Article
Plant Sciences
Magda F. El-Adl, Taha M. El-Katony, Reham M. Nada
Summary: The study found that the specific ion effects of Na+ and K+ were different on the growth of Ulva lactuca. Ulva lactuca showed better growth with high concentrations of NaCl, while KCl might have adverse effects on photosynthesis and cell wall. Transcription of PM H+-ATPase was induced specifically by high concentrations of Na+, while the induction of PM-Na+/K+-ATPase-like alone could not improve the growth of KCl-treated Ulva lactuca.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Plant Sciences
Qi Chen, Kai Xu, Yan Xu, Dehua Ji, Changsheng Chen, Chaotian Xie, Wenlei Wang
Summary: This study analyzed the function of Na+/K+-ATPase in the response of Neoporphyra haitanensis to salt stress and found that it played a crucial role in maintaining the K+/Na+ homeostasis in the algae. The inhibitor ouabain caused Na+ accumulation and K+ leakage in the cells, disrupting the K+/Na+ balance and leading to decreased photosynthesis and oxidative damage in the algae.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Marlene U. Fruergaard, Ingrid Dach, Jacob L. Andersen, Mette Ozol, Azadeh Shahsavar, Esben M. Quistgaard, Hanne Poulsen, Natalya U. Fedosova, Poul Nissen
Summary: In this study, the crystal structure and functional properties of the E2-BeFx complex of pig kidney Na+,K+-ATPase are investigated. The complex resembles the E2P intermediate and is able to bind ADP/ATP and Na+. The results suggest a relation between E2-BeFx and the E*P intermediate in the functional cycle of Na+, K+-ATPase.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Vijay Joshi, Arianne Penalosa, Madhumita Joshi, Sierra Rodriguez
Summary: Transcriptomic analysis revealed a complex network of genes regulating oxalate homeostasis in spinach, with molecular functions associated with enzymatic activities and enrichment of metabolic pathways. Genes associated with distinct physiological processes collectively regulate oxalate, providing novel insights into oxalate metabolism in spinach.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Santiago E. Faraj, Wanda M. Valsecchi, Mariela Ferreira-Gomes, Mercedes Centeno, Elina Malen Saint Martin, Natalya U. Fedosova, Juan Pablo FC. Rossi, Monica R. Montes, Rolando C. Rossi
Summary: The Na+/K+-ATPase is a plasma membrane glycoprotein that exchanges intracellular Na+ for extracellular K+ by hydrolyzing ATP. The asymmetric distribution of Na+ and K+ is essential for cellular life. Current research indicates that Na+ becomes spontaneously occluded in the E1 dephosphorylated form of the Na+/K+-ATPase, showing positive interactions between binding sites. Adding ATP does not increase Na+ occlusion.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Review
Microbiology
Cassiano Felippe Goncalves-de-Albuquerque, Carolina Medina Coeli da Cunha, Leo Victor Grimaldi de Castro, Caroline de Azevedo Martins, Marcos Roberto Colombo Barnese, Patricia Burth, Mauricio Younes-Ibrahim
Summary: In 1915, Inada and Ido identified Leptospira sp. as the pathogen responsible for Weil's Disease, which was later confirmed to cause leptospirosis. The molecular mechanisms and interactions between bacterial components and the host, as well as the systemic dysfunctions, still need to be fully understood. Leptospirosis affects all animal species and involves a cellular mechanism common to all eukaryotes. Na/K-ATPase is targeted by the leptospiral endotoxin, leading to dysfunctions and organ disorders. Additionally, metabolic disorders and inflammatory responses are triggered during leptospirosis, involving various molecular mechanisms. Na/K-ATPase plays a critical role in the disease and can be a potential therapeutic target or an indicator of disease severity.
