Article
Multidisciplinary Sciences
Ana C. Laly, Kristina Sliogeryte, Oscar J. Pundel, Rosie Ross, Michael C. Keeling, Deepa Avisetti, Ahmad Waseem, Nuria Gavara, John T. Connelly
Summary: The keratin cytoskeleton plays a critical role in sensing matrix rigidity, with keratinocytes adapting to increased stiffness by forming a rigid interconnected network and boosting cell stiffness. Mutations disrupting keratin stability hinder the normal mechanical response and impair mechanotransduction. Depletion of cytolinker proteins, however, enhances mechanoresponsiveness and up-regulates lamin A/C.
Article
Biochemistry & Molecular Biology
Wei-Chieh Chen, Nien-Chen Hsieh, Mao-Cong Huang, Kai-Chiang Yang, Jiashing Yu, Yang Wei
Summary: In preclinical studies, it has been found that human hair, containing keratin proteins, can be effectively used for hemostasis. However, due to the complex mixture and diverse molecular weights and structures of keratin proteins, their hemostatic capacity varies. To optimize the use of human hair keratin for hemostasis, the effects of different keratin fractions on fibrinogen precipitation were investigated. It was found that a mixture of high molecular weight keratin intermediate filaments (KIFs) and low molecular weight keratin-associated proteins (KAPs) in equal proportions yielded the most extensive precipitation of soluble fibrinogen. However, all hair protein samples showed different catalytic behaviors compared to thrombin, suggesting the potential of utilizing specific hair fractions to develop optimized hair protein-based hemostatic materials.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Genetics & Heredity
David A. D. Parry
Summary: Epidermal appendages of birds and reptiles, including claws, scales, and feathers, have specialized physical properties that facilitate movement, thermal insulation, defence mechanisms, and/or catching prey. The mechanical attributes of these appendages originate from their fibril-matrix texture, with corneous ss-proteins and keratin intermediate filaments providing much of the required tensile properties. Understanding the detailed structures of these components and how they interact with each other is crucial for comprehending the physical properties of these tissues.
Article
Chemistry, Physical
Quoc Dat Pham, Sebastien Gregoire, Bruno Biatry, Guillaume Cassin, Daniel Topgaard, Emma Sparr
Summary: By altering the hydration of the skin, molecules with reasonable solubility in both hydrophobic and hydrophilic environments can be directed to specific regions in the stratum corneum (SC), affecting their distribution and behavior in varying hydration conditions. Hydration serves as a switch to control the location and action of molecules in complex materials like SC, impacting fluid-solid lipid transitions in drying conditions.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Review
Cell Biology
Bowen Yu, Dandan Kong, Chen Cheng, Dongxi Xiang, Longxing Cao, Yingbin Liu, Yongning He
Summary: Keratins play a crucial role in maintaining the mechanical properties of cells and are involved in various cellular activities. However, the structural details and assembly mechanisms of keratins are still poorly understood.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Yi Chen, Chih-Chia Wang, Yu-shuan Ma, Yuan-Yu Chao, Ching-Li Tseng, Yang Wei
Summary: The study investigated the effects of different fractions of keratin on the keratin/PEGDA composite properties, measured porosity and conducted mechanical behavior tests to determine material strengths. The gel properties highly correlated with the content of KAPs/KIFs, showing that modulating this ratio leads to a keratin/PEGDA composite with more controllable gel properties.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Liling Chen, Run Meng, Rui Qing, Wenfeng Li, Ziwei Wang, Yao Hou, Jia Deng, Wei Pu, Zibin Gao, Bochu Wang, Shilei Hao
Summary: We have successfully developed robust keratin hydrogels based on mechanism study through recombinant proteins, which outperform combinations of native proteins in mechanical properties and in biomedical applications.
Article
Biology
Miriam S. Weber, Matthias Eibauer, Suganya Sivagurunathan, Thomas M. Magin, Robert D. Goldman, Ohad Medalia
Summary: This study analyzed the structure of keratin 5/keratin 14 filaments within ghost mouse keratinocytes using cryo-electron microscopy and revealed the complex and remarkable heterogenic architecture of keratin filaments, suggesting that they are highly flexible, dynamic cytoskeletal structures. Cross-section views of filaments showed that keratins form hollow cylinders consisting of multiple protofilaments, with an electron dense core located in the center of the filament.
