Article
Microbiology
Hang Xiao, Qin Zhao, Jianlin Yuan, Wei Liang, Rui Wu, Yiping Wen, Senyan Du, Yiping Wang, Shan Zhao, Yifei Lang, Qigui Yan, Xiaobo Huang, Sanjie Cao
Summary: In this study, it was found that IFN-γ plays an important role in regulating Pasteurella multocida toxin-associated pneumonia. IFN-γ deficiency alleviates the severity of pneumonia, increases the survival rate, and reduces lung pathology. Additionally, IFN-γ deficiency decreases myeloperoxidase expression and impairs PANoptosis activation markers, including caspase 3, GSDMD, and MLKL, as well as reduces IL-1 beta expression.
VETERINARY MICROBIOLOGY
(2023)
Article
Immunology
Yajuan Li, Junfang Xiao, Yung-Fu Chang, Hui Zhang, Yutao Teng, Wencheng Lin, Hongxin Li, Weiguo Chen, Xinheng Zhang, Qingmei Xie
Summary: This study successfully cloned and expressed three proteins of the duck cholera pathogen, and formulated subunit and killed vaccines. The results demonstrated that the subunit vaccines generated significant antibody responses and provided varying degrees of protection. These findings indicate the potential of recombinant PlpE or OmpH fusion proteins as vaccine candidates for duck cholera.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Veterinary Sciences
Min-Chia Wu, Hsing-Chieh Wu, Jai-Wei Lee, Wan-Chen Chang, Chun-Yen Chu
Summary: This study successfully enhanced the immune response and protection against P. multocida in piglets by using recombinant Sly and CpG oligodeoxynucleotides as adjuvants. The combination of protein-based antigen with a biological adjuvant showed synergistic effects and protection against atrophic rhinitis, and has the potential to be developed as part of a bivalent vaccine.
VETERINARY RESEARCH
(2023)
Article
Veterinary Sciences
Min-Chia Wu, Yi-Ting Lo, Hsing-Chieh Wu, Hsian-Yu Wang, Chun-Yen Chu
Summary: The study expressed different fragments of recombinant PMT proteins, combined with a water-in-oil-in-water adjuvant, and evaluated mice's immune response. The rPMT-C-immunized group showed significantly higher levels of IgG, IgG2a antibody, interferon-gamma, and IL-12 cytokine expression than other groups. Vaccination with rPMT-C recombinant protein can provide homologous and heterologous protection against P. multocida challenge.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Sanghyeon Choi, Byoung Sik Kim, Jungwon Hwang, Myung Hee Kim
Summary: Opportunistic bacteria strategically dampen their virulence to survive and propagate in hosts, but the molecular mechanisms underlying virulence control are not clearly understood. The opportunistic pathogen Vibrio vulnificus biotype 3 secretes significantly less virulent MARTX toxin, which attenuates release of effectors and reduces virulence in cells and in mice. This virulence mechanism is shared in all biotype 3 strains, providing new insights into how opportunistic bacteria persist in an environmental reservoir and prolong the potential to cause outbreaks.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Veterinary Sciences
Min -Chia Wu, Thu-Dung Doan, Jai-Wei Lee, Yi-Ting Lo, Hsing-Chieh Wu, Chun -Yen Chu
Summary: The study demonstrates the potential of suilysin (Sly) from Streptococcus suis as a vaccine adjuvant to enhance immune responses and protective efficacy. In mouse experiments, vaccine formulations using Sly as an adjuvant exhibited increased antibody production, cellular immunity, and survival rate compared to other adjuvants.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Nanoscience & Nanotechnology
Heng Lu, Huanhuan Shen, Yong Huang
Summary: This study investigated the molecular mechanism by which the recombinant Bartonella toxin rPMT damages PK15 cells. The study found that rPMT significantly affected PK15 cells and induced apoptosis. Transcriptome sequencing data showed that rPMT injury upregulated the expression of inflammation-related pathways and genes in PK15 cells. These findings shed light on the molecular mechanisms underlying rPMT-induced cellular damage and its potential role in inflammation-related pathways.
