Article
Multidisciplinary Sciences
Heidi Ulrichs, Ignas Gaska, Shashank Shekhar
Summary: Cells regulate actin assembly by controlling reactions at actin filament barbed ends. Three proteins, formin, CP, and twinfilin, have distinct roles at these barbed ends. Using microfluidics-assisted TIRF microscopy, it was found that all three proteins can bind filament barbed ends simultaneously. Single-molecule experiments showed that twinfilin cannot bind barbed ends occupied by formin in the presence of CP. This study establishes a paradigm where polymerases, depolymerases, and cappers together tune actin assembly.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Marina Marchenko, Victoria Nefedova, Natalia Artemova, Sergey Kleymenov, Dmitrii Levitsky, Alexander Matyushenko
Summary: This study investigates the structural and functional differences of five cytoplasmic Tpm isoforms, revealing that sequence variations in alternatively spliced regions of these isoforms can significantly impact their interaction with actin filaments, thus playing a crucial role in cytoskeleton organization and dynamics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Plant Sciences
Min Diao, Shanjin Huang
Summary: Cell-to-cell communication in plants is mediated by plasmodesmata with regulated permeability. Actin cytoskeleton is involved in regulating the permeability, with recent studies on PD-localized formin proteins shedding light on their role and mechanism in intercellular trafficking. This mini-review article describes the progress in understanding these mechanisms.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Sarmini Nageswaran, Juliane Haipeter, Jonathan F. E. Bodenschatz, Ruth Meyer, Sarah Koester, Claudia Steinem
Summary: Within a cell, intermediate filaments play an important role in maintaining mechanical stability. However, little attention has been given to their interaction with the plasma membrane. In this study, we developed an in vitro model to investigate how intermediate filaments react to membrane stretching. We found that the filaments undergo reorganization and elongation in response to stretching, especially in a dense network.
Article
Cell Biology
Laura A. Sherer, Naomi Courtemanche
Summary: The assembly of actin-based structures relies on both polymerization and crosslinking, which play interdependent roles in shaping the architectures of actin bundles. In this study, we investigated the impact of filament elongation on the formation of actin bundles by the crosslinking protein fascin. Our findings demonstrate that filament length affects the rate of bundle assembly, with longer filaments promoting bundle coalescence into interconnected networks. However, the connections formed between elongating bundles are short-lived and often followed by filament breakage. These results highlight the importance of maintaining precise control over filament length during the assembly of specialized actin structures.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Sabrina Pospich, Florian Kuellmer, Veselin Nasufovic, Johanna Funk, Alexander Belyy, Peter Bieling, Hans-Dieter Arndt, Stefan Raunser
Summary: This study presents the structures of a cell-permeable optojasp bound to actin filaments and highlights the effects of photoswitching a functionalized azobenzene for the first time. Analyzing the binding site and conformational changes within F-actin dependent on the optojasp isomeric state refines the determinants for designing functional F-actin photoswitches.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Multidisciplinary Sciences
Julian Knerr, Ralf Werner, Carsten Schwan, Hong Wang, Peter Gebhardt, Helga Groetsch, Almuth Caliebe, Malte Spielmann, Paul-Martin Holterhus, Robert Grosse, Nadine C. Hornig
Summary: Steroid hormone receptors are crucial for mammalian physiology and are involved in various conditions. Functional mutations in DAAM2 gene were found in patients with androgen insensitivity syndrome. DAAM2 is enriched in the nucleus and forms actin-dependent transcriptional droplets with AR in response to dihydrotestosterone, promoting gene expression and cellular functions. Our study reveals the signal-regulated nuclear actin assembly at a steroid hormone receptor, which is necessary for transcription.
