Article
Biochemistry & Molecular Biology
Freeborn Rwere, Sangchoul Im, Lucy Waskell
Summary: By altering the 140s FMN binding loop of CYPOR, the activity with cyt P450s can be affected, with the mutant activity primarily determined by its ability to reduce ferric cyt P450. The different behaviors of the mutants are mainly due to the altered structure and mobility of the loop.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Narayanan Srividya, Iris Lange, Jana K. Richter, Matthias Wuest, B. Markus Lange
Summary: In this study, the enantioselectivity of multiple enzymes involved in monoterpenoid biosynthesis in peppermint and Japanese catnip was investigated. The experimental results showed that these enzymes exhibited different enantioselectivity towards intermediates of the pathway, and molecular modeling studies supported the experimental data. This study lays the groundwork for further structure-function studies on the origin of enantioselectivity in closely related species of the Lamiaceae.
Article
Biochemistry & Molecular Biology
Manuel Sellner, Andre Fischer, Charleen G. Don, Martin Smiesko
Summary: Research using molecular dynamics simulations and protein-protein docking revealed the mechanism of conformational changes between cytochrome P450 enzymes and cytochrome P450 reductase, as well as proposed new conformational transition mechanisms and structural mechanisms for susceptibility to redox states.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Nataliia Kovalchuk, Joseph L. Jilek, Laura S. Van Winkle, Nathan J. Cherrington, Xinxin Ding
Summary: The study used a lung-Cpr-null mouse model to investigate the role of lung POR in PQ-induced pulmonary fibrosis, finding that CPR plays a critical role in this process.
Article
Chemistry, Analytical
Bankala Krishnarjuna, Sang -Ghoul Im, Thirupathi Ravula, Joseph Marte, Richard J. Auchus, Ayyalusamy Ramamoorthy
Summary: This study reports the successful functional reconstitution of a membrane-bound redox complex in non-ionic inulin-based polymer nanodiscs. The nanodiscs have a higher phase-transition temperature and can align in an externally applied magnetic field. These findings suggest that non-ionic polymer nanodiscs can be used for studying the function and structures of various membrane proteins/complexes.
ANALYTICAL CHEMISTRY
(2022)
Article
Ecology
Xiaoman Zhang, Zhiyong Shi, Cai-qing Yang, Jing Li, Jingze Liu, Ai-bing Zhang
Summary: This study identified a co-orthologous gene, CYP4g15, in insects that originated from different bacteria species, suggesting its functional conservation and ancient origin.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2022)
Article
Biochemistry & Molecular Biology
Natalie Jay, Janie E. McGlohon, Fernando Estrada
Summary: This study investigated the interactions between human Adx and CYP enzymes as well as AdR using protein NMR spectroscopy. The results suggested that monomeric Adx forms a functional complex with CYP enzymes in a 1:1 ratio, while Adx dimers may regulate access to monomeric Adx. These findings are important for revising the model of the Adx electron shuttle mechanism.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Melina Lopez, Pedro F. Malacarne, Anna Gajos-Draus, Xinxin Ding, Andreas Daiber, Jon O. Lundberg, Stefan Offermanns, Ralf P. Brandes, Flavia Rezende
Summary: The study suggests that metabolism by vascular CYPs does not contribute to the pharmacological function of organic nitrates, while ALDH2 plays an important role in activating organic nitrates.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Justin C. Miller, Allison J. Hollatz, Mary A. Schuler
Summary: The study identifies differences in the terpene indole alkaloid (TIA) pathways in Catharanthus roseus and Camptotheca acuminata, and demonstrates the ability of specific P450 proteins to convert certain compounds in these pathways. The research provides valuable tools for further examination of substrate recognition and the unique mechanism of C-C bond scission involved in these pathways.
Review
Oncology
Ewelina Janczy-Cempa, Olga Mazuryk, Agnieszka Kania, Malgorzata Brindell
Summary: This review provides an overview of the current knowledge about the expression and activity of oxidoreductases relevant to the activation of HAPs and fluorescent imaging probes. The low efficacy of HAPs in clinical trials may be attributed to the lack of screening for tumor hypoxia and limited availability of specific oxidoreductases. Furthermore, there is insufficient understanding of the expression and activity of these enzymes in various cancer cell lines under hypoxic conditions, highlighting the need for further systematic studies. The review also discusses the use of fluorescence probes triggered by reduction with specific oxidoreductases for imaging hypoxic cancer cells.