Article
Cell Biology
Bruno Sterlini, Alessandra Romei, Chiara Parodi, Davide Aprile, Michele Oneto, Anita Aperia, Pierluigi Valente, Flavia Valtorta, Anna Fassio, Pietro Baldelli, Fabio Benfenati, Anna Corradi
Summary: This study reveals that PRRT2 deficiency affects the function of Na+/K+ ATPase (NKA) in neurons, leading to increased clustering of NKA on the plasma membrane, which can be reversed by re-expression of PRRT2. Additionally, PRRT2 deficiency also reduces the NKA-dependent afterhyperpolarization in neurons following high-frequency firing.
CELL DEATH & DISEASE
(2021)
Article
Cell Biology
Rawad Hodeify, Sawsan Kreydiyyeh, Leen Mohammad Jamal Zaid
Summary: The sodium-potassium pump (NKA) consumes a significant amount of energy in animal cells for creating electrochemical gradients of sodium and potassium ions. The regulation of its activity, including its expression, localization, enzymatic activity, and interactions with other proteins, is crucial. However, the mechanisms controlling the trafficking of NKA between the cell membrane and intracellular compartments are poorly understood. This review summarizes previous studies on the regulation of NKA trafficking and its potential endocytic signals, as well as highlights recent research on NKA trafficking and modulation in various cells.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Parasitology
Xiao-Fei Shang, Xiao-Lou Miao, Li-Xia Dai, Yu Wang, Bing Li, Hu Pan, Ji-Yu Zhang
Summary: This study confirmed the strong acaricidal activity and inhibitory effect on Na+-K+-ATPase of the novel strophanthidin derivative QXG-1. By chemically modifying specific positions of the strophanthidin molecule, its toxicity against mites can be controlled. The safety of QXG-1 in vitro and in vivo also suggests its potential as a promising acaricidal agent.
VETERINARY PARASITOLOGY
(2021)
Article
Immunology
Jin Zheng, Ping Lan, Meihe Li, Min-chao Kang, Meng Xun, Xiangyun Ma, Mengyao Yan, Dan Sun, Yu Shen, Xinyi Fu, Xiaoming Ding, Xiaofei Yan, Wu-jun Xue
Summary: Reduced Na+/K+-ATPase (NKA) activity and NKA alpha 1 expression are implicated in the pathogenesis of renal diseases. NKA alpha 1 deficiency exacerbates tubulointerstitial lesions and fibrosis in a mouse model of UUO. Activation of NKA alpha 1 by an antibody against its extracellular DR region (DRm217) prevents UUO-induced tubulointerstitial lesions, preserves kidney function, and reduces renal fibrosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Chemistry, Medicinal
Lucy Kate Ladefoged, Birgit Schiott, Natalya U. Fedosova
Summary: The study reveals significant differences in the binding properties of different subfamilies of cardiotonic steroids with the Na+,K+-ATPase. While bufadienolides show beneficial effects with K+, they differ from cardenolides which have an antagonistic relationship with K+. Through simulations, it was found that the binding of bufadienolides is influenced by amino acid coordination patterns and the presence of cations, illustrating the complex interplay of factors in these interactions.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2021)
Article
Biochemistry & Molecular Biology
Aline Yammine, Lizette Auezova, Gerard Lizard, Helene Greige-Gerges
Summary: The aim of this study was to investigate whether quercetin, resveratrol, or caffeic acid, at nano-and low micromolar concentrations, can improve the activity of Na+/K+- and Ca2+-ATPases in human erythrocyte membranes with excess cholesterol. These polyphenols belong to different chemical classes and are widely present in plant foods.
Article
Food Science & Technology
Zena Wehbe, Sawsan Kreydiyyeh
Summary: The increase in bovine dairy consumption has been associated with the development of inflammatory diseases. Milk exosomes, which contain cytokines elevated in inflammatory bowel disease, are potential causative agents. This study investigates the relationship between exosomes and Na+/K+ ATPase using Caco-2 cells. The exosomes were found to dose and time-dependently increase ATPase activity, mediated by PGE2 and involving EP3 and EP4 receptors. The activation of ATPase was influenced by PKA and affected by EP3 and EP4 action.
