Article
Chemistry, Medicinal
Monica X. Li, Pascal Mercier, James J. Hartman, Brian D. Sykes
Summary: Tirasemtiv is the first direct fast skeletal troponin activator that selectively and strongly binds to fast skeletal troponin, slowing calcium release rates and sensitizing muscle to calcium. The structure of tirasemtiv bound to a fast skeletal troponin C-troponin I chimera reveals multiple interactions that stabilize the troponin C-troponin I interface and drive the equilibrium toward the active state.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Kirti Magudia, Christopher P. Bridge, Camden P. Bay, Subrina Farah, Ana Babic, Florian J. Fintelmann, Lauren K. Brais, Katherine P. Andriole, Brian M. Wolpin, Michael H. Rosenthal
Summary: This study aimed to determine whether body composition (BC) measurements obtained from routine CT scans using a fully automated deep learning algorithm could predict subsequent cardiovascular events independently from weight, BMI, and additional cardiovascular risk factors. The results showed that VFA derived from automated and normalized abdominal CT analysis predicts the occurrence of myocardial infarction (MI) or stroke in Black and White patients.
AMERICAN JOURNAL OF ROENTGENOLOGY
(2023)
Article
Medicine, Research & Experimental
Eduardo Souza-Silva, Ruy Ascenso, Carlos Rogerio Tonussi, Jose Eduardo da Silva-Santos
Summary: This study aimed to validate an alternative strategy for detecting PORH in rodents and explored the influence of experimental models associated with impaired skeletal muscle function on PORH. The findings showed different profiles of PORH in animals pretreated with various substances or subjected to muscle contraction in stretching, which are commonly used as experimental models for fibromyalgia, type II diabetes mellitus, and soreness induced by unaccustomed eccentric exercise. The study demonstrated that changes in local regulation of blood flow may contribute to the development of skeletal muscle associated symptoms in clinically relevant conditions.
Review
Rehabilitation
David Beckwee, Lotte Cuypers, Nina Lefeber, Emma De Keersmaecker, Ellen Scheys, Wout Van Hees, Stany Perkisas, Sylvie De Raedt, Eric Kerckhofs, Ivan Bautmans, Eva Swinnen
Summary: This systematic review aims to describe the longitudinal changes in skeletal muscles, including muscle strength, muscle mass and muscle volume, during the first 3 months post-stroke. The results showed an increase in muscle strength and decrease in muscle thickness on both the paretic and non-paretic side. Insufficient data were available to draw conclusions about lean muscle mass and muscle cross-sectional area. No studies investigated distinct trajectories of the muscle changes.
JOURNAL OF REHABILITATION MEDICINE
(2022)
Article
Neurosciences
Cameron Hill, Elisabetta Brunello, Luca Fusi, Jesus Garcia Ovejero, Malcolm Irving
Summary: In this study, the activation of myosin filaments in muscle contraction was found to be mainly influenced by filament stress. A switch-like change in myosin motor conformation at the start of force development was also observed, along with the involvement of filament zonal dynamics in activation.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Review
Geriatrics & Gerontology
Qian Zhong, Kun Zheng, Wanmeng Li, Kang An, Yu Liu, Xina Xiao, Shan Hai, Biao Dong, Shuangqing Li, Zhenmei An, Lunzhi Dai
Summary: Skeletal muscle makes up a significant portion of the body mass and plays essential roles in various physiological processes. Muscle health is crucial for overall well-being, and its dysfunction can lead to adverse consequences, particularly in aging individuals. This article investigates the post-translational modifications involved in muscle growth, atrophy, and the development of sarcopenia, providing insights into the underlying mechanisms and potential therapeutic strategies.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Article
Multidisciplinary Sciences
Lei Wang, Xuanyan Guo, Li Tan, Qin Chen
Summary: The study aimed to assess the normal MD elasticity using SWE and found that SWV was significantly higher in males compared to females at both 0 and 90 degrees abduction. MD thickness, age, and BMI did not influence MD elasticity. The reference ranges for normal MD elasticity at different arm abduction positions were established, serving as quantitative baseline measurements for future assessments.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Andrej M. Vilfan, Andreja Sarlah
Summary: In this study, we determined the theoretical efficiency limit of a classical myosin model through numerical calculations. The results showed that the efficiency is influenced by the number of states, stiffness, and rate-limiting kinetic steps, and there is a trade-off between speed and efficiency. Slow myosins with high stiffness and most energy allocated to the working stroke have higher efficiency, while fast myosins with lower stiffness and a larger fraction of energy allocated to the release of ADP perform better.