Article
Biochemistry & Molecular Biology
J. Patrick Connick, James R. Reed, George F. Cawley, Aratrika Saha, Wayne L. Backes
Summary: The goal of this study was to characterize the ability of CYP1 proteins to form protein-protein complexes, their localization in lipid microdomains, and their overall function. The study found significant differences in their quaternary structures and function, despite their high sequence similarity, substrate selectivities, and induction characteristics.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Chemistry, Medicinal
Soudeh Bahramian Nasab, Ahmad Homaei, Roberto Fernandez-Lafuente, Jon Del Arco, Jesus Fernandez-Lucas
Summary: The use of marine enzymes as catalysts for biotechnological applications is a hot topic. In this study, a liver NADPH-dependent cytochrome P450 reductase (CPR) from the marine fish Liza klunzingeri (LkCPR) was isolated, purified and biochemically characterized. LkCPR showed remarkable catalytic features, such as a wide range of pH and temperature stability, high catalytic activity, and highest thermostability among reported CPRs. These findings suggest that LkCPR has great potential as a biocatalyst.
Article
Chemistry, Multidisciplinary
Mingle Li, Kalayou Hiluf Gebremedhin, Dandan Ma, Zhongji Pu, Tao Xiong, Yunjie Xu, Jong Seung Kim, Xiaojun Peng
Summary: The article introduces a conditionally activatable photoredox catalysis method, which achieves more effective tumor treatment through site-specific photoredox. This approach enables control of photoresponsivity while ensuring biocompatibility and safety.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biology
Jiemin Shen, Gang Wu, Ah-Lim Tsai, Ming Zhou
Summary: This study demonstrates the formation of stable binary and ternary complexes between mammalian cytochrome b(5), cytochrome b(5) reductase, and stearoyl-CoA desaturase-1, which enhance electron transfer rates.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ru-Yi Ye, Juan Song, Zhi-Jun Zhang, Hui-Lei Yu
Summary: CYP120As are unique non-membrane P450 enzymes that play a significant role in the biodegradation of retinoids, with promising catalytic potential.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Medicinal
Shibin Zhao, Julian Maceren, Mia Chung, Samantha Stone, Raphael Geiben, Melissa L. Boby, Bradley S. Sherborne, Derek S. Tan
Summary: Antibiotic resistance is a major threat to public health, with Gram-negative bacteria presenting unique challenges due to their low permeability and efflux pumps. Limited understanding of the chemical rules for overcoming these barriers hinders antibacterial drug discovery. Efforts to address this issue, such as screening compound libraries and using cheminformatic analysis, have led to the design of sulfamidoadenosines with diverse substituents, showing potential utility in accumulation in Escherichia coli.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Jichun Li, Qing Li, Shuai Xia, Jiahuang Tu, Longbo Zheng, Qian Wang, Shibo Jiang, Chao Wang
Summary: This study successfully developed a short peptide mimetic as a MERS-CoV fusion inhibitor by reproducing the key recognition features of the HR2 helix. The resulting 23-mer lipopeptide showed comparable inhibitory effect to the 36-mer HR2 peptide HR2P-M2. This has important implications for developing short peptide-based antiviral agents to treat MERS-CoV infection.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Krista Jaunsleine, Linda Supe, Jana Spura, Sten van Beek, Anna Sandstrom, Jessica Olsen, Carina Halleskog, Tore Bengtsson, Ilga Mutule, Benjamin Pelcman
Summary: Beta(2)-adrenergic receptor agonists can stimulate glucose uptake by skeletal muscle cells and are therefore potential treatments for type 2 diabetes. The chirality of compounds has a significant impact on the activity of these agonists. This study found that certain synthesized compounds showed higher glucose uptake activity. These findings provide important information for the design of novel beta(2)AR agonists for T2D treatment.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Xin Xu, Jia Chen, Guan Wang, Xiaojuan Zhang, Qiang Li, Xiaobo Zhou, Fengying Guo, Min Li
Summary: The study focuses on EZH2, a promising therapeutic target for various types of cancers. Researchers designed and synthesized a series of novel derivatives aiming to enhance the EZH2 inhibition activity. Among them, compound 28 displayed potent EZH2 inhibition activity and showed high anti-proliferative effects in lymphoma cell lines and xenograft mouse models. The study suggests that compound 28 has potential as a therapeutic candidate for EZH2-associated cancers.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Wei Zhang, Wei Liu, Ya-Dong Zhao, Li-Zi Xing, Ji Xu, Rui-Jun Li, Yun-Xiao Zhang
Summary: This study developed a series of aromatic amide derivatives based on Rhein and investigated their inhibitory activity against alpha-Syn aggregation. Two of these compounds showed promising potential in treating Parkinson's disease by stabilizing alpha-Syn's conformation and disassembling alpha-Syn oligomers and fibrils.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Mani Sharma, S. S. S. S. Sudha Ambadipudi, Neeraj Kumar Chouhan, V. Lakshma Nayak, Srihari Pabbaraja, Sai Balaji Andugulapati, Ramakrishna Sistla
Summary: Therapeutically active lipids in drug delivery systems can enhance the safety and efficacy of treatment. The liposome formulation created using synthesized biologically active lipids showed additive anti-cancer effects and reduced tumorigenic potential.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)