Article
Spectroscopy
Huimin Shangguan, Qianwei Liu, Yuanjuan Wang, Zixuan Teng, Rumeng Tian, Tong Wu, Lei Yang, Ling Jiang, Xingjiang Liu, Liuhe Wei
Summary: In this study, a chromenoquinoline-based ratiometric fluorescent probe was developed for the detection and quantification of hypochlorous acid (HClO). The probe exhibited a large Stokes shift, high synthetic yield, simple synthesis method, short response time, low detection limit, and low toxicity. The probe was successfully validated in live cells and zebrafish.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Peraya Hiranmartsuwan, Sirilak Wangngae, Jukkrit Nootem, Anyanee Kamkaew, Rathawat Daengngern, Worawat Wattanathana, Kantapat Chansaenpak
Summary: In this study, two pyridylhydrazone-tethered BODIPY compounds were synthesized and characterized. These compounds have the ability to selectively detect hypochlorous acid (HOCl) through a fluorescence turn-on mechanism. In cell imaging experiments, they showed excellent biocompatibility and visualization of endogenous HOCl in living cells.
Article
Chemistry, Analytical
Feng Ding, Han Wen, Ruijiang Zhuo, Jiawei Li, Hui Zheng, Yuru Deng, Jianliang Shen, Xiaojun He
Summary: This study presents a novel chemosensor CMTH based on coumarin for highly sensitive and selective detection of HClO in various organisms, with ultrafast response time and good water solubility being its key features.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Applied
Guangshuai Zhou, Shaobo Hou, Ning Zhao, Nathaniel Finney, Yali Wang
Summary: A highly sensitive ratiometric fluorescent probe, NAP-RS, was designed for measuring fluctuations of lysosomal HOCl. The probe exhibited a clear fluorescence response to HOCl at biologically-relevant concentrations, with rapid response time and ultrasensitivity. Additionally, it could be used for visualizing exogenous and endogenous lysosomal HOCl.
Article
Spectroscopy
Yaqian Li, Yi Zhou, Xiu Liu, Jieni Lei, Xin Qin, Guangyi Li, Zi Yang
Summary: In this paper, a near-infrared ratiometric fluorescent probe (YQ-1) was developed to detect hypochlorous acid (HClO) for comprehending its biological functions within cells. YQ-1 exhibited a fluorescence shift from red to green with a large blue shift (165 nm) in the presence of HClO, and the solution's color changed from pink to yellow. YQ-1 quickly detected HClO (within 40 s) with a low detection limit (4.47 x 10-7 mol/L) and was not affected by other interferences.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Multidisciplinary
Wei Tuo, Jean-Philippe Renault, Aashini Rajpal, Serge Pin, Thierry Le Gall, Frederic Taran
Summary: This study presents the use of benzothiazolines as radiosensitive compounds that can produce gamma-irradiation and fluorescent benzothiazoles, as well as release amines or carboxylic acids upon treatment with Cs-137 in water. The proof of concept study demonstrated that benzothiazolines can be utilized as cleavable linkers that are triggered by irradiation.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Analytical
Hao Zhang, Qincong Feng, Ji-Ting Hou, Zhipeng Li, Jianliang Shen
Summary: Despite being considered as a harmful ROS in cells, hypochlorous acid (HClO/ClO-) plays a crucial role in various physiological and pathological processes. This study introduces a novel carboxy Bodipy-based probe, Bp-S, which exhibits high selectivity, sensitivity, and resistance to photobleaching for the detection of ClO-. The fluorescence imaging of Bp-S successfully detected changes in exogenous/endogenous ClO- in Hela cells and RAW264.7 cells, suggesting its potential in disease diagnosis and research.
Article
Chemistry, Analytical
Miao Chen, Xiaoyu Chen, Yu Wang, Xuhong Fan, Tongsheng Chen, Zhenhao Liang
Summary: In this study, an ESIPT fluorescent probe, MEBTA-Cl, was developed for highly sensitive and low detection limit HClO detection. The probe showed rapid response, high sensitivity, and was successfully used for detecting HClO in living cells and zebrafish.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Applied
Yadan Huo, Yuan Ji, Dingguo Chen, Shouzhi Pu
Summary: The study presents the design of a mitochondrial-targeted probe HDCl for fast and sensitive detection of HClO in living cells, which can be successfully applied for mitochondrial imaging.
