Article
Biochemistry & Molecular Biology
Vasantharaja Raguraman, Leena Chauhan, Priyanka Gehlot, Bhani Kongkham, P. Hariprasad
Summary: In this study, 150 plant secondary metabolites (PSMs) with the lowest binding energy were identified through molecular docking. These PSMs belong to chemical classes such as tannins, flavonoid glycosides, terpene glycosides, steroid lactones, etc. Bismahanine, Ashwagandhanolide, and Daurisoline formed stable complexes with IGPD and showed strong H-bond interactions with specific amino acid residues. These PSMs could be potential leads against IGPD and should be further investigated in vitro and in vivo.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Biotechnology & Applied Microbiology
Meng Zhang, Liyuan Kong, Rong Gong, Marianna Iorio, Stefano Donadio, Zixin Deng, Margherita Sosio, Wenqing Chen
Summary: This review summarizes recent advances in the biosynthesis of C-nucleoside antibiotics and tentatively discusses future developments in accessing C-nucleoside diversities through synthetic biology strategies.
MICROBIAL CELL FACTORIES
(2022)
Review
Biochemical Research Methods
Natesh Singh, Ludovic Chaput, Bruno O. Villoutreix
Summary: The interaction between life sciences and advancing technology leads to continuous growth of chemical data, with virtual screening methods being popular in pharmaceutical research. Web-based tools assist scientists in conducting virtual screening experiments, contributing to the design of bioactive molecules and drug development teaching.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Plant Sciences
Qiyao Shen, Guangzhi Dai, Vinothkannan Ravichandran, Yang Liu, Lin Zhong, Haiyan Sui, Xiangmei Ren, Nianzhi Jiao, Youming Zhang, Haibo Zhou, Xiaoying Bian
Summary: Siderophores are secreted by microorganisms to survive in iron-depleted conditions and have therapeutic potential. Eight amphiphilic siderophores, saccharochelins A-H, were identified from a rare marine-derived Saccharothrix species and showed cytotoxicity against human tumor cell lines. The fatty acid side chains of saccharochelins significantly affected cytotoxicity, indicating potential for producing more potent derivatives.
JOURNAL OF NATURAL PRODUCTS
(2021)
Review
Microbiology
Xiangyang Ji, Wan-Qiu Liu, Jian Li
Summary: Natural products with complex chemical structures and diverse bioactivities can be produced rapidly and sustainably using cell-free systems, which reconstitute their entire biosynthetic pathways in vitro. This technology holds tremendous potential for various applications in natural product research.
CURRENT OPINION IN MICROBIOLOGY
(2022)
Article
Chemistry, Organic
Graham A. Hudson, Annie R. Hooper, Adam J. DiCaprio, David Sarlah, Douglas A. Mitchell
Summary: The study successfully identified and synthesized a new class of natural products, named pyritides, using a combination of bioinformatics, enzymology, and synthetic methods. These pyritides have a unique structure distinct from thiopeptides, with predicted enzymatic reactions leading to the formation of a trisubstituted pyridine-based macrocycle. The research demonstrates the potential of integrating bioinformatics, enzymology, and synthesis to characterize novel natural product scaffolds with interesting structural features and biological activities.
Article
Food Science & Technology
Xiyu Duan, Hui Yang, Chong Wang, Huhu Liu, Xiangyang Lu, Yun Tian
Summary: This review provides a comprehensive analysis of the microbial resources of cordycepin and the biosynthesis pathway in artificial cultured cordycepin-producing strains. The methods of strain improvement, genetic engineering, and synthetic biology for increasing cordycepin production are also discussed.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2023)
Article
Biology
Abhisek Dwivedy, Anam Ashraf, Bhavya Jha, Deepak Kumar, Nisheeth Agarwal, Bichitra K. Biswal
Summary: Intracellular pathogens such as Mycobacterium tuberculosis have developed strategies to evade host immune responses and meet their nutritional requirements, even in the presence of immune pressure. Host cells can up-regulate histidine catabolizing enzymes to starve the pathogen of essential amino acids, but the pathogen can overcome this by de novo synthesis. This study sheds light on the importance of histidine biosynthesis for the pathogenesis of Mtb and reveals a potential link between histidine metabolism and Mtb infection.
