Review
Engineering, Chemical
Karin Schroen, Izabella Bouhid de Aguiar
Summary: Membrane filtration plays a crucial role in food ingredient production, but there is a lack of consideration for the specific properties of food ingredients. This review focuses on the processes of concentration and fractionation, highlighting the importance of deformability in membrane filtration.
SEPARATION AND PURIFICATION TECHNOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Miguel Angel Robles-Ramos, Silvia Zorrilla, Carlos Alfonso, William Margolin, German Rivas, Begona Monterroso
Summary: Biomolecular condensation through phase separation is a novel mechanism for regulating bacterial processes, including cell division. It has been found that FtsZ alone can also form condensates under certain conditions, depending on its binding state and the crowded environment of the cytoplasm. This mechanism may have a more widespread role in bacterial division than previously thought.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Engineering, Chemical
Mayank Kumar, Narasimha Mangadoddy, R. Banerjee, Gaekwad E. Sreedhar, Shiv Kumar Raparla, Rajan Kumar
Summary: An open source high fidelity LES based algebraic slip mixture multiphase flow solver was developed to tackle the high compute cost of multiphase simulations for dense medium cyclones, achieving a significant speed up on a general purpose GPU compared to a CPU implementation. The performance of the solver was validated with experimental results, and the implications of key operating variables on DMC operation were studied, providing insights into the medium segregation based on force analysis. The response of the DMC in terms of hydrodynamic force analysis and density differential with varying design and operating variables was discussed, suggesting that a lower pressure head is preferred for DMCs used in mineral processing.
MINERALS ENGINEERING
(2021)
Review
Engineering, Chemical
Ioannis H. Karampelas, Jenifer Gomez-Pastora
Summary: The demand for precise separation of particles, cells, and other biological matter has increased in recent years, leading to increased scientific interest in the topic. This article establishes the theoretical basis for fluid flow and particle separation and summarizes computational work done in the field of particle and cell separation, with a focus on magnetic, dielectric, and acoustic methods.
Review
Materials Science, Multidisciplinary
Sanghyun Lee, Hojin Kim, Sung Yang
Summary: The separation of blood components is essential for biology, clinical diagnosis, therapeutics, and personalized medicine. Microfluidics has become a popular technology for this purpose, allowing for the separation of target cells based on their intrinsic properties. This review discusses various microfluidic-based hydrodynamic cell-separation technologies, categorizing them according to their working principles. The relative performances and features of these technologies are thoroughly discussed, along with future perspectives on commercialization and standardization for widespread use and applications.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Biochemical Research Methods
Haizhen Sun, Yukun Ren, Ye Tao, Tianyi Jiang, Hongyuan Jiang
Summary: The study introduces a technique called alternating current electrothermal-flow field-effect transistor (ACET-FFET) for in-droplet cell/particle concentration. By tuning the electric field and splitting droplets, cells/particles can be flexibly and efficiently concentrated. This technology demonstrates broad applications in droplet microfluidics.
Article
Engineering, Chemical
Xiangzhi Zhang, Xiawei Xu, Yong Ren, Yuying Yan, Aiguo Wu
Summary: An Y-Y shaped microfluidic device was designed to separate NSCLC cells using a dielectrophoresis approach, with a separation efficiency of around 99% achieved under optimal operating conditions. This study presents a potentially efficient, facile, and low-cost route for circulating tumor cell separation, which was optimized through numerical simulations.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Tomoki Murakami, Keiichiro Koiwai, Hiroaki Suzuki
Summary: The study demonstrated the effectiveness of using the DLD method to separate hemocytes of shrimp based on size, allowing for the analysis of immune-related gene expression differences between cell populations. This microfluidics-based technology proves to be a valuable tool for studying nonmodel organisms with poorly established cell characteristics.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Hossein Tavassoli, Prunella Rorimpandey, Young Chan Kang, Michael Carnell, Chris Brownlee, John E. Pimanda, Peggy P. Y. Chan, Vashe Chandrakanthan
Summary: A label-free method using inertial microfluidics is developed to purify viable cardiomyocytes from mouse neonatal hearts with purities over 90%. The isolated cardiomyocytes retain their identity and function, exhibiting different physico-mechanical properties compared to non-cardiomyocytes. This method could be a valuable tool for advancing the understanding of cardiomyocyte identity and function, with potential benefits for cell therapy and diagnostic applications.
Article
Chemistry, Analytical
Georgii Grigorev, Nikolay O. Nikitin, Alexander Hvatov, Anna Kalyuzhnaya, Alexander Lebedev, Xiaohao Wang, Xiang Qian, Georgii Maksimov, Liwei Lin
Summary: This paper presents a generative design methodology for a micro hydrodynamic single-RBC trap, aiming to improve the through-slit flowrates for effective cell trapping in microfluidics-based single-cell analysis. The design, validated with experimental data, utilizes an evolutionary algorithm to generate optimized L-shaped trapping slits, leading to increased flow velocities and improved trapping efficiency for cells of various sizes.
Article
Chemistry, Analytical
Phi Hung Hoang, Islam Seder, Ana Isabel Ferrer Ramirez, Sung-Jin Kim
Summary: This study presents a fully automated and effective fluidic system for the isolation and detection of cfDNA from whole blood. The system utilizes disposable chambers and key components such as the sedimentation chamber and plunger chamber to achieve plasma separation and cfDNA capture, followed by detection using real-time PCR on a chip.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Nanoscience & Nanotechnology
Xiaofei Yuan, Andrew Glidle, Hitoshi Furusho, Huabing Yin
Summary: Traditional two-dimensional fluid control strategies in microfluidic cell sorting have limitations in terms of precision and purity, while devices based on three-dimensional hydrodynamics offer better flow-focusing characteristics but are restricted by arbitrary sample positioning. The 3D hydrodynamic focusing sorting platform designed in this study has advantages in precisely controlling sample velocity and position, showing potential for high-accuracy Raman activated sorting.