Article
Multidisciplinary Sciences
Ondrej Kucera, Jeremie Gaillard, Christophe Guerin, Manuel Thery, Laurent Blanchoin
Summary: By combining microtubules and actin filaments and utilizing a feedback loop, we have created an artificial active cytoskeletal composite that exhibits both structural stability and plasticity. The system is sensitive to external stimuli and shows potential for self-regulation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biology
Jaakko Lehtimaki, Eeva Kaisa Rajakyla, Sari Tojkander, Pekka Lappalainen
Summary: The study reveals that stress fibers can form without pre-existing actomyosin bundles, giving rise to structures known as cortical stress fibers, which are embedded in the cell cortex and assemble preferentially underneath the nucleus. The assembly process is orchestrated by non-muscle myosin II pulses, leading to the reinforcement of nascent focal adhesions and subsequent stabilization of the cortical stress fibers. These findings demonstrate a new mechanism for de novo generation of stress fibers from the actin cortex, highlighting the role of stochastic myosin pulses in the assembly of functional actomyosin bundles.
Review
Oncology
Xin-Yuan Wei, Jie Zhao, Hao-Bin Tong, Shang-Jie Cheng, Na He, Fei-Xue Song
Summary: KRT80 is a human epithelial intermediate filament type II gene that is widely expressed in almost all epithelial cells. It plays an important role in promoting proliferation, migration, and invasiveness of cancer cells, and has a potential as a therapeutic target for neoplasms.
Review
Biochemistry & Molecular Biology
Anton S. Shakhov, Irina B. Alieva
Summary: The endothelium plays a key role in the transcytosis of lipoproteins. Vimentin, as a main protein of endothelial intermediate filaments, is involved in cell polarization, migration, and control over endothelial cell functions. Vimentin also serves as an indicator of cardiovascular system state and participates in the development of atherosclerosis.
Article
Cell Biology
Sonia Ratajczyk, Corinne Drexler, Reinhard Windoffer, Rudolf E. Leube, Peter Fuchs
Summary: Epiplakin (EPPK1) dynamically relocalizes and associates with keratin filaments to stabilize the keratin network under stress conditions, which involves an increase in cytoplasmic Ca2+ levels.
Article
Biochemistry & Molecular Biology
Afrooz Dabbaghizadeh, Alexandre Pare, Zacharie Cheng-Boivin, Robin Dagher, Sandra Minotti, Marie-Josee Dicaire, Bernard Brais, Jason C. Young, Heather D. Durham, Benoit J. Gentil
Summary: Autosomal Recessive Spastic Ataxia of the Charlevoix Saguenay (ARSACS) is a genetic disease caused by mutation in the SACS gene, resulting in loss of function of the protein sacsin. The SacsJ domain of sacsin plays a key role in regulating the neurofilament (NF) and vimentin intermediate filament (IF) networks in cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Physics, Multidisciplinary
Saad Ansari, Wen Yan, Adam Ray Lamson, Michael J. Shelley, Matthew A. Glaser, Meredith D. Betterton
Summary: Living systems exhibit self-organization, and the self-assembly of cytoskeletal networks is spatially constrained by membranes and organelles. Active condensation of cytoskeletal filaments occurs in the presence of crosslinking motor proteins, with confinement altering the morphology of the condensates.
FRONTIERS IN PHYSICS
(2022)
Article
Chemistry, Medicinal
Kang-Hoon Kim, Won-Seok Chung, Yoomi Kim, Ki-Suk Kim, In-Seung Lee, Ji Young Park, Hyeon-Soo Jeong, Yun-Cheol Na, Chang-Hun Lee, Hyeung-Jin Jang
PHYTOTHERAPY RESEARCH
(2015)
Article
Biophysics
Jin Seob Kim, Chang-Hun Lee, Baogen Y. Su, Pierre A. Coulombe
BIOPHYSICAL JOURNAL
(2012)
Review
Dermatology
Pierre A. Coulombe, Chang-Hun Lee
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2012)
Article
Neurosciences
SeungYeong Im, Jinju Jeong, Gwonhyu Jin, Jiwoo Yeom, Janghwan Jekal, Sang-im Lee, Jung Ah Cho, Sukkyoo Lee, Youngmi Lee, Dae-Hwan Kim, Mijeong Bae, Jinhwa Heo, Cheil Moon, Chang-Hun Lee
