Article
Biochemical Research Methods
Yen-Ling Fang, Chih-Hung Wang, Yi-Sin Chen, Chun-Chih Chien, Feng-Chih Kuo, Huey-Ling You, Mel S. Lee, Gwo-Bin Lee
Summary: The study developed an integrated microfluidic chip for rapid isolation of bacteria from blood, aiding in early sepsis diagnosis. The device is efficient with a high capture rate of 85% and the entire detection process can be completed in just 4 hours.
Article
Nanoscience & Nanotechnology
Arash Yahyazadeh Shourabi, Navid Kashaninejad, Mohammad Said Saidi
Summary: This study presents an integrated concentration gradient generator device for providing mechanical stimuli to cells, designed and optimized using numerical simulation, and quantified the device performance using experimental data.
JOURNAL OF SCIENCE-ADVANCED MATERIALS AND DEVICES
(2021)
Article
Chemistry, Multidisciplinary
Xiaorui Wang, Gaowa Xing, Nan Li, Yaoshuang Xie, Ling Lin
Summary: An integrated microfluidic device combining a microfluidic chip with immunochromatographic strips was developed for rapid detection of multiple antibiotics in milk. The device achieved qualitative and quantitative analysis of four types of antibiotics using mobile phone photography and mobile phone application analysis in a 10-minute detection time. The device successfully integrated separation and real-time detection onto a chip, providing a promising perspective for the detection of multiple antibiotics in milk.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Analytical
Yuting Shang, Gaowa Xing, Xuejiao Liu, Haifeng Lin, Jin-Ming Lin
Summary: A portable microfluidic biosensor has been developed for the detection of E. coli O157:H7 using finger actuation. The biosensor consists of three functional zones and is capable of separating and detecting the target bacteria through immunomagnetic separation, nucleic acid extraction and purification, and signal detection. It offers quantitative detection and high recovery rate, eliminating the need for external pumps and skilled personnel.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Wei Liu, Zihan Wang, Zhongping Liu, Junxue Chen, Lingyan Shi, Longjin Huang, Yu Liu, Sheng Cui, Xuan He
Summary: This article presents a method that combines surface-enhanced Raman scattering (SERS) technique with digital microfluidics (SERS-DMF) for automated, high-throughput, and high-sensitivity detection of explosives. The DMF chip, designed with 40 drive electrodes and 8 storage electrodes, was loaded with different concentrations of target molecules, silver nanoparticles, and salts for controlled droplet aggregation, incubation, and detection processes. The SERS-DMF platform exhibited better reproducibility and higher efficiency compared to nonautomatic operations, making it a promising technique for detecting hazardous substances.
Article
Chemistry, Multidisciplinary
Wei Liu, Zihan Wang, Zhongping Liu, Junxue Chen, Lingyan Shi, Longjin Huang, Yu Liu, Sheng Cui, Xuan He
Summary: A method combining surface-enhanced Raman scattering (SERS) with digital microfluidics (DMF) was proposed for automated, high-throughput, and high-sensitivity detection of explosives. By designing a DMF chip and controlling droplet aggregation, incubation, and detection processes using the SERS-DMF platform, the method achieved high detection sensitivity and throughput, with detection limits of 10-7 and 10-8 M for explosives.
Article
Chemistry, Analytical
Yan Man, Meijing Ban, Xinxin Jin, An Li, Jing Tao, Ligang Pan
Summary: A portable and equipment-free microfluidic aptasensor was developed for on-site quantitative detection of Salmonella. This aptasensor utilized microcolumns-based vortex micromixing and incubation, nitrocellulose membranes with single-strand binding proteins (SSBP-NCMs) for enrichment and separation, and distance-readout for visual quantitative detection. The aptasensor showed good specificity and reliability, and could detect Salmonella typhimurium as low as 3.7 x 101 cfu mL-1.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Chemistry, Analytical
Yu-Jia Wei, Ya-Nan Zhao, Xuan Zhang, Xing Wei, Ming-Li Chen, Xu-Wei Chen
Summary: Nowadays, biochemical analysis, such as cell analysis, disease diagnosis and treatment, environmental monitoring, and food safety, is receiving increasing attention. Optically integrated microfluidic technology combines the benefits of high sensitivity, fast detection, and excellent selectivity. By utilizing flexible optical detection techniques and precisely-controlled fluidics, microfluidic chips enable high throughput analysis, reduced reagent consumption, and decreased assay expenditure. This review introduces the integration of microfluidic chips with various optical detection techniques and their applications in biochemical analysis, and also discusses the future prospects of optically integrated microfluidic chips.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Chungen Qian, Yujin Xiao, Jie Wang, Yiwei Li, Shunji Li, Bo Wei, Wei Du, Xiaojun Feng, Peng Chen, Bi-Feng Liu
Summary: Exosomes, along with their associated proteins and nucleic acids, are recognized as biomarkers for tumor diagnosis and prognosis. Detecting exosomes is challenging due to their small size and low concentration. A low-cost, rapid, and portable agarose-based microfluidic chip for exosome concentration and in situ exosomal microRNA detection was developed, providing a new avenue for early cancer detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Kai Zhao, Penglu Zhao, Jianhong Dong, Yunman Wei, Bin Chen, Yanjuan Wang, Xinxiang Pan, Junsheng Wang
Summary: This study demonstrates the successful isolation of circulating tumor cells (CTCs) from blood cells using a combination of dielectrophoresis and magnetophoresis in a microfluidic chip. The results show the potential application of this method in diagnostics.
