Journal
BIOMICROFLUIDICS
Volume 7, Issue 6, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.4832095
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Funding
- NSF CBET [1065652]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1065652] Funding Source: National Science Foundation
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Plasmonic hot spots, generated by controlled 20-nm Au nanoparticle (NP) assembly, are shown to suppress fluorescent quenching effects of metal NPs, such that hair-pin FRET (Fluorescence resonance energy transfer) probes can achieve label-free ultra-sensitive quantification. The micron-sized assembly is a result of intense induced NP dipoles by focused electric fields through conic nanocapillaries. The efficient NP aggregate antenna and the voltage-tunable NP spacing for optimizing hot spot intensity endow ultra-sensitivity and large dynamic range (fM to pM). The large shear forces during assembly allow high selectivity (2-mismatch discrimination) and rapid detection (15 min) for a DNA mimic of microRNA. (C) 2013 AIP Publishing LLC.
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