Journal
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Volume 21, Issue 5, Pages 1359-1362Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2011.01.038
Keywords
Target protein; Immunoprecipitation; Molecular probe; Linker
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Funding
- MEXT [20310128]
- Asahi-Glass Foundation
- TOREY Foundation
- Naito Foundation
- Grants-in-Aid for Scientific Research [23310147, 20310128, 23102012] Funding Source: KAKEN
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Affinity purification using immunoprecipitation (IP) is an extremely useful method for target profiling of bioactive natural products. We examined IP purification of CMetE, which is a molecular target for potassium isolespedezate (1), a leaf-opening factor of Cassia plant. We studied IP efficiency using a panel of FLAG-connected molecular probes (2-8), including probes with varying structures and lengths of the linker moiety. The results suggest that not only the length, but the chemical nature of the linker moiety, strongly affect the IP efficiency. 3XFLAG, a tag combined with a linker moiety of charged amino acids, gave the best results and was most useful for IP purification of the molecular target. (C) 2011 Elsevier Ltd. All rights reserved.
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