Journal
BIOPHYSICAL CHEMISTRY
Volume 162, Issue -, Pages 1-5Publisher
ELSEVIER
DOI: 10.1016/j.bpc.2011.11.002
Keywords
Poly-L-glutamate; PPII; 2.5(1)-Helix; Salt exclusion; UV resonance Raman
Funding
- NIH [1 RO1EB009089]
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CD and UV resonance Raman measurements surprisingly find that the charge screening of even 2 M concentrations of NaCl and KCl does not alter the unfolded PPII and 2.5(1)-helix conformations of poly-L-glutamate. These salts appear to be excluded from the region between the side chain charges and the peptide backbone. Furthermore, no direct ion pairing occurs between these salts and the side chain carboxylates. (C) 2011 Elsevier B.V. All rights reserved.
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