Article
Spectroscopy
Mubashir Hussain, Jun Zou, Xiaolong Liu, Ronggui Chen, Shuming Tang, Zhili Huang, Jialang Zhuang, Lijun Zhang, Yongjun Tang
Summary: This study proposes a novel pathogen detection system based on cultivating microdroplets and acquiring scattered light signals using a microfluidic device. The microdroplets are initially generated and incubated to cultivate bacteria. Then, the incubated droplets are reinjected into the detection module and passed through laser light to acquire the scattered light signals. The features obtained are trained using machine learning classifiers, and the k-nearest neighbors algorithm demonstrates superior classification performance with 95.6% accuracy for identifying P. aeruginosa.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Review
Chemistry, Analytical
Shuang Ding, Haijun Zhang, Xuemei Wang
Summary: Lung diseases pose serious threats to human health worldwide, and traditional models are unable to accurately simulate lung conditions. Human organ-on-a-chip platforms and biosensors can better mimic lung characteristics, providing new methods for studying disease development and drug efficacy.
Article
Chemistry, Analytical
Myrto Kyriaki Filippidou, Aris Ioannis Kanaris, Evangelos Aslanidis, Annita Rapesi, Dimitra Tsounidi, Sotirios Ntouskas, Evangelos Skotadis, George Tsekenis, Dimitris Tsoukalas, Angeliki Tserepi, Stavros Chatzandroulis
Summary: The presence of heavy metal ions in soil, air and water is a significant global environmental threat, contributing to the rise of chronic diseases. Efforts have been made to detect these ions, but there is a need for sensitive, low-cost, and portable devices for on-site detection. In this study, we combine microfluidic technology and electrochemical sensing in a plastic chip to selectively detect heavy metal ions using DNAzymes immobilized between platinum nanoparticles (PtNPs), providing a reliable portable solution for water pollution monitoring. A fast and easy fabrication method based on the photolithography of dry photosensitive layers is proposed for the realization of the microfluidic-based detection device. Pb2+ ions detection is demonstrated as a proof of concept using the prototype microfluidic device.
Article
Chemistry, Analytical
Haiying Shen, Lianhua Dong, Yunhua Gao, Xia Wang, Xinhua Dai
Summary: In this study, a highly integrated microfluidic chip for nucleic acid extraction, amplification, and detection was designed. A hand-held device with precise temperature control and high-quality imaging was developed for experiment and data analysis. The chip allows for mobile point-of-care testing via wireless communication with a smartphone. The results show the potential of this platform for nucleic acid detection.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yan Jin, Jingyi Wang, Zhiqiang Wang, Peng Xiong, Jianing Cheng, Tongyu Xu
Summary: This paper presents an integrated microfluidic biosensing system for rapid and sensitive detection of Salmonella typhimurium. The system consists of a microfluidic chip with a versatile valve, recombinase polymerase amplification (RPA) for nucleic acid detection, and a customized real-time fluorescence detection system. The system exhibits the potential to detect Salmonella at concentrations as low as 1.0 x 10(2) copies/mu L within 30 min, consistent with real-time quantitative polymerase chain reaction (qPCR) tests. The versatile valve in this integrated microfluidic biosensing system holds significant potential for on-site detection of foodborne pathogens.
