Journal
BIOPOLYMERS
Volume 90, Issue 6, Pages 751-758Publisher
WILEY
DOI: 10.1002/bip.21101
Keywords
protein folding; beta-sheet folding; amide-to-ester backbone mutagenesis; T-jump kinetics; transition state structure
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Funding
- NIH [GM5110]
- The Skaggs Institute of Chemical Biology
- Lita Annenberg Hazen Foundation
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM051105] Funding Source: NIH RePORTER
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Perturbing the structure of the Pin 1 WW domain, a 34-residue protein comprised of three beta-strands and two intervening loops has provided significant insight into the structural and energetic basis of beta-sheet folding. We will review our current perspective on how structure acquisition is influenced by the sequence, which determines local conformational propensities and mediates the hydrophobic effect, hydrogen bonding, and analogous intramolecular interactions. We have utilized both traditional site-directed mutagenesis and backbone mutagenesis approaches to alter the primary structure of this beta-sheet protein. Traditional site-directed mutagenesis experiments are excellent for altering side-chain structure, whereas amide-to-ester backbone mutagenesis experiments modify backbone-backbone hydrogen bonding capacity. The transition state structure associated with the folding of the Pin 1 WW domain features a partially H-bonded, near-native reverse turn secondary structure in loop 1 that has little influence on thermodynamic stability The thermodynamic stability of the Pin 1 WW domain is largely determined by the formation of a small hydrophobic core and by the formation of desolvated backbone-backbone H-bonds enveloped by this hydrophobic core. Loop 1 engineering to the consenus five-residue beta-bulge-turn found in most WW domains or a four-residue beta-turn found in most beta-hairpins accelerates folding substantially relative to the six-residue turn found in the wild type Pin 1 WW domain. Furthermore, the more efficient five-and four-residue reverse turns now contribute to the stability of the three-stranded beta-sheet. These insights have allowed the design of Pin 1 WW domains that fold at rates that approach the theoretical speed limit of folding. (C) 2008 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 90: 751-758, 2008.
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