Article
Multidisciplinary Sciences
Victoria C. Young, Hanayo Nakanishi, Dylan J. Meyer, Tomohiro Nishizawa, Atsunori Oshima, Pablo Artigas, Kazuhiro Abe
Summary: This study investigates ion transport mechanisms by introducing mutations to convert an electroneutral proton/potassium pump into a prototypical electrogenic sodium/potassium pump, explaining their selectivity and phosphorylation mechanisms.
NATURE COMMUNICATIONS
(2022)
Article
Biology
R. Meldrum Robertson, Christopher D. Moyes
Summary: The study shows that rapid cold hardening provides protection for locusts by activating Na+/K+-ATPase, enhancing in situ NKA activity in the nervous system, as well as affecting other neuronal properties that promote visual processing.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2022)
Article
Chemistry, Medicinal
Shibin Zhao, Julian Maceren, Mia Chung, Samantha Stone, Raphael Geiben, Melissa L. Boby, Bradley S. Sherborne, Derek S. Tan
Summary: Antibiotic resistance is a major threat to public health, with Gram-negative bacteria presenting unique challenges due to their low permeability and efflux pumps. Limited understanding of the chemical rules for overcoming these barriers hinders antibacterial drug discovery. Efforts to address this issue, such as screening compound libraries and using cheminformatic analysis, have led to the design of sulfamidoadenosines with diverse substituents, showing potential utility in accumulation in Escherichia coli.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Jichun Li, Qing Li, Shuai Xia, Jiahuang Tu, Longbo Zheng, Qian Wang, Shibo Jiang, Chao Wang
Summary: This study successfully developed a short peptide mimetic as a MERS-CoV fusion inhibitor by reproducing the key recognition features of the HR2 helix. The resulting 23-mer lipopeptide showed comparable inhibitory effect to the 36-mer HR2 peptide HR2P-M2. This has important implications for developing short peptide-based antiviral agents to treat MERS-CoV infection.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Krista Jaunsleine, Linda Supe, Jana Spura, Sten van Beek, Anna Sandstrom, Jessica Olsen, Carina Halleskog, Tore Bengtsson, Ilga Mutule, Benjamin Pelcman
Summary: Beta(2)-adrenergic receptor agonists can stimulate glucose uptake by skeletal muscle cells and are therefore potential treatments for type 2 diabetes. The chirality of compounds has a significant impact on the activity of these agonists. This study found that certain synthesized compounds showed higher glucose uptake activity. These findings provide important information for the design of novel beta(2)AR agonists for T2D treatment.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Xin Xu, Jia Chen, Guan Wang, Xiaojuan Zhang, Qiang Li, Xiaobo Zhou, Fengying Guo, Min Li
Summary: The study focuses on EZH2, a promising therapeutic target for various types of cancers. Researchers designed and synthesized a series of novel derivatives aiming to enhance the EZH2 inhibition activity. Among them, compound 28 displayed potent EZH2 inhibition activity and showed high anti-proliferative effects in lymphoma cell lines and xenograft mouse models. The study suggests that compound 28 has potential as a therapeutic candidate for EZH2-associated cancers.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Wei Zhang, Wei Liu, Ya-Dong Zhao, Li-Zi Xing, Ji Xu, Rui-Jun Li, Yun-Xiao Zhang
Summary: This study developed a series of aromatic amide derivatives based on Rhein and investigated their inhibitory activity against alpha-Syn aggregation. Two of these compounds showed promising potential in treating Parkinson's disease by stabilizing alpha-Syn's conformation and disassembling alpha-Syn oligomers and fibrils.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Mani Sharma, S. S. S. S. Sudha Ambadipudi, Neeraj Kumar Chouhan, V. Lakshma Nayak, Srihari Pabbaraja, Sai Balaji Andugulapati, Ramakrishna Sistla
Summary: Therapeutically active lipids in drug delivery systems can enhance the safety and efficacy of treatment. The liposome formulation created using synthesized biologically active lipids showed additive anti-cancer effects and reduced tumorigenic potential.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)