Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 407, Issue 18, Pages 5273-5280Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-015-8741-z
Keywords
Multiple myeloma; Phosphatidylcholine; Single cell; Matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS)
Funding
- Japan Society for the Promotion of Science [25293044]
- Ministry of Education, Culture, Sports, Science and Technology [25116712]
- Ministry of Health, Labor, and Welfare [H23-Iryoukiki-Ippan-001]
- Grants-in-Aid for Scientific Research [25116712, 25293044, 15H05897] Funding Source: KAKEN
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Lipid metabolic changes under diseased conditions, particularly in solid tumors, are attracting increased attention. However, in non-solid tumors, including most hematopoietic tumors, lipid analyses are scarce. Multiple myeloma (MM) is a plasma cell disorder arising from bone marrow, and the lipid status of MM cells has not been reported yet. In this study, we analyzed flow cytometry-sorted single MM cells and normal plasma cells (NPCs) using matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS), a two-dimensional label-free mass spectrometry technique for biomolecular analysis, to obtain specific lipid information. We isolated 1.31-5.77 % of MM cells and 0.03-0.24 % of NPCs using fluorescence-activated cell sorting (FACS). Analysis of purified cells using MALDI-IMS at the single-cell level revealed that the peak intensity and ion signals of phosphatidylcholine [PC (16:0/20:4) + H](+) at m/z 782.5 were significantly decreased in MM cells compared to NPCs. By examining particular cell populations rather than cell mixtures, our method can become a suitable tool for the analysis of rare cell populations at the single-cell level and advance the understanding of MM progression.
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