SCIENCE OF ADVANCED MATERIALS
(2023)
Article
Medical Laboratory Technology
Haruno Yoshida, Jung -Min Kim, Takahiro Maeda, Mieko Goto, Yuzo Tsuyuki, Sachiko Shibata, Kenichi Shizuno, Katsuko Okuzumi, Jae-Seok Kim, Takashi Takahashi
Summary: This study compared the virulence factors (VFs) between Pasteurella canis and Pasteurella multocida, and identified the specific prevalence of toxin genes in clinical isolates of P. canis. These toxin genes may serve as promising targets for the rapid identification of P. canis in clinical settings.
ANNALS OF LABORATORY MEDICINE
(2023)
Article
Nanoscience & Nanotechnology
Heng Lu, Huanhuan Shen, Yong Huang
Summary: In this study, the molecular mechanism by which the recombinant multicidal Bartonella toxin rPMT damages PK15 cells was investigated. It was found that rPMT induced apoptosis in PK15 cells and upregulated inflammation-related pathways and gene expression. These findings shed light on the molecular mechanisms underlying rPMT-induced cellular damage.
SCIENCE OF ADVANCED MATERIALS
(2023)
Article
Immunology
Yue Zhang, Lin Lin, Jie Yang, Qingjie Lv, Mixue Wang, Fei Wang, Xi Huang, Lin Hua, Xiangru Wang, Huanchun Chen, Brenda A. Wilson, Bin Wu, Zhong Peng
Summary: This study successfully expressed fragments of Pasteurella multocida toxin in an attenuated strain of Bordetella bronchiseptica, generating single-component mucosal vaccine candidates. Vaccination with these engineered strains induced immune responses and provided protection against Bordetella bronchiseptica and Pasteurella multocida toxin in a mouse model.
Article
Immunology
Wei Liang, Hang Xiao, Jia-Yong Chen, Yung-Fu Chang, San-Jie Cao, Yi-Ping Wen, Rui Wu, Sen-Yan Du, Qi-Gui Yan, Xiao-Bo Huang, Qin Zhao
Summary: A multi-epitope recombinant antigen of PMT (rPMT) showed excellent immunogenicity and protection in a mouse model. Immunization with rPMT stimulated elevated immune responses and reduced lung tissue damage in challenged mice, resulting in improved survival rates. These findings suggest that rPMT has potential as a subunit vaccine candidate against P. multocida infection.
Article
Biochemistry & Molecular Biology
Harper Smith, Nick Pinkerton, David B. Heisler, Elena Kudryashova, Aaron R. Hall, Kelly R. Karch, Andrew Norris, Vicki Wysocki, Marcos Sotomayor, Emil Reisler, Dimitrios Vavylonis, Dmitri S. Kudryashov
Summary: Actin is a crucial element of the immune system and a target for bacterial toxins, with pathogens injecting ACD toxin into host cells to disrupt actin regulation. Linear AOs can be cross-linked to form cyclic AOs, which exhibit reduced toxicity and are less susceptible to host cell neutralization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Kumaraguruparan Ramasamy, Sowmya Balasubramanian, Alejandra Kirkpatrick, Daniel Szabo, Lavanya Pandranki, Joel B. Baseman, T. R. Kannan
Summary: Mycoplasma pneumoniae is a common cause of pneumonia and other respiratory disorders, with its virulence factor CARDS toxin requiring an acidic environment and retrograde transport through the Golgi complex to induce formation of cell vacuoles.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Chelsea D. Landon, Gabriella Dancourt, Vivian Shing, Herman F. Staats
Summary: Rabbits are an excellent model for studying maternal immunization due to similarities in placentation and antibody transfer with humans. Advantages of this research model include ease of breeding and sample collection, short gestation period, and large litter sizes. Commonly assessed routes of immunization include subcutaneous, intramuscular, intranasal, and intradermal.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Review
Chemistry, Multidisciplinary
Dehua Pei
Summary: This paper introduces a previously unrecognized membrane translocation mechanism called the vesicle budding-and-collapse (VBC) mechanism. Through VBC, biomolecules can autonomously translocate across cell membranes. The study found that CPPs and bacterial protein toxins can escape endosomes through the VBC mechanism, and proposed that VBC may be the mechanism that drives the bacterial TAT and eukaryotic UPS systems.
ACCOUNTS OF CHEMICAL RESEARCH
(2022)