MICROFLUIDICS AND NANOFLUIDICS
(2021)
Article
Biochemical Research Methods
Federica Torrisi, Giovanna Stella, Francesca M. Guarino, Maide Bucolo
Summary: This paper presents a combination of a cell counting algorithm and a velocity algorithm based on a Digital Particle Image Velocimetry (DPIV) method to study the collective behavior of micro-particles in response to hydrodynamic stimuli. A wide experimental campaign was conducted using micro-particles of different natures and diameters, such as living cells and silica beads. The proposed data analysis procedure based on the DPIV-based algorithm and a custom counting algorithm allows for the extrapolation of micro-particles velocities and the obtainment of the instantaneous micro-particles number.
Article
Biochemistry & Molecular Biology
David Tichy, Zdenek Slouka
Summary: The article introduces an electrodialysis method for ion separation using an electric field-mediated process. Through a microfluidic system and experiments with a model KCl solution, the system's working principle and performance are demonstrated, showing the flexibility to select different operating modes based on the needs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Mechanics
Md Ehtashamul Haque, Amirali Matin, Xu Wang, Maiwenn Kersaudy-Kerhoas
Summary: The study reveals that syringe pump fluctuations can have a negative impact on microfluidic separation, especially in devices based on the cell-free layer concept. It was found that pressure fluctuations tend to stabilize at higher flow rates. This work provides insights for the design and improvement of microfluidic systems in resisting pulsating or fluctuating flow conditions.
Article
Biotechnology & Applied Microbiology
Yuya Yajima, Chu Ning Lee, Masumi Yamada, Rie Utoh, Minoru Seki
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2018)
Article
Engineering, Chemical
Masumi Yamada, Minoru Seki
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
(2018)
Article
Chemistry, Analytical
Hideki Iwadate, Masumi Yamada, Naoki Kimura, Rina Hashimoto, Yuya Yajima, Rie Utoh, Minoru Seki
SENSORS AND ACTUATORS B-CHEMICAL
(2019)
Article
Chemistry, Analytical
Takuma Yanai, Takatomo Ouchi, Masumi Yamada, Minoru Seki
Article
Chemistry, Analytical
Shuhei Aoyama, Kenji Monden, Yuto Akiyama, Masumi Yamada, Minoru Seki
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Analytical
Tinglin Mu, Hajime Toyoda, Yuki Kimura, Masumi Yamada, Rie Utoh, Daisuke Umeno, Minoru Seki
ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Kotone Saeki, Hisataka Hiramatsu, Ayaka Hori, Yu Hirai, Masumi Yamada, Rie Utoh, Minoru Seki
Article
Biotechnology & Applied Microbiology
Akihiro Morita, Masumi Yamada, Rie Utoh, Kanta Momiyama, Hideki Iwadate, Minoru Seki
Summary: This study reports a new approach using fibrillized collagen microparticles (F-CMPs) as intercellular binders to form hepatocyte-based 3D tissues. Thick and stable tissues were successfully created by mixing these microparticles with hepatocytes, with a thickness of approximately 200 microns. The incorporation of F-CMPs improved cell viability and hepatocyte-specific functions, demonstrating the significance of this method for hepatocyte-based tissue engineering.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Materials Science, Biomaterials
Rie Utoh, Sakiko Enomoto, Masumi Yamada, Keigo Yamanaka, Yuya Yajima, Kazuya Furusawa, Minoru Seki
Summary: A unique process was proposed in this study to fabricate fragmented collagen microfibers using a microfluidic laminar-flow system, which can reconstitute the 3D microenvironments surrounding hepatocytes by controlling the morphology. The fragmented fibers enhanced the survival and functions of hepatocytes, promoting the formation of cell-cell and cell-matrix interactions.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Biochemical Research Methods
Mai Takagi, Masumi Yamada, Rie Utoh, Minoru Seki
Summary: In this study, a multiscale microfluidic system was developed for the perfusion culture of spheroids, addressing the limitation of diffusion-based oxygen/nutrient transport. The system allows for size-controlled formation of spheroids and direct medium supply, making it suitable for precision cell culture in drug development, disease modeling, and regenerative medicine.
Article
Chemistry, Analytical
Natsumi Shimmyo, Makoto Furuhata, Masumi Yamada, Rie Utoh, Minoru Seki
Summary: This study presents a simple, scalable, and efficient method to physically capture circulating tumor cells (CTCs) using a microfluidic isolator with microslit channels. The wide but thin microslit channels selectively capture CTCs while allowing other blood cells to flow through, resulting in a highly effective and easy-to-use system. The effects of microchannel geometry and operating parameters on capture efficiency and selectivity were investigated, and the system's applicability to clinical cancer diagnosis was demonstrated.
Article
Biochemical Research Methods
Ryoken Ozawa, Hideki Iwadate, Hajime Toyoda, Masumi Yamada, Minoru Seki
Article
Nanoscience & Nanotechnology
Aruto Hori, Yuki Watabe, Masumi Yamada, Yuya Yajima, Rie Utoh, Minoru Seki
ACS APPLIED BIO MATERIALS
(2019)
Article
Chemistry, Multidisciplinary
Mayu Fukushi, Keita Kinoshita, Masumi Yamada, Yuya Yajima, Rie Utoh, Minoru Seki
Article
Chemistry, Analytical
Shuhei Aoyama, Yuto Akiyama, Kenji Monden, Masumi Yamada, Minoru Seki