Journal
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Volume 20, Issue 7, Pages 2215-2218Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2010.02.025
Keywords
Amphidinol; Surface plasmon resonance; Cholesterol; Ergosterol
Categories
Funding
- MEXT, Japan [18101010, 16073211, 17681027]
- Naito Foundation
- A Monbukagakusho scholarship
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The affinity of amphidinol 3 (AM3) to phospholipid membranes in the presence and absence of sterol was examined by surface plasmon resonance (SPR) experiments. The results showed that AM3 has 1000 and 5300 times higher affinity for cholesterol-and ergosterol-containing liposomes, respectively, than those without sterol. The two-state reaction model well reproduced the sensor grams, which indicated that the interaction is composed of two steps, which correspond to binding to the membrane and internalization to form stable complexes. (C) 2010 Elsevier Ltd. All rights reserved.
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