Article
Cardiac & Cardiovascular Systems
Larissa M. Dorsch, Diederik W. D. Kuster, Jan D. H. Jongbloed, Ludolf G. Boven, Karin Y. van Spaendonck-Zwarts, Albert J. H. Suurmeijer, Aryan Vink, Gideon J. du Marchie Sarvaas, Maarten P. van den Berg, Jolanda van der Velden, Bianca J. J. M. Brundel, Paul A. Van der Zwaag
Summary: Variants within the alpha-tropomyosin gene (TPM1) can lead to different effects on cardiomyocyte physiology, resulting in various manifestations of cardiomyopathies. Overexpression of different TPM1 variants can progressively deteriorate CaT and result in loss of sarcomeric structures.
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marina A. Marchenko, Victoria V. Nefedova, Daria S. Yampolskaya, Vera A. Borzova, Sergey Y. Kleymenov, Salavat R. Nabiev, Larisa V. Nikitina, Alexander M. Matyushenko, Dmitrii I. Levitsky
Summary: This study investigated the properties of five low molecular weight Tpm isoforms and found significant differences in their sequences variations in alternatively spliced regions, which may be important for further research on the organization and dynamics of the cytoskeleton.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Cell Biology
Qing Tang, Luther W. Pollard, Kaitlin E. Homa, David R. Kovar, Kathleen M. Trybus
Summary: It was proposed that S. pombe tropomyosin Cdc8 (Tpm) segregates into two populations based on the presence or absence of amino-terminal acetylation, which determines its binding to formin-mediated F-actin networks. However, there was no supporting biochemistry. In vitro experiments using recombinant S. pombe proteins showed that acetyl-Tpm tightly binds to actin, while unacetylated Tpm binds weakly. Contrary to the differential recruitment model, Tpm did not show preferential binding to filaments assembled by the FH1-FH2 domains of two S. pombe formins.
Article
Chemistry, Multidisciplinary
Kodai Fukumoto, Yuya Miyazono, Takuya Ueda, Yoshie Harada, Hisashi Tadakuma
Summary: Cellular transport systems are complex and efficient, and there is a need to design artificial transport systems using nanotechnology. However, the design principles have been difficult to establish due to the lack of understanding on how motor layout affects motile activity. In this study, a DNA origami platform was used to evaluate the effect of motor protein layout on transporter motility. The densely packed layout of kinesin motor protein was found to decrease the run length of the transporter, highlighting the importance of considering steric hindrance in transport system design.
NANOSCALE ADVANCES
(2023)
Article
Biochemistry & Molecular Biology
Chanchanok Chaichim, Tamara Tomanic, Holly Stefen, Esmeralda Paric, Lucy Gamaroff, Alexandra K. Suchowerska, Peter W. Gunning, Yazi D. Ke, Thomas Fath, John Power
Summary: The study found that, despite being enriched in dendritic spines, overexpression of Tpm3.1 does not significantly alter the morphology or function of dendritic spines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Anthony P. Baldo, Jil C. Tardiff, Steven D. Schwartz
Summary: This study explores how tropomyosin movement on actin filaments regulates the cross-bridge cycle of cardiac muscle, with experimental information lacking on how myosin binds to the thin filament and influences tropomyosin's transition. Using metadynamics, the researchers found differences in required work due to cardiac troponin T's influence, leading to preferential myosin binding sites and determining the mechanism of further myosin head recruitment.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Arit Ghosh, Megan Coffin, Richard West, Velia M. Fowler
Summary: Tmod3 plays a crucial role in erythroid differentiation by nucleating F-actin assembly and promoting the incorporation of Tmod1 and Tpms into the membrane skeleton, which is essential for morphological maturation and cell survival.
Review
Biochemistry & Molecular Biology
Dmitri Tolkatchev, Carol C. Gregorio, Alla S. Kostyukova
Summary: Leiomodin is an important emerging regulator of thin filaments, and structural and cellular studies have helped elucidate its function mechanisms, with many domains still remaining unstudied. Future investigations may provide evidence that leiomodin is a multifunctional protein.