PLOS COMPUTATIONAL BIOLOGY
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Xiao Liu, Hong-kui Yu, Shu-ya Sheng, Si-min Liang, Hao Lu, Rui-yun Chen, Min Pan, Zhi-bo Wen
Summary: The study found that passive muscle stiffness increases with age, and the differences between age groups only become significant after reaching a certain degree of stretching.
EUROPEAN RADIOLOGY
(2021)
Article
Cell Biology
Mattia Scalabrin, Viktor Engman, Amanda Maccannell, Annabel Critchlow, Lee D. Roberts, Nadira Yuldasheva, T. Scott Bowen
Summary: In hind limb ischemia (HLI), early-stage ischemic muscles show overt remodeling with excessive autophagy signaling, which normalizes over time. Early activation of the sestrin-AMPK signaling axis may regulate autophagy to stimulate rapid and overt muscle atrophy in HLI, which is normalized within weeks and accompanied by recovery of muscle mass. A complex interplay between Sestrins likely regulates autophagy signaling during early-to-late muscle remodeling in HLI.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Review
Biophysics
Wolfgang A. Linke
Summary: This review summarizes the major advances in the discovery of the giant protein titin, including its layout and interactions in muscle sarcomeres, as well as key information about its gene and protein sequence. The mechanical function of titin in skeletal muscle, such as stretch-dependent tension development and elastic diversity, is discussed. The role of titin in active tension development, including length-dependent activation and contraction, is also highlighted.
JOURNAL OF BIOMECHANICS
(2023)
Review
Geriatrics & Gerontology
Madu N. Soares, Moritz Eggelbusch, Elie Naddaf, Karin H. L. Gerrits, Marike van der Schaaf, Bram van den Borst, W. Joost Wiersinga, Michele van Vugt, Peter J. M. Weijs, Andrew J. Murray, Rob C. I. Wust
Summary: Skeletal muscle-related symptoms, including weakness and exercise intolerance, are common in acute Covid-19 and PASC. Severe cases show muscle fibre atrophy, metabolic alterations, and immune cell infiltration. Contributing factors to weakness and fatigue include systemic inflammation, disuse, hypoxemia, and malnutrition. The mechanisms underlying muscle issues in PASC are more complex and may be related to direct viral infiltration or aberrant immune response. Further research is needed to understand the similarities with chronic fatigue syndrome.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2022)
Article
Cell Biology
Alexander Sonne, Lorenzo Peverelli, Aurelio Hernandez-Lain, Cristina Dominguez-Gonzalez, Jesper L. Andersen, Margherita Milone, Alan H. Beggs, Julien Ochala
Summary: In this study, we investigated the effects of MYH2 truncating mutations on the presence and post-translational modifications of myosin heavy chains, as well as the functionality of myosin molecules. We found that these mutations resulted in the presence of type IIa myosin heavy chains with an additional acetylated lysine in patients, accompanied by increased ATP demand, faster actomyosin kinetics, and reduced muscle fiber force.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Medicine, Research & Experimental
Ya-Peng Li, Chun-Long Liu, Zhi-Jie Zhang
Summary: This study found a strong correlation between muscle stiffness measured using the MyotonPRO and muscle activity level, indicating that the MyotonPRO can accurately estimate muscle activity level. Additionally, the MyotonPRO showed good inter-operator reliability for measuring BBM stiffness.
MEDICAL SCIENCE MONITOR
(2022)
Article
Physiology
Leonit Kiriaev, Ben D. Perry, David A. Mahns, Peter J. Shortland, Asma Redwan, John W. Morley, Stewart I. Head
Summary: The study showed that high dose minocycline treatment can reduce maximal isometric force production in fast-twitch EDL and impair protein synthesis in C2C12 cultured cells. These findings are significant for future research on the efficacy of minocycline treatment in neuromuscular disorders or muscle atrophy conditions.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biology
Marko Usaj, Luisa Moretto, Venukumar Vemula, Aseem Salhotra, Alf Mansson
Summary: The benefits of single molecule studies of biomolecules include the need for minimal amounts of material and the potential to reveal phenomena hidden in ensembles. However, recent single molecule studies of fluorescent ATP turnover by myosin are difficult to reconcile with ensemble studies, with key reasons being complexities due to dye photophysics and fluorescent contaminants. After eliminating these complexities, the distributions of ATP binding dwell times on myosin are best described by 2 to 3 exponential processes, providing new insights into ATP turnover mechanisms.
