Article
Chemistry, Physical
Haifeng Sha, Bing Yan
Summary: A fluorescence resonance energy transfer (FRET) aptasensor was developed in this study for quantitatively detecting 17 beta-Estradiol (E2). By utilizing metal organic frameworks and specific bonding between avidin and biotin, the sensor showed sensitive detection of E2 and has potential for rapid detection of other small biological molecules.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Analytical
Zhenzhen Jia, Kangsheng Tu, Qiuran Xu, Wenhui Gao, Cui Liu, Biyun Fang, Mingzhen Zhang
Summary: A universal sensing platform based on DNA-Ag NC was developed for sensitive detection of disease-associated nucleic acids, showing excellent reproducibility, selectivity, and potential applications in human serum samples. The platform was successfully applied for miR-362 and HIV-related DNA detection, achieving low detection limits of 6.5 nM and 1.7 nM, respectively.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Qiaoling Ding, Wanwei Qiu, Chunxue Sun, Hongxin Ren, Guodong Liu
Summary: Lateral flow nucleic acid biosensors (LFNABs) have garnered widespread attention for their quick response time, affordability, and visible results. The preparation of DNA-gold nanoparticle (DNA-AuNP) conjugates is a crucial step in developing LFNABs and significantly impacts their sensitivity. Various conjugation methods, such as the salt-aging method, microwave-assisted dry heating method, freeze-thaw method, low-pH method, and butanol dehydration method, have been reported. In this study, we compared the analytical performances of LFNABs prepared using these five methods and found that the butanol dehydration method exhibited the lowest detection limit. The optimized LFNAB prepared with this method achieved a detection limit of 5 pM for single-strand DNA, which is 100 times lower than that of the salt-aging method. The resulting LFNAB was successfully applied to detect miRNA-21 in human serum, yielding satisfactory results. Therefore, the butanol dehydration method offers a rapid approach for preparing DNA-AuNP conjugates for LFNABs and can be extended to other types of DNA biosensors and biomedical applications.
Article
Chemistry, Analytical
Qiaoling Liu, Wenjia Bai, Zhihui Guo, Xingwang Zheng
Summary: A novel scheme was developed to enhance the chemical affinity and electric conductivity between Ru(bpy)(3)(2+)-doped silica nanoparticles and carbon nanotubes (CNT) by electrochemically depositing a nanoscale chitosan hydrogel layer on the CNT surface. This scheme improved the ECL effectivity of Ru(bpy)(3)(2+) inside silica nanoparticles, leading to the development of a highly sensitive ECL method for miRNA detection.
Article
Chemistry, Analytical
Wenxin Wu, Jie Wu, Hao Huang, Bin Qiao, Chenglong Jiang, Yao Shi, Chao Wang, Hua Pei, Qiqi Xu, Xiaotian Wu, Qiang Wu, Huangxian Ju
Summary: In this work, an electrochemiluminescence (ECL) biosensing strategy was proposed for sensitive detection of 5-methyl-cytosine (5mC) DNA methylation. The biosensor utilized encapsulated [Ru(byp)3]2+ in silica nanoparticles (Ru@SiO2) as labeling agents for signal amplification. By immobilizing biotinylated DNA on streptavidin-modified magnetic microbeads (S-MBs), sequentially methylated ssDNA, 5mC antibody, and Ru@SiO2 labeled antibody were captured and separated from other species. The biosensor demonstrated high sensitivity, convenience, automation, versatility, and potential for detecting important gene-specific methylation without nucleic acid amplification and sample pretreatment.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Physical
Agustina Cotic, Koen Veys, Daniel Escudero, Alejandro Cadranel
Summary: This study demonstrates the presence of high kinetic barriers for internal conversion in anti-Kasha chromophores and provides guidelines for their design. The results highlight the importance of wavefunction symmetry in inhibiting dissipative IC and offer insights into the dissipation process at the atomistic level.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Materials Science, Ceramics
Lin Liu, Hao Sheng, Jian Xu, Gang Fang, Qingbo Xu
Summary: Ru(bpy)(3)(2+)-doped TiO2-SiOR hybrid films were prepared by the sol-gel method using different precursors, forming Ti-O-Si and Si-O-Si bonds through sol-gel processing. UV-curing broke C-O-C cycles and C=C double bonds for photo-polymerization. The absorption edge of the hybrid films shifted to around 300nm, with high transmittance up to 2500nm. Photoluminescent spectra showed differences in intensity and emission peaks between hosts TiO2-SiOR1 and TiO2-SiOR2, with thermal decomposing temperatures at around 408°C and 411°C, respectively.