Article
Thermodynamics
Veronika Takacs-Kollar, Miklos Nyitrai, Denes Lorinczy, Gabor Hild
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2017)
Article
Thermodynamics
Veronika Takacs-Kollar, Miklos Nyitrai, Denes Lorinczy, Gabor Hild
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2018)
Article
Neurosciences
Tams Kovacs-Oller, Gabor Debertin, Marton Balogh, Alma Ganczer, Jozsef Orban, Miklos Nyitrai, Lajos Balogh, Orsolya Kantor, Bela Volgyi
FRONTIERS IN CELLULAR NEUROSCIENCE
(2017)
Article
Pharmacology & Pharmacy
Aliz Szabo, Katalin Sumegi, Katalin Fekete, Eniko Hocsak, Balazs Debreceni, Gyorgy Setalo, Krisztina Kovacs, Laszlo Deres, Andras Kengyel, Dominika Kovacs, Jozsef Mandl, Miklos Nyitrai, Mark A. Febbraio, Ferenc Gallyas, Balazs Sumegi
BIOCHEMICAL PHARMACOLOGY
(2018)
Article
Biophysics
Nikolett Kis-Bicskei, Balint Becsi, Ferenc Erdodi, Robert C. Robinson, Beata Bugyi, Tamas Huber, Miklos Nyitrai, Gabor Csaba Talian
BIOPHYSICAL JOURNAL
(2018)
Article
Spectroscopy
Yin Li, Andras Lukacs, Sandor Bordacs, Janos Moczar, Miklos Nyitrai, Janos Hebling
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2018)
Article
Multidisciplinary Sciences
David Szatmari, Bo Xue, Balakrishnan Kannan, Leslie D. Burtnick, Beata Bugyi, Miklos Nyitrai, Robert C. Robinson
Article
Chemistry, Analytical
Henriett Halasz, Ali Reza Ghadaksaz, Tamas Madarasz, Krisztina Huber, Gabor Harami, Eszter Angela Toth, Aniko Osteikoetxea-Molnar, Mihaly Kovacs, Zsolt Balogi, Miklos Nyitrai, Janos Matko, Edina Szabo-Meleg
METHODS AND APPLICATIONS IN FLUORESCENCE
(2018)
Article
Thermodynamics
P. Maroti, P. Varga, A. Ferencz, Z. Ujfalusi, M. Nyitrai, D. Lorinczy
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2019)
Article
Materials Science, Multidisciplinary
Peter Maroti, Peter Varga, Hajnalka Abraham, Gyorgy Falk, Tamas Zsebe, Zoltan Meiszterics, Sandor Mano, Zoltan Csernatony, Szilard Rendeki, Miklos Nyitrai
MATERIALS RESEARCH EXPRESS
(2019)
Article
Materials Science, Multidisciplinary
P. Varga, D. Lorinczy, L. Toth, A. Pentek, M. Nyitrai, P. Maroti
MATERIALS RESEARCH EXPRESS
(2019)
Article
Thermodynamics
P. Maroti, B. Kocsis, A. Ferencz, M. Nyitrai, D. Lorinczy
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2020)
Article
Multidisciplinary Sciences
Adam Jonatan Tengolics, Gergely Szarka, Alma Ganczer, Edina Szabo-Meleg, Miklos Nyitrai, Tamas Kovacs-Oller, Bela Volgyi
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Kristof Karadi, Sofia M. Kapetanaki, Katalin Raics, Ildiko Pecsi, Robert Kapronczai, Zsuzsanna Fekete, James N. Iuliano, Jinnette Tolentino Collado, Agnieszka A. Gil, Jozsef Orban, Miklos Nyitrai, Greg M. Greetham, Marten H. Vos, Peter J. Tonge, Stephen R. Meech, Andras Lukacs
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Eszter A. Toth, Adam Oszvald, Maria Peter, Gabor Balogh, Aniko Osteikoetxea-Molnar, Tamas Bozo, Edina Szabo-Meleg, Miklos Nyitrai, Imre Derenyi, Miklos Kellermayer, Toshiyuki Yamaji, Kentaro Hanada, Laszlo Vigh, Janos Matko
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2017)