COMMUNICATIONS BIOLOGY
(2021)
Review
Biochemical Research Methods
Dustin J. Vollmann, Lea Winand, Markus Nett
Summary: Natural products play a significant role in drug development, often enhanced in activity and reduced in toxicity through modification or derivation. Current research focuses on integrating organic synthesis and biosynthesis through hybrid processes.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Chemistry, Organic
Atina Rizkiya Choirunnisa, Kuga Arima, Yo Abe, Noritaka Kagaya, Kei Kudo, Hikaru Suenaga, Junko Hashimoto, Manabu Fujie, Noriyuki Satoh, Kazuo Shin-ya, Kenichi Matsuda, Toshiyuki Wakimoto
Summary: This study identified four new analogs of aliphatic azoxides through a reactivity-based screening. The study found that the double bond in the alkyl side chain is crucial for the cytotoxicity of azodyrecins. Additionally, the study elucidated the tailoring step of azodyrecin biosynthesis.
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Huayue Li, Xiao Han, Yujing Dong, Shanshan Xu, Chao Chen, Yingang Feng, Qiu Cui, Wenli Li
Summary: Bacillaenes, a class of poly-unsaturated enamines produced by Bacillus strains, are found to play dual roles as antibiotics and biofilm enhancers. This study reveals the instability of bacillaenes and their potential for stabilizing the scaffold through chemical modification. The findings suggest their importance in self-protection of Bacillus through accelerated biofilm formation in a dose-dependent manner.
Article
Chemistry, Multidisciplinary
Ryuhei Nagata, Hironori Suemune, Masaya Kobayashi, Tetsuro Shinada, Kazuo Shin-ya, Makoto Nishiyama, Tomoya Hino, Yusuke Sato, Tomohisa Kuzuyama, Shingo Nagano
Summary: This study elucidated the crystal structures of a new subclass of aromatic substrate PTs, and highlighted the essential roles of Ile and Asp residues in interactions with prenyl acceptors. A novel deprotonation mechanism involving a catalytic triad for aromatic substrate PTs was proposed based on structural and mutational analyses.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biotechnology & Applied Microbiology
E. Yang, Jun Meng, Heqing Cai, Caibin Li, Sainan Liu, Luming Sun, Yanxiang Liu
Summary: The organic compounds found in biochar act as hormone analogs, stimulating the expression of metabolites by controlling related genes and proteins to promote L-histidine biosynthesis in E. coli. The optimal concentration was determined to be 3%, with 7% being lethal. Docking technology was used to better understand the mechanisms, with GC-MS analysis revealing the connection between three organic molecules and HisG protein in regulating L-histidine biosynthesis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Cedric Couturier, Sebastian Gross, Alexander von Tesmar, Judith Hoffmann, Selina Deckarm, Anouchka Fievet, Nelly Dubarry, Thomas Taillier, Christoph Poeverlein, Heike Stump, Michael Kurz, Luigi Toti, Sabine Haag Richter, Dietmar Schummer, Philippe Sizun, Michael Hoffmann, Ram Prasad Awal, Nestor Zaburannyi, Kirsten Harmrolfs, Joachim Wink, Emilie Lessoud, Thierry Vermat, Veronique Cazals, Sandra Silve, Armin Bauer, Michael Mourez, Laurent Fraisse, Corinne Leroi-Geissler, Astrid Rey, Stephanie Versluys, Eric Bacque, Rolf Mueller, Stephane Renard
Summary: This article describes the myxobacterial natural product Corramycin isolated from Corallococcus coralloides. Corramycin exhibits anti-Gram-negative activity against Escherichia coli and is taken up via two transporter systems. The biosynthetic gene cluster (BGC) of Corramycin was identified and a biosynthesis model was proposed. The absolute configuration of the molecule was elucidated through bioinformatic analysis and total synthesis. Animal experiments confirmed the antibacterial effect of Corramycin.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Yalong Zhang, Jian Bai, Le Zhang, Chen Zhang, Bingyu Liu, Youcai Hu
Summary: A unique self-resistance mechanism for fungal antibiotics was discovered, utilizing an oxidative-reductive cycle to prevent self-harm. This work contributes to the understanding of antibiotic biosynthesis and resistance mechanisms.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)