Article
Multidisciplinary Sciences
Akimitsu Higuchi, Wataru Shihoya, Masae Konno, Tatsuya Ikuta, Hideki Kandori, Keiichi Inoue, Osamu Nureki
Summary: SzRs, a newly identified rhodopsin family in Asgard archaea, exhibit unique kinetic behaviors in inward H+ release compared to other H+ pumps. The crystal structure of SzR AM_5_00977 revealed that key residues and structural features are different from other microbial rhodopsins, suggesting a distinct mechanism for inward H+ release.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Ariel Chazan, Ishita Das, Takayoshi Fujiwara, Shunya Murakoshi, Andrey Rozenberg, Ana Molina-Marquez, Fumiya K. Sano, Tatsuki Tanaka, Patricia Gomez-Villegas, Shirley Larom, Alina Pushkarev, Partha Malakar, Masumi Hasegawa, Yuya Tsukamoto, Tomohiro Ishizuka, Masae Konno, Takashi Nagata, Yosuke Mizuno, Kota Katayama, Rei Abe-Yoshizumi, Sanford Ruhman, Keiichi Inoue, Hideki Kandori, Rosa Leon, Wataru Shihoya, Susumu Yoshizawa, Mordechai Sheves, Osamu Nureki, Oded Beja
Summary: Light-harvesting carotenoids, such as zeaxanthin and lutein, transfer energy to the retinal chromophore of xanthorhodopsins and proteorhodopsins, indicating their potential role in rhodopsin phototrophy in lakes, seas, and oceans.
Article
Chemistry, Multidisciplinary
Juliane Weissbecker, Chokri Boumrifak, Maximilian Breyer, Tristan Wiessalla, Vitaly Shevchenko, Thomas Mager, Chavdar Slavov, Alexey Alekseev, Kirill Kovalev, Valentin Gordeliy, Ernst Bamberg, Josef Wachtveitl
Summary: The new class of microbial rhodopsins, xenorhodopsins (XeRs), extends the versatility of this family by serving as inward H+ pumps, offering an alternative optogenetic tool that can activate electrically excitable cells independently of surrounding physiological conditions. Functional and spectroscopic characterization of NsXeR reveals insights into the photodynamic behavior, pH-dependent kinetics, and potential-dependent vectoriality of photocurrent transients. Mutational studies on the putative intracellular H+ acceptor D220 provide further understanding of the kinetically independent switching mechanism.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Kunisato Kuroi, Takashi Tsukamoto, Naoya Honda, Yuki Sudo, Yuji Furutani
Summary: This study investigated the primary proton transfer reactions of thermophilic rhodopsin and found that it is closely related to the structural change of the protein and optimized at high temperatures, which is not observed in other related proteins.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2023)
Article
Biochemistry & Molecular Biology
Kimleng Chuon, Jin-gon Shim, Se-Hwan Kim, Shin-Gyu Cho, Seanghun Meas, Kun-Wook Kang, Ji-Hyun Kim, Ishita Das, Mordechai Sheves, Kwang-Hwan Jung
Summary: Rhodopsin and carotenoids are molecules utilized by certain bacteria to absorb and utilize light energy. A study has shown that carotenoids act as antennae to harvest light and transfer energy to certain proteins. The interaction between carotenoids and proteins can enhance proton pumping speed and capacity, indicating a potential role in solar energy harvesting.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Kouhei Hayashi, Misao Mizuno, Hideki Kandori, Yasuhisa Mizutani
Summary: Recent discoveries of light-driven inward proton-pumping rhodopsins have provided new insights into the mechanism of unidirectional transport. By studying Schizorhodopsin, it was found that cis-trans thermal reisomerization precedes reprotonation at the Schiff base, shedding light on the mechanism of proton uptake during inward proton pumping.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Xinyue Wang, Shaohua Feng, Weichao Lu, Yingjie Zhao, Sixing Zheng, Wanzhen Zheng, Xiahan Sang, Lirong Zheng, Yu Xie, Zhongjian Li, Bin Yang, Lecheng Lei, Shaobin Wang, Yang Hou
Summary: This study reports a porous Ni-N-C catalyst, ZrO2@Ni-NC, synthesized using a post-synthetic coordination coupling carbonization strategy, which shows high activity and selectivity in CO2RR. The isolated Ni-N-4 species are identified as the real active sites, while the nanostructured ZrO2 accelerates the protonation process, reducing the energy barrier and enhancing catalytic performance.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Chemistry, Physical
Feng Zhu, Wenbin Zhang, Jiao Xun, Bi-Jun Geng, Qing-Man Liang, Yang Yang
Summary: This review focuses on the effects of proton donor/acceptor on several typical electrocatalytical reactions, including species, concentration, pKa, and steric hindrance. Specifically, the proton transfer pathway arising from ligand design strategy of molecular catalysts and modification of electrode-electrolyte interface is presented.
CURRENT OPINION IN ELECTROCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Luca D'Amario, Maria Bruna Stella, Tomas Edvinsson, Maurizio Persico, Johannes Messinger, Holger Dau
Summary: This study describes a transient spectro-electrochemical Raman setup that achieves sub-ms time resolution, allowing for the study of electrochemical processes. The technique was validated through experiments and confirmed to be effective in assigning spectral properties to transient species. This research provides a foundational contribution to the development of next generation electrolizers, fuel cells, and batteries.
Article
Biochemistry & Molecular Biology
Taichi Tsuneishi, Masataka Takahashi, Masaki Tsujimura, Keiichi Kojima, Hiroshi Ishikita, Yasuo Takeuchi, Yuki Sudo
Summary: The study demonstrates that the newly synthesized DMP-retinal can be incorporated into AR3, resulting in novel optical properties and significant differences at the molecular level compared to the natural A1-retinal.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Chemistry, Physical
Michael. J. Lain, Emma Kendrick
Summary: Commercial lithium ion cells were disassembled for electrode evaluation, showing three limiting processes during high rate pulse power tests, and specific charge and discharge parameters were identified for different power levels.
JOURNAL OF POWER SOURCES
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Scott N. Penfold
Summary: The aim of this study was to assess the radiation shielding of proton imaging in a fixed beamline with upright patient positioning system. The study found that pion production had negligible impact on radiation shielding, and the shielding design for proton therapy is adequate for high-energy proton imaging applications.
Article
Materials Science, Ceramics
Jun Ping, Sajid Rauf, Zuhra Tayyab, Changping Yang
Summary: This study investigates the electrochemical performance of polycrystalline spherical LCO in neutral aqueous saturated Li2SO4 solution, demonstrating the stability dependence on discharge cutoff potential. The spherical LCO exhibits good cycling stability and superior rate capability.
CERAMICS INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Alexei A. Stuchebrukhov, Tomoyuki Hayashi
Summary: Using quantum chemistry and electrostatic calculations, the pKa of the reduced quinone in the catalytic cavity of complex I is found to be very high, indicating that a single protonation reaction can drive four protons across the membrane. Based on these calculations, a possible scheme of redox-linked proton pumping by complex I is proposed.
Review
Biochemistry & Molecular Biology
Sergey A. Siletsky, Vitaliy B. Borisov
Summary: Terminal respiratory oxidases are efficient molecular machines that play crucial roles in energy transfer and cell survival. Different types of oxidases have diverse origins and functional significances, yet they all share the ability to avoid the formation of dangerous substances while reducing oxygen to water. Recent advancements in studying their structures and functions are crucial for understanding their roles in cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Jonas Wietek, Silvia Rodriguez-Rozada, Janine Tutas, Federico Tenedini, Christiane Grimm, Thomas G. Oertner, Peter Soba, Peter Hegemann, J. Simon Wiegert
SCIENTIFIC REPORTS
(2017)
Correction
Multidisciplinary Sciences
Jonas Wietek, Silvia Rodriguez-Rozada, Janine Tutas, Federico Tenedini, Christiane Grimm, Thomas G. Oertner, Peter Soba, Peter Hegemann, J. Simon Wiegert
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Christiane Grimm, Arita Silapetere, Arend Vogt, Yinth Andrea Bernal Sierra, Peter Hegemann
SCIENTIFIC REPORTS
(2018)
Article
Biochemistry & Molecular Biology
Roman Fudim, Michal Szczepek, Johannes Vierock, Arend Vogt, Andrea Schmidt, Gunnar Kleinau, Paul Fischer, Franz Bartl, Patrick Scheerer, Peter Hegemann
Article
Biophysics
Arend Vogt, Arita Silapetere, Christiane Grimm, Florian Heiser, Maximiliano Ancina Moeller, Peter Hegemann
BIOPHYSICAL JOURNAL
(2019)
Article
Multidisciplinary Sciences
Jens Kuhne, Johannes Vierock, Stefan Alexander Tennigkeit, Max-Aylmer Dreier, Jonas Wietek, Dennis Petersen, Konstantin Gavriljuk, Samir F. El-Mashtoly, Peter Hegemann, Klaus Gerwert
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Multidisciplinary Sciences
Johannes Oppermann, Paul Fischer, Arita Silapetere, Bernhard Liepe, Silvia Rodriguez-Rozada, Jose Flores-Uribe, Enrico Peter, Anke Keidel, Johannes Vierock, Joel Kaufmann, Matthias Broser, Meike Luck, Franz Bartl, Peter Hildebrandt, J. Simon Wiegert, Oded Beja, Peter Hegemann, Jonas Wietek
NATURE COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Andrey Rozenberg, Johannes Oppermann, Jonas Wietek, Rodrigo Gaston Fernandez Lahore, Ruth-Anne Sandaa, Gunnar Bratbak, Peter Hegemann, Oded Beja
Article
Neurosciences
Mathias Mahn, Inbar Saraf-Sinik, Pritish Patil, Mauro Pulin, Eyal Bitton, Nikolaos Karalis, Felicitas Bruentgens, Shaked Palgi, Asaf Gat, Julien Dine, Jonas Wietek, Ido Davidi, Rivka Levy, Anna Litvin, Fangmin Zhou, Kathrin Sauter, Peter Soba, Dietmar Schmitz, Andreas Luthi, Benjamin R. Rost, J. Simon Wiegert, Ofer Yizhar
Summary: A mosquito homolog of vertebrate encephalopsin was found to effectively suppress synaptic transmission through a specific signaling pathway, triggering lasting synaptic output inhibition that recovers spontaneously in vitro and in vivo. This discovery opens up new avenues for functional exploration of long-range neuronal circuits in vivo.
Review
Neurosciences
Nikolaus Wenger, Arend Vogt, Matej Skrobot, Elisa L. Garulli, Burce Kabaoglu, Christina Salchow-Hoemmen, Thomas Schauer, Daniel Kroneberg, Michael K. Schuhmann, Christoph Harms, Matthias Endres, Ioannis U. Isaias, Philip Tovote, Robert Blum, Chi Wang Ip
Summary: This article summarizes strategies for modeling parkinsonian gait in rodents and discusses the relationship between clinical research findings and motor deficits and network oscillations in animal models. The article suggests that future research should incorporate behavioral tests with higher cognitive demands and emphasizes that basic research should benefit from evolving signal processing strategies from clinical research.
EXPERIMENTAL NEUROLOGY
(2022)
Review
Neurosciences
Benjamin R. Rost, Jonas Wietek, Ofer Yizhar, Dietmar Schmitz
Summary: This review provides a comprehensive overview of the presynaptic applications of optogenetic tools, discussing the challenges, limitations, and future directions. Optogenetic actuators enable precise control of presynaptic functions, such as light-mediated neurotransmitter release, presynaptic inhibition, induction of synaptic plasticity, and manipulation of individual components of the presynaptic machinery.
NATURE NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Andrey Rozenberg, Igor Kaczmarczyk, Donna Matzov, Johannes Vierock, Takashi Nagata, Masahiro Sugiura, Kota Katayama, Yuma Kawasaki, Masae Konno, Yujiro Nagasaka, Mako Aoyama, Ishita Das, Efrat Pahima, Jonathan Church, Suliman Adam, Veniamin A. Borin, Ariel Chazan, Sandra Augustin, Jonas Wietek, Julien Dine, Yoav Peleg, Akira Kawanabe, Yuichiro Fujiwara, Ofer Yizhar, Mordechai Sheves, Igor Schapiro, Yuji Furutani, Hideki Kandori, Keiichi Inoue, Peter Hegemann, Oded Beja, Moran Shalev-Benami
Summary: This study reports a new subfamily of microbial rhodopsins called bestrhodopsins, which are fused with bestrophin channels. Cryo-EM analysis reveals that bestrhodopsins form pentameric megacomplexes and undergo photoconversion. Bestrhodopsins behave as light-modulated anion channels.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2022)
Article
Biology
Silvia Rodriguez-Rozada, Jonas Wietek, Federico Tenedini, Kathrin Sauter, Neena Dhiman, Peter Hegemann, Peter Soba, J. Simon Wiegert
Summary: Aion is a bistable anion-conducting channelrhodopsin that enables long-term silencing of neuronal networks. It can be switched between active and inactive states with precise timing and maintains its activity in the absence of light, making it an ideal tool for optogenetic silencing.
COMMUNICATIONS BIOLOGY
(2022)
Meeting Abstract
Biophysics
Roman Fudim, Arend Vogt, Peter Hegemann
BIOPHYSICAL JOURNAL
(2017)
Meeting Abstract
Biophysics
Christiane Grimm, Arend Vogt, Peter Hegemann
BIOPHYSICAL JOURNAL
(2017)