Journal
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Volume 19, Issue 4, Pages 1258-1260Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2008.12.090
Keywords
Kinase assay; Fluorescence; Library screening
Categories
Funding
- NIH Cell Migration Consortium [GM064346]
- Invitrogen Corporation
- A. M. Escudero Foundation
- Biophysical Instrumentation Facility for the Study of Complex Macromolecular Systems [NSF-0070319]
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A novel screening method to identify selective Sox-based fluorescent probes for Ser/Thr kinases has been developed. Peptide libraries were exposed to a kinase of interest and the products of the timed reaction were analyzed by MALDI-TOF. To demonstrate the potential of this methodology, a selective substrate for Aurora A kinase was identified that showed a 7-fold improvement in catalytic efficiency over the best substrate described to date in the literature. (C) 2008 Elsevier Ltd. All rights reserved.
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