COMMUNICATIONS BIOLOGY
(2021)
Article
Physics, Multidisciplinary
Aseem Salhotra, Jingyuan Zhu, Pradheebha Surendiran, Christoph Robert Meinecke, Roman Lyttleton, Marko Usaj, Frida W. Lindberg, Marlene Norrby, Heiner Linke, Alf Mansson
Summary: Significant progress has been made in utilizing naturally available molecular motors and cytoskeletal filaments for nanotechnological applications, specifically in biosensing and network-based biocomputation. To prolong the function of the biological system, critical factors such as biocompatibility of flow cell components, air exposure, assay solution composition, and nanofabrication methods need to be systematically investigated and optimized. By optimizing these factors, the function of actin and myosin in nanodevices for biocomputation was prolonged from 60 minutes to > 20 hours, demonstrating the importance of using pyranose oxidase and non-fluorescent actin filaments to increase motility run times. Using commercially available microfluidic channel slides, the viability of these approaches in the in vitro motility assay was demonstrated. Additionally, it was shown that myosin motor fragments can be stored at -80 degrees C for over 10 years, indicating the potential for long-term sustainability in network-based biocomputation.
NEW JOURNAL OF PHYSICS
(2021)
Article
Physics, Multidisciplinary
Cordula Reuther, Rachele Catalano, Aseem Salhotra, Venukumar Vemula, Till Korten, Stefan Diez, Alf Mansson
Summary: Over the past 25 years, significant progress has been made in developing nanotechnological applications involving cytoskeletal filaments and molecular motors. Actin-based and microtubule-based systems have different advantages and face distinct challenges when used for tasks such as biosensing and biocomputation. Understanding these differences can facilitate the design of new devices and optimize future developments.
NEW JOURNAL OF PHYSICS
(2021)
Review
Biochemistry & Molecular Biology
Marko Usaj, Luisa Moretto, Alf Mansson
Summary: Hereditary hypertrophic cardiomyopathy (HCM), caused by mutations in sarcomere proteins, is a common and potentially life-threatening condition. The mechanisms underlying the development of the disease are not well understood, but recent research suggests that hypercontractility and inefficient energy usage may play a central role. Further studies are needed to fully understand the pathogenesis of HCM and identify potential therapeutic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Physics, Multidisciplinary
Jingyuan Zhu, Till Korten, Hillel Kugler, Falco van Delft, Alf Mansson, Danny Reuter, Stefan Diez, Heiner Linke
Summary: The research focuses on network-based biocomputation (NBC), a massively parallel approach for solving large NP-complete problem instances. By utilizing biological agents, NBC computers demonstrate higher energy efficiency and greater availability in large numbers compared to electronic computers.
NEW JOURNAL OF PHYSICS
(2021)
Article
Physics, Multidisciplinary
Cordula Reuther, Sonke Steenhusen, Christoph Robert Meinecke, Pradheebha Surendiran, Aseem Salhotra, Frida W. Lindberg, Alf Mansson, Heiner Linke, Stefan Diez
Summary: The study successfully utilized 3D fabrication technology to create junctions with tunnels and overpasses on glass substrates, allowing reliable guidance of microtubules through intersections, thereby opening up new possibilities for implementing 3D functional elements in biomolecular transport systems.
NEW JOURNAL OF PHYSICS
(2021)
Biographical-Item
Physiology
Alf Mansson, Fang Lou, Nancy Curtin, Carlo Reggiani
Article
Multidisciplinary Sciences
Luisa Moretto, Marko Usaj, Oleg Matusovsky, Dilson E. Rassier, Ran Friedman, Alf Mansson
Summary: Release of Pi from the myosin active site is essential for force generation, but the mechanism behind it is not well understood. This study presents evidence for a multistep Pi-release model that reconciles conflicting views and reveals potential functional complexities.