JOURNAL OF NON-CRYSTALLINE SOLIDS
(2021)
Article
Chemistry, Analytical
Lihong Gao, Yitian Huang, Huizhu Ren, Sisi Chen, Shupei Zhang, Hong Dai
Summary: A synergistic amplified electrochemiluminescent (ECL) immunoassay was developed for ultra-sensitive detection of thyroglobulin by utilizing a self-enhanced signal tag and sensing substrate with satisfactory photothermal property. With the self-enhanced and photo-thermal synergistic amplified strategy, the proposed ECL sensor achieved a wide linear range and low detection limit.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Sanjay Yadav, Nishu Choudhary, Vasavdutta Sonpal, Alok Ranjan Paital
Summary: The simultaneous detection and remediation of highly toxic mercury-ion are urgently needed for environmental benefits. A new composite material, CD@DFNS@SH, is developed for selective turn-on red luminescence with a limit of detection of 5 nM. This material also exhibits high adsorption capacity for mercury remediation (695 mg g-1) and can be used for fluorescence imaging of mercury-ion in living organisms.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Kai Sun, Xueqi Wang, Yuxin Qu, Han Wang, Jing Cheng
Summary: The development of more accurate and adaptive molecular diagnosis technologies has been fostered by the emergence of the global pandemic and the discovery of nucleic acid biomarkers in cancer diagnosis. Current nucleic acid testing methods either lack sensitivity or require tedious amplification operations, which cannot meet the demand for point-of-care diagnosis. A fluorescence one-step-bDNA-based lateral flow assay (FOB-LFA) method for amplification-free nucleic acid testing is presented here, which demonstrated high sensitivity and specificity for SARS-CoV-2 detection, as well as potential for clinical examination. FOB-LFA shows outstanding performance in terms of sensitivity, specificity, cost, portability, and risk reduction, meeting the needs of point-of-care testing for various infectious and genetic diseases.
ANALYTICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Jin-Ha Choi, Taehyeong Ha, Minkyu Shin, Sang-Nam Lee, Jeong-Woo Choi
Summary: Nucleic acids, including DNA and RNA, are promising biomarkers for cancer diagnosis. Nanomaterial-based FRET and MEF biosensors offer accurate and sensitive detection of cancer-related nucleic acids, contributing to early cancer diagnosis.
Article
Chemistry, Analytical
Tao Dong, Ran Yin, Qingcai Yu, Wanwei Qiu, Kun Li, Lisheng Qian, He Li, Bing Shen, Guodong Liu
Summary: The study presents a highly sensitive approach for detecting microRNA-21 (miR-21) in cancer cells and human serum using Au@Si nanocomposite labeled lateral flow assay. After systematic optimization, the method can detect a minimum concentration of 1.0 pM miR-21, which is 60 times lower than that of the GNP-based lateral flow assay.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Jiangwei Li, Yue Hu, Jiao Yang, Yingchun Li
Summary: In this study, [Ru(bpy)(3)](2+) doped mesoporous carbon nanospheres (RMCNs) were synthesized via a one-step approach, and showed enhanced electrochemiluminescence (ECL) emission and stability. The synergistic effects of [Ru(bpy)(3)](2+) doping level, Zeta potential, pore size, and particle size of RMCNs on ECL intensity were systematically studied. By combining with molecularly imprinted polymer (MIP), an ECL sensor with good selectivity and wide detection range for beta-hydroxybutyric acid was developed.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Materials Science, Multidisciplinary
Lin Liu, Hao Sheng, Jian Xu, Gang Fang
Summary: Eu3+ or Ru(bpy)(3)(2+)-doped TiO2-SiOR hybrid films were prepared by the anhydrous sol-gel process for luminescent solar concentrator applications. Different photoluminescent spectra were obtained under different excitation wavelengths for Eu3+-doped films, while the same luminescent peaks occurred at around 588 nm for Ru(bpy)(3)(2+)-doped films, indicating potential photocatalytic applications.
Article
Chemistry, Analytical
Maria Luisa Moya, Francisco Jose Ostos, Izamar Moreno, Diandra Garcia, Paula Moreno-Gordillo, Ivan V. Rosado, Pilar Lopez-Cornejo, Jose Antonio Lebron, Manuel Lopez-Lopez
Summary: Researchers have investigated the potential applications of cationic metallo-liposomes in gene transfection and drug delivery by preparing and characterizing nanostructures. The results demonstrate the successful internalization of these nanostructures into cells, as well as the successful encapsulation of doxorubicin into metallo-liposomes.