Article
Engineering, Environmental
Ruey-Jen Yang, Chin-Chung Tseng, Wei-Jhong Ju, Hsiang-Li Wang, Lung-Ming Fu
CHEMICAL ENGINEERING JOURNAL
(2018)
Article
Nanoscience & Nanotechnology
Van-Phung Mai, Cheng-Hao Ku, Ruey-Jen Yang
MICROFLUIDICS AND NANOFLUIDICS
(2019)
Article
Energy & Fuels
Yu-Yuan Sun, Van-Phung Mai, Ruey-Jen Yang
Article
Nanoscience & Nanotechnology
Woon-Shing Yeung, Fu-Yao Yang, Ruey-Jen Yang
Article
Physics, Applied
Woon-Shing Yeung, Van-Phung Mai, Ruey-Jen Yang
PHYSICAL REVIEW APPLIED
(2020)
Article
Physics, Applied
Fu-Yao Yang, Fu-Sui Hung, Woon-Shing Yeung, Ruey-Jen Yang
Summary: This study proposes an optimization method for the design of planar arbitrary-geometry thermal cloaks using natural materials based on the bilayer theory in thermal-cloaking studies. The method aims to provide a practical design method for improved thermal-cloaking performance.
PHYSICAL REVIEW APPLIED
(2021)
Article
Chemistry, Analytical
Wei-Hao Huang, Van-Phung Mai, Ruo-Yin Wu, Ko-Li Yeh, Ruey-Jen Yang
Summary: This study proposes a sensor for detecting mercury and lead ions using graphene oxide and aptamer solution, with detection limits lower than the maximum limits set by the World Health Organization for drinking water.
Article
Chemistry, Multidisciplinary
Van-Phung Mai, Wei-Hao Huang, Ruey-Jen Yang
Summary: Thermo-osmotic energy conversion using waste heat is a method of harvesting sustainable energy while reducing environmental impacts. This study investigates the effect of surface charge and pH value on thermo-osmotic performance through numerical simulation, and finds that pH level has a significant impact on enhancing the thermo-osmotic conversion efficiency.
Article
Thermodynamics
Van-Phung Mai, Tsung-Yu Lee, Ruey-Jen Yang
Summary: This study fabricated different types of droplet-triboelectric nanogenerators and investigated the effect of electrowetting-assisted charge injection on their performance. The results showed that composite materials doped with silk powder and polytetrafluoroethylene particles significantly increased the output current, and further performance enhancement could be achieved through appropriate design of the dielectric layer and operating conditions.
Article
Engineering, Chemical
Van-Phung Mai, Wei-Hao Huang, Yong-Lin Chang, Ruey-Jen Yang
Summary: This study investigates the capillary-driven energy conversion performance of a 2D composite membrane consisting of a graphene oxide (GO) matrix and natural silk fibers. The results show that the composite structure of the GO@silk membrane provides enhanced physical robustness and improved ion selectivity. The experimental results demonstrate high voltage and current outputs of 0.22 V and 12 μA, respectively, indicating the feasibility of using composite membrane structures for environmental energy conversion on a larger scale.
JOURNAL OF MEMBRANE SCIENCE
(2023)
Article
Thermodynamics
Wei-Hao Huang, Chih-Chang Chang, Ruey-Jen Yang
Summary: This study conducted numerical simulations to investigate the thermoelectric response in nanofluidic channels with slip mechanism. The results showed that temperature-dependent ion electrophoretic mobility and thermoosmosis dominate the thermoelectric performance. This study provides valuable insights for developing efficient nanofluidic-based thermoelectric generators.
INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER
(2023)
Article
Mechanics
Woon-Shing Yeung, Fu-Sui Hung, Van-Phung Mai, Ruey-Jen Yang
Summary: This paper presents a theoretical analysis of steady-state creeping flow in a composite porous domain with coupling heat transfer. The findings have important applications in thermal cloaking studies.
JOURNAL OF MECHANICS
(2022)
Review
Engineering, Environmental
Van-Phung Mai, Wei-Hao Huang, Ruey-Jen Yang
Summary: Blue energy, also known as salinity gradient energy, is considered a highly attractive solution for sustainable clean energy generation through ion transport via nanopassages like nanopores. This review discusses opportunities and challenges in ion transport through membranes of different dimensions, introduces strategies for enhancing ion transport and system performance, and explores mechanisms for improving ion transport through nanopores in the pursuit of harnessing blue energy efficiently in the future.
ACS ES&T ENGINEERING
(2021)
Article
Mechanics
Mahalakshmi Thangavelu, Nithyadevi Nagarajan, Hakan F. Oztop, Ruey-Jen Yang
Summary: Simulations were conducted to investigate magnetohydrodynamic convection of an Ag-water nanofluid in an inclined enclosure with a center heater, showing that heat transfer performance is influenced by the inclination angle and Hartmann number. Optimal heat transfer performance was achieved under specific conditions, and the heat transfer performance improves with an increasing volume fraction of nanoparticles.
JOURNAL OF MECHANICS
(2021)
Article
Chemistry, Multidisciplinary
Van-Phung Mai, Ruey-Jen Yang