Gold nanoparticles conjugated with monosaccharide-modified peptide for lectin detection

Gold nanoparticles (GNPs) conjugated with monosaccharide-modified peptides have been developed as optical probes for lectin detection. Mannose-modified peptides were designed and conjugated with GNPs. The GNPs with mannose-modified peptide showed remarkable red shift of absorption maximum due to the aggregation with concanavalin A (ConA), a mannose-binding lectin. The aggregation activity of glycopeptide-modified GNPs with ConA depended on the amino acid sequence around the mannose unit of glycopeptides. (C) 2012 Elsevier Ltd. All rights reserved.