Article
Chemistry, Multidisciplinary
Juhong Chen, Samuel D. Alcaine, Angelyca A. Jackson, Vincent M. Rotello, Sam R. Nugen
Article
Chemistry, Analytical
T. C. Hinkley, S. Garing, S. Singh, A-L M. Le Ny, K. P. Nichols, J. E. Peters, J. N. Talbert, S. R. Nugen
Article
Multidisciplinary Sciences
Troy C. Hinkley, Sangita Singh, Spencer Garing, Anne-Laure M. Le Ny, Kevin P. Nichols, Joseph E. Peters, Joey N. Talbert, Sam R. Nugen
SCIENTIFIC REPORTS
(2018)
Article
Chemistry, Analytical
Troy C. Hinkley, Spencer Garing, Paras Jain, John Williford, Anne-Laure M. Le Ny, Kevin P. Nichols, Joseph E. Peters, Joey N. Talbert, Sam R. Nugen
Review
Biochemical Research Methods
Caitlin M. Carmody, Julie M. Goddard, Sam R. Nugen
Summary: Bacteriophages are versatile viruses with potential applications in agriculture and public health. Their capsids can be modified genetically or chemically to enhance specificity and stability for various functions.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Microbiology
Anqi Chen, Danhui Wang, Sam R. Nugen, Juhong Chen
Summary: Utilizing an enzyme-based fluorescent assay, the study demonstrated a sensitive method for detecting the presence of E. coli in food samples, providing a straightforward approach to detect potential biological contamination. The engineered phages showed a detection limit of 10 CFU/mL and could be applied to detect E. coli strains from environmental samples.
Article
Nanoscience & Nanotechnology
Kathryn A. Hufziger, Emma L. Farquharson, Brenda G. Werner, Qingmin Chen, Julie M. Goddard, Sam R. Nugen
Summary: The current techniques for bacteriophage immobilization onto magnetic particles are limited in cost-effectiveness and specificity. This study presents a plasmid-based technique that incorporates a silica-binding protein into the phage capsid, enabling targeted binding to silica without modifying the phage genome. By improving immobilization density, this technique offers a step toward reducing cost and increasing specificity in phage immobilization methods.
ACS APPLIED BIO MATERIALS
(2022)
Article
Microbiology
Emma L. Farquharson, Sam R. Nugen
Summary: The complete genome sequence and annotation of bacteriophage Ac3 is presented in this study. The host range of Ac3 was evaluated using spot tests and plaque assays, revealing its ability to adsorb to 59.7% of Escherichia coli strains and replicate within 37.5% of the strains. This research provides a solid starting point for future investigations.
PHAGE-THERAPY APPLICATIONS AND RESEARCH
(2022)
Article
Microbiology
Caitlin M. Carmody, Emma L. Farquharson, Sam R. Nugen
Summary: This study presents the complete genome sequence and host range determination of phage SV76, highlighting its requirement for specific receptor binding proteins. SV76 is closely related to T2 and exhibits similar host ranges within ECOR, laying a foundation for future studies on T4-like phages and their biotechnological applications.
PHAGE-THERAPY APPLICATIONS AND RESEARCH
(2022)
Article
Education, Scientific Disciplines
Alexandra J. Macbeth, Hannah S. Zurier, Erin Atkins, Sam R. Nugen, Julie M. Goddard
Summary: Connecting the public to STEM concepts is crucial for technological advancement and inspiring future scientists, impacting both the communicator and the audience's understanding of scientific topics. Increasing public awareness about scientific issues through STEM engagement can lead to informed policy and consumer choices, particularly in fields such as food science where new technologies face initial resistance.
JOURNAL OF FOOD SCIENCE EDUCATION
(2021)
Article
Nanoscience & Nanotechnology
Hannah S. Zurier, Michelle M. Duong, Julie M. Goddard, Sam R. Nugen
ACS APPLIED BIO MATERIALS
(2020)
Article
Chemistry, Analytical
Michelle M. Duong, Caitlin M. Carmody, Sam R. Nugen
Article
Cell Biology
Elsi M. Pulkkinen, Troy C. Hinkley, Sam R. Nugen
INTEGRATIVE BIOLOGY
(2019)
Article
Chemistry, Analytical
Danhui Wang, Troy Hinkley, Juhong Chen, Joey N. Talbert, Sam R. Nugen
Article
Biotechnology & Applied Microbiology
Sangita Singh, Troy Hinkley, Sam R. Nugen, Joey N. Talbert
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2018)