Article
Chemistry, Applied
Xiaoli Qian, Hui Yu, Wenchao Zhu, Xufeng Yao, Wangwang Liu, Shikui Yang, Fangyuan Zhou, Yi Liu
Summary: The new fluorescence HOCl probe NFL-S combines high sensitivity and selectivity, successfully detecting endogenous HOCl and functioning in oxidative stress imaging.
Article
Materials Science, Biomaterials
Jia Ke, Peixu Zhao, Jianfeng Li, Qiang Fu
Summary: In this study, a two-photon fluorogenic probe named Q-HOCl was used for the specific and sensitive detection of hypochlorous acid (HOCl) in Alzheimer's disease (AD) brains. The probe showed excellent selectivity and rapid response to HOCl, with a detection limit of 12.5 nM. The elevated level of HOCl in AD brains compared to normal brains was observed, and treatment with an MPO inhibitor effectively improved the cognitive ability of AD model mice.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Spectroscopy
Biao Gu, Mengqin Liu, Jiumei Long, Xinrong Ye, Zhifeng Xu, Youming Shen
Summary: In this contribution, a novel AIE-based fluorescent chemosensor has been developed for the rapid and accurate detection of intracellular hypochlorous acid (HClO). The probe demonstrates high sensitivity and selectivity towards HClO and is capable of imaging exogenous and endogenous HClO in living cells through dual channels. This research has potential applications in the biological and environmental fields.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Spectroscopy
Ruirui Zhang, Lili Lian, Bingya Wang, Lei Zhu, Yueping Ren, Jianliang Shen, Xiao-Qi Yu, Ji-Ting Hou
Summary: This study presents a fluorescent probe BBPy for the detection of hypochlorous acid (HOCl) based on the oxidation of phenothiazine. It demonstrates that HOCl can be a promising biomarker for diabetic cataract (DC) and fluorescence imaging technique can be considered as a candidate tool for DC diagnosis.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Gauri S. Malankar, Arunima Sakunthala, Ambuja Navalkar, Samir K. Maji, Saravanan Raju, Sudesh T. Manjare
Summary: Phenylselenide substituted BOPHY probes show high sensitivity and selectivity towards hypochlorous acid in water, with rapid and reversible reaction, suitable for bioimaging studies.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Multidisciplinary
Haixian Ren, Fangjun Huo, Caixia Yin
Summary: Hypochlorous acid, with low physiological concentration, is crucial in killing pathogens and has anti-inflammatory properties in the human immune system. Sensitively detecting tiny concentration changes of HOCl is necessary for understanding inflammation-associated diseases and cancer. The probe JBD effectively detects endogenous hypochlorous acid and provides insights into its role in oxidative stress and immune responses.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Engineering, Biomedical
Qian Qin, Zhaojun Yin, Xuanjun Wu, Karen M. Haas, Xuefei Huang
Article
Chemistry, Multidisciplinary
Bijuan Lin, Xuanjun Wu, Hu Zhao, Yunpeng Tian, Jiahuai Han, Jian Liu, Shoufa Han
Article
Chemistry, Multidisciplinary
Xuanjun Wu, Zhaojun Yin, Craig McKay, Christian Pett, Jin Yu, Manuel Schorlemer, Trevor Gohl, Suttipun Sungsuwan, Sherif Ramadan, Claire Baniel, Anthony Allmon, Rupali Das, Ulrika Westerlind, M. G. Finn, Xuefei Huang
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2018)
Article
Biochemistry & Molecular Biology
Xuanjun Wu, Craig McKay, Christian Pett, Jin Yu, Manuel Schorlemer, Sherif Ramadan, Shuyao Lang, Sandra Behren, Ulrika Westerlind, M. G. Finn, Xuefei Huang
ACS CHEMICAL BIOLOGY
(2019)
Article
Chemistry, Multidisciplinary
Xianwu Wang, Shuyao Lang, Yunpeng Tian, Jianghong Zhang, Xu Yan, Zhihong Fang, Jian Weng, Na Lu, Xuanjun Wu, Tianlu Li, Hongzhi Cao, Zhu Li, Xuefei Huang
ACS CENTRAL SCIENCE
(2020)
Article
Chemistry, Multidisciplinary
Yanan Gao, Qingyu Zhao, Huiling Dong, Min Xiao, Xuefei Huang, Xuanjun Wu
Summary: Cytotoxic T-lymphocytes (CTLs) play a central role in protective immunity against malignancies and infectious diseases. This study developed partially oxidized acetalated dextran nanoparticles (Ox-AcDEX NPs) as a general vaccine delivery platform. By conjugating CTL peptide epitopes and immune-enhancing adjuvants, effective vaccines against cancer and viruses were successfully created, demonstrating the potential for immune protection.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Xuanjun Wu, Jinfeng Ye, Andrew T. DeLaitsch, Zahra Rashidijahanabad, Shuyao Lang, Tayeb Kakeshpour, Yuetao Zhao, Sherif Ramadan, Paulo Vilar Saavedra, Vilma Yuzbasiyan-Gurkan, Herbert Kavunja, Hongzhi Cao, Jeffrey C. Gildersleeve, Xuefei Huang
Summary: The new GD2 derivative 9NHAc-GD2 conjugated with a powerful carrier bacteriophage Q beta was able to induce strong and selective IgG antibodies in mice, while showing immunogenicity in canines with minimal adverse effects. This paves the way for potential clinical translation to humans in the future.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Engineering, Biomedical
Yanan Gao, Qingyu Zhao, Min Xiao, Xuefei Huang, Xuanjun Wu
Summary: The study investigates synergistic therapy combining immunotherapy with photothermal effect for targeting tumors. Administration of a multifunctional vaccine enhances antitumor CTL immunotherapy.
Article
Materials Science, Biomaterials
Huiling Dong, Yanan Gao, Xuefei Huang, Xuanjun Wu
Summary: This study synthesized two ICG conjugate theranostics and found that Sia-C9-ICG has excellent tumor targeting and prolonged circulation properties, making it a promising treatment for cancer.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Chemistry, Multidisciplinary
Yanan Gao, Wei Wang, Yunru Yang, Qingyu Zhao, Chendong Yang, Xiaoying Jia, Yang Liu, Minmin Zhou, Weihong Zeng, Xuefei Huang, Sandra Chiu, Tengchuan Jin, Xuanjun Wu
Summary: Researchers have developed functionalized nanoparticles as a universal vaccine design platform for major diseases such as cancer and COVID-19. The nanoparticles induce potent immune responses and provide effective protection against tumors and the SARS-CoV-2 virus.
Article
Materials Science, Biomaterials
Huiling Dong, Xuefei Huang, Xuanjun Wu
Summary: 9-SH-Sia is coupled to CPT to form a novel CPT prodrug (CPT-ss-Sia) that self-assembles into nanostructures in an aqueous solution. CPT-ss-Sia exhibits enhanced water solubility, GSH-triggered CPT release, and increased E-lactone ring stability, with comparable cancer cell-killing ability to CPT. Intravenous administration of CPT-ss-Sia inhibits tumor growth and reduces lesions without adverse effects on body weight in mice. This is the first report of the 9-SH-Sia conjugate-based prodrug, with broad clinical application prospects.
BIOMATERIALS SCIENCE
(2023)
Article
Materials Science, Biomaterials
Jiale Wang, Chendong Yang, Yanan Gao, Xuanjun Wu
Summary: Developing safe and high-quality theranostic agents for cancer treatment is crucial. In this study, the clinical indocyanine green (ICG) was coupled with biocompatible poly(styrene-alt-maleic anhydride) (PSMAn) to obtain PSMAn-ICG polymer. The self-assembly of its hydrolyzed product in water resulted in ICG-conjugated poly(styrene-alt-maleic acid) nanoparticles (PSMA-ICG NPs). These NPs demonstrated good solubility and stability in aqueous solutions, high photostability, and reduced damage to red blood cells, highlighting the importance of PSMA coupling. Furthermore, the PSMA-ICG NPs showed significant tumor targeting and long-term imaging capabilities, as well as potent photothermal therapy when combined with near-infrared laser irradiation. Overall, this study provides promising phototheranostic agents for clinical applications.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Chemistry, Organic
Xuanjun Wu, Hunter McFall-Boegeman, Zahra Rashidijahanabad, Kunli Liu, Christian Pett, Jin Yu, Manuel Schorlemer, Sherif Ramadan, Sandra Behren, Ulrika Westerlind, Xuefei Huang
Summary: A synthetic MUC1-beta-Tf with enhanced stability was developed by altering the stereochemistry of the glycan/peptide linkage, leading to high levels of IgG antibodies in clinically relevant human MUC1 transgenic mice and recognizing MUC1 expressing tumor cells. This suggests a potential strategy for anti-cancer vaccine epitope design for glycopeptides.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Qingyu Zhao, Yanan Gao, Min Xiao, Xuefei Huang, Xuanjun Wu
Summary: Peptide epitopes from the spike protein of SARS-CoV-2 were synthesized and linked with CRM197 to immunize mice, leading to high titers of IgG antibodies that effectively neutralized SARS-CoV-2 pseudovirus. This study suggests that the identified epitopes are protective and the conjugates are promising for the development of an anti-SARS-CoV-2 vaccine.
CHEMICAL COMMUNICATIONS
(2021)
Article
Polymer Science
Shuyao Lang, Zibin Tan, Xuanjun Wu, Xuefei Huang
Article
Chemistry, Medicinal
Shibin Zhao, Julian Maceren, Mia Chung, Samantha Stone, Raphael Geiben, Melissa L. Boby, Bradley S. Sherborne, Derek S. Tan
Summary: Antibiotic resistance is a major threat to public health, with Gram-negative bacteria presenting unique challenges due to their low permeability and efflux pumps. Limited understanding of the chemical rules for overcoming these barriers hinders antibacterial drug discovery. Efforts to address this issue, such as screening compound libraries and using cheminformatic analysis, have led to the design of sulfamidoadenosines with diverse substituents, showing potential utility in accumulation in Escherichia coli.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Jichun Li, Qing Li, Shuai Xia, Jiahuang Tu, Longbo Zheng, Qian Wang, Shibo Jiang, Chao Wang
Summary: This study successfully developed a short peptide mimetic as a MERS-CoV fusion inhibitor by reproducing the key recognition features of the HR2 helix. The resulting 23-mer lipopeptide showed comparable inhibitory effect to the 36-mer HR2 peptide HR2P-M2. This has important implications for developing short peptide-based antiviral agents to treat MERS-CoV infection.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Krista Jaunsleine, Linda Supe, Jana Spura, Sten van Beek, Anna Sandstrom, Jessica Olsen, Carina Halleskog, Tore Bengtsson, Ilga Mutule, Benjamin Pelcman
Summary: Beta(2)-adrenergic receptor agonists can stimulate glucose uptake by skeletal muscle cells and are therefore potential treatments for type 2 diabetes. The chirality of compounds has a significant impact on the activity of these agonists. This study found that certain synthesized compounds showed higher glucose uptake activity. These findings provide important information for the design of novel beta(2)AR agonists for T2D treatment.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Xin Xu, Jia Chen, Guan Wang, Xiaojuan Zhang, Qiang Li, Xiaobo Zhou, Fengying Guo, Min Li
Summary: The study focuses on EZH2, a promising therapeutic target for various types of cancers. Researchers designed and synthesized a series of novel derivatives aiming to enhance the EZH2 inhibition activity. Among them, compound 28 displayed potent EZH2 inhibition activity and showed high anti-proliferative effects in lymphoma cell lines and xenograft mouse models. The study suggests that compound 28 has potential as a therapeutic candidate for EZH2-associated cancers.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Wei Zhang, Wei Liu, Ya-Dong Zhao, Li-Zi Xing, Ji Xu, Rui-Jun Li, Yun-Xiao Zhang
Summary: This study developed a series of aromatic amide derivatives based on Rhein and investigated their inhibitory activity against alpha-Syn aggregation. Two of these compounds showed promising potential in treating Parkinson's disease by stabilizing alpha-Syn's conformation and disassembling alpha-Syn oligomers and fibrils.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Mani Sharma, S. S. S. S. Sudha Ambadipudi, Neeraj Kumar Chouhan, V. Lakshma Nayak, Srihari Pabbaraja, Sai Balaji Andugulapati, Ramakrishna Sistla
Summary: Therapeutically active lipids in drug delivery systems can enhance the safety and efficacy of treatment. The liposome formulation created using synthesized biologically active lipids showed additive anti-cancer effects and reduced tumorigenic potential.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