NATURE COMMUNICATIONS
(2022)
Review
Nanoscience & Nanotechnology
Falco C. M. J. M. van Delft, Alf Mansson, Hillel Kugler, Till Korten, Cordula Reuther, Jingyuan Zhu, Roman Lyttleton, Thomas Blaudeck, Christoph Robert Meinecke, Danny Reuter, Stefan Diez, Heiner Linke
Summary: Network-based biocomputation is an alternative parallel computing approach that can potentially solve combinatorial problems with lower energy consumption. Although there have been advancements in proof-of-principle experiments, there are still challenges in scaling up NBC to an industrial level. A roadmap identifying key scientific and technological needs can provide guidance for this expansion.
Article
Biochemistry & Molecular Biology
Alf Mansson, Dilson E. Rassier
Summary: Bottom-up mechanokinetic models predict ensemble function of actin and myosin based on parameter values derived from studies using isolated proteins. This study tests the prediction capability of a model for different drugs and concentrations, and finds that it qualitatively predicts the effects of most drugs but quantitatively fails to account for the effects of one specific drug. By adjusting key model parameters, the modified model not only improves the prediction of the effects of the specific drug, but also maintains good prediction accuracy for normal physiological ensemble function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Physiology
Mikael Derakhti, Domen Bremec, Tim Kambic, Lasse Ten Siethoff, Niklas Psilander
Summary: This study compared the effects of heavy resisted sprint training (RST) versus unresisted sprint training (UST) on sprint performance among adolescent soccer players. The results showed that heavy RST led to superior improvements in short-sprint performance compared with UST, especially for players with a preintervention deficit in force capacity.
INTERNATIONAL JOURNAL OF SPORTS PHYSIOLOGY AND PERFORMANCE
(2022)
Article
Environmental Sciences
Henrik Petre, Alexander Ovendal, Niklas Westblad, Lasse Ten Siethoff, Niklas Psilander
Summary: This study is the first to examine the physical characteristics of elite male bandy players in different positions. Compared to players in the early 1990s, today's bandy players are heavier and have lower relative VO2max, but their absolute VO2max is higher. These findings can serve as a reference for strength and conditioning professionals when designing future training programs.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Chemistry, Physical
Christoph R. Meinecke, Georg Heldt, Thomas Blaudeck, Frida W. Lindberg, Falco C. M. J. M. van Delft, Mohammad Ashikur Rahman, Aseem Salhotra, Alf Mansson, Heiner Linke, Till Korten, Stefan Diez, Danny Reuter, Stefan E. Schulz
Summary: Network-based biocomputation (NBC) relies on nanofabricated channels to guide biological agents accurately. In this study, we present the large-scale fabrication of optimized microfluidic channel networks (NBC networks) using electron-beam lithography. To confirm their functionality, we solve a classical NP-complete problem, the subset-sum problem, on these NBC networks. By optimizing the material stack, nanolithographic fabrication processes, and motor-protein expression and purification, we improve the attachment of functional motor proteins, the smoothness of channel walls and floors, and the motility of filaments. These optimizations increase the reliability of NBC devices and enable the production of larger NBC networks for solving complex combinatorial problems.
Article
Biochemistry & Molecular Biology
Alf Mansson, Marko Usaj, Luisa Moretto, Oleg Matusovsky, Lok Priya Velayuthan, Ran Friedman, Dilson E. Rassier
Summary: The release of Pi from myosin is crucial for energy transduction and the power-stroke. The timing of Pi-release and the power-stroke is poorly understood. Traditional models propose either pre- or post-power-stroke Pi-release, while alternative models suggest a branched kinetic scheme or partial uncoupling. More research is needed to unify these models and enhance our understanding.
Article
Biochemistry & Molecular Biology
Venukumar Vemula, Tamas Huber, Marko Usaj, Beata Bugyi, Alf Mansson
Summary: Research shows that gelsolin and myosin can cooperate under specific conditions to affect cellular signaling and structural rearrangements by altering actin filament severing. Single molecule studies using total internal reflection fluorescence microscopy demonstrate a direct effect of gelsolin binding on the sliding velocity and severing pattern of actin filaments.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)