NEUROSCIENCE LETTERS
(2019)
Correction
Neurosciences
SeungYeong Im, Jinju Jeong, Gwonhyu Jin, Jiwoo Yeom, Janghwan Jekal, Sang-Im Lee, Jung Ah Cho, Sukkyoo Lee, Youngmi Lee, Dae-Hwan Kim, Mijeong Bae, Jinhwa Heo, Cheil Moon, Chang-Hun Lee
NEUROSCIENCE LETTERS
(2019)
Article
Multidisciplinary Sciences
Seung Yeong Im, Jinju Jeong, Gwonhyu Jin, Jiwoo Yeom, Janghwan Jekal, Sang-im Lee, Jung Ah Cho, Sukkyoo Lee, Youngmi Lee, Dae-Hwan Kim, Mijeong Bae, Jinhwa Heo, Cheil Moon, Chang-Hun Lee
SCIENTIFIC REPORTS
(2019)
Correction
Multidisciplinary Sciences
SeungYeong Im, Jinju Jeong, Gwonhyu Jin, Jiwoo Yeom, Janghwan Jekal, Sang-im Lee, Jung Ah Cho, Sukkyoo Lee, Youngmi Lee, Dae-Hwan Kim, Mijeong Bae, Jinhwa Heo, Cheil Moon, Chang-Hun Lee
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Chang-Hun Lee, Min-Sung Kim, Shuang Li, Daniel J. Leahy, Pierre A. Coulombe
Article
Chemistry, Physical
Je-Un Jeong, Dae-Yun Ji, Kwon-Yeong Lee, Woonbong Hwang, Chang-Hun Lee, Sung-Jae Kim, Jeong-Won Lee
Summary: The study highlights the importance of superhydrophobic surfaces being able to resist attached condensation in supersaturated conditions to improve heat transfer efficiency, which can be achieved by controlling the gap size and complexity of micro/nanoscale surface structures to maintain water repellency.
Article
Biochemistry & Molecular Biology
Bongki Cho, Seung-Jun Yoo, So Yeon Kim, Chang-Hun Lee, Yun-Il Lee, Seong-Ryong Lee, Cheil Moon
Summary: In this study, divergently modified peptide analogs derived from EPO showed potent neuroprotective effects against oxidative stress without inducing cell proliferation. One of the peptides, ML1-h3, exhibited unique binding potency to EPOR and inhibited neuronal death and brain injury under hypoxic stress.
Review
Medicine, Research & Experimental
Arokia Vijaya Anand Mariadoss, Allur Subramaniyan Sivakumar, Chang-Hun Lee, Sung Jae Kim
Summary: This review summarizes the scientific information and therapeutic management of diabetes mellitus (DM) and diabetic foot ulcers (DFU) using nanomedicine. Standard drugs and hypoglycemic medications are commonly used, but they have limited effectiveness and can cause side effects. Research is ongoing to explore nanoparticle-based medications that can avoid these side effects.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Chemistry, Physical
Je-Un Jeong, Yoon-Gi Heo, Jung-Ah Cho, Yoojin Roh, Sung Jae Kim, Chang-Hun Lee, Jeong-Won Lee
Summary: In recent decades, superhydrophobic surfaces and slippery liquid-infused porous surfaces (SLIPS) have been researched for industrialization due to their superior antibiofilm performance. This study focuses on surface modification methods for TiAl6V4, a representative metal used in the human body, and evaluates the antibiofilm performance of each fabricated surface. The results show that TiAl6V4 with micro-nanostructured or nanostructured superhydrophobic surface effectively suppresses biofilm generation when exposed to bacteria.
JOURNAL OF ALLOYS AND COMPOUNDS
(2022)
Article
Multidisciplinary Sciences
Jung-Ah Cho, Yoo Jin Roh, Hye Rim Son, Hojung Choi, Jeong-Won Lee, Sung Jae Kim, Chang-Hun Lee
Summary: Biofilm formation is a major cause of complications after surgery. This study investigated the biofilm-forming abilities of periprosthetic infection-associated pathogens on different surfaces and examined the effects of the external stressor EGCG. The findings help in understanding the biofilm-forming abilities of these pathogens and provide insights for further research.
SCIENTIFIC REPORTS
(2022)
Article
Medicine, Research & Experimental
SeungYeong Im, Gwonhyu Jin, Jinju Jeong, Jiwoo Yeom, Janghwan Jekal, Sang-im Lee, Jung Ah Cho, Sukkyoo Lee, Youngmi Lee, Dae-Hwan Kim, Mijeong Bae, Jinhwa Heo, Cheil Moon, Chang-Hun Lee
EXPERIMENTAL NEUROBIOLOGY
(2018)
Review
Endocrinology & Metabolism
Chang-Hun Lee
ENDOCRINOLOGY AND METABOLISM
(2017)