eldad Silberstein, Jacob Moran-Gilad, Hanna Rapaport
Summary: The development of a biomaterial coating chemically bonded to silicone implants that releases antimicrobial ions over time shows promising antibacterial effects against common etiological agents of breast implant infections, with continued efficacy observed up to 48 hours.
AESTHETIC PLASTIC SURGERY
(2021)
Article
Biochemistry & Molecular Biology
May Meltzer, Noam Eliash, Ziv Azoulay, Uzi Hadad, Niv Papo
Summary: The study focuses on the importance of Tie1 and Tie2 receptor tyrosine kinases in various diseases, especially cancer. Using non-immune nanobodies, the researchers discovered a highly expressive and specific nanobody that binds to Tie1, with potential therapeutic effects on cancer.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Polymer Science
Topaz Levi, Gal Yosefi, Ronit Bitton, Hanna Rapaport
Summary: This article presents the development of hierarchically ordered planar and spherical macroscopic membranes constructed by an amphiphilic and cationic beta-sheet peptide that spontaneously assembles with the negatively charged alginate biopolymer. The suitability of these membranes as substrates for cell culture applications was examined, and the results demonstrate their stability and potential use in studying cancer cells and cell therapies.
POLYMERS FOR ADVANCED TECHNOLOGIES
(2022)
Article
Chemistry, Multidisciplinary
Elad Arad, Gal Yosefi, Sofiya Kolusheva, Ronit Bitton, Hanna Rapaport, Raz Jelinek
Summary: Glucagon amyloid fibrils have been found to catalyze important biological reactions, including dephosphorylation of ATP. The catalytic activity is located in an enzymatic pocket-like triad formed on the surface of the glucagon fibrils.
Article
Materials Science, Biomaterials
Shahar Dahan, Polina Aibinder, Boris Khalfin, Jacob Moran-Gilad, Hanna Rapaport
Summary: The resistance of pathogenic bacteria to conventional antibiotics has led to the development of new antimicrobial peptides (AMPs), which can be used as topical treatments for skin infection and wound regeneration. In this study, FKF hydrogels were combined with gelatin to improve their biocompatibility. The hybrid hydrogels showed enhanced antimicrobial activity and could serve as scaffolds for tissue regeneration.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Polymer Science
Ifat Cohen-Erez, Noa Harduf, Hanna Rapaport
POLYMERS FOR ADVANCED TECHNOLOGIES
(2019)
Article
Pharmacology & Pharmacy
Elad Arad, Susanta Kumar Bhunia, Jurgen Jopp, Sofiya Kolusheva, Hanna Rapaport, Raz Jelinek
ADVANCED THERAPEUTICS
(2018)
Article
Biochemistry & Molecular Biology
Elad Arad, Ravit Malishev, Hanna Rapaport